| 研究課題/領域番号 |
22KJ3112
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| 補助金の研究課題番号 |
21J40229 (2021-2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2021-2022) |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分90120:生体材料学関連
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| 研究機関 | 国立研究開発法人物質・材料研究機構 |
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研究代表者 |
Abdelaleem Shimaa 国立研究開発法人物質・材料研究機構, 高分子・バイオ材料研究センター, 特別研究員(RPD)
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| 研究期間 (年度) |
2023-03-08 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円)
2023年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2022年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2021年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
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| キーワード | microtubules / actin / Leader cells / collective migration / Mechanobiology / Viscoelastic material / EMT / Actomyosin / ECM concentration / nanoparticle / AFM |
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| 研究開始時の研究の概要 |
Viscoelastic substrates and cRGD concentrations are used to identify the cytoskeletal systems coordination and crosstalk. These surfaces with diversified biomechanical properties are used to determine the crosstalk and functionalities of actin in the absence and presence of MTs. The visualization of targeted proteins, RNA silencing, transfection and rt-PCR would be utilized to achieve this purpose. Moreover, the establishment of stable cell lines with specific fret sensors would be pursued.
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| 研究実績の概要 |
Studying biological processes with the help of biomaterials that mimic both mechanical and biochemical cues is an effective way to understand cellular responses, especially those related to the cytoskeletal system. To achieve this goal, we have used cRGD concentration as a biochemical cue to investigate the relationship between MTs and cellular mechanics and morphology. Our study has shown that the contribution of MTs to cellular stiffness and morphology is dependent on the underlying cRGD densities. (These results have been submitted, and the research article is currently being revised.)
In collaborative research, we employed photoactivatable surfaces to explore the influence of confinement, among other environmental stimuli like cellular phenotypes and cytoskeletal system perturbations, on the stress inside living cells using atomic force microscopy. Our results indicate that changes in intracellular prestress occurs in accordance with the environmental stimuli. This study was published STAM 2023 and selected as news research highlight in several websites.
Additionally, the photoactivatable surfaces have been utilized to show the effect of alteration of ECM compositions on the collective cell migration and leader cell formation, we showed a new correlation between leader cells in collective migration and the biochemical signals that arise from underlying extracellular matrix proteins (These results have been submitted, and the research article is currently being revised.; preliminary results were presented at two conferences)
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