蛋白相互作用阻害によるWNKキナーゼ阻害薬の効率的スクリーニング

• 研究課題/領域番号: 23659439
• 研究種目: 挑戦的萌芽研究
• 配分区分: 基金
• 研究分野: 腎臓内科学
• 研究機関: 東京医科歯科大学
• 研究代表者: 内田 信一 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (50262184)
• 研究期間 (年度): 2011 – 2012
• 研究課題ステータス: 完了 (2012年度)
• 配分額: 3,640千円 (直接経費: 2,800千円、間接経費: 840千円); 2012年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円); 2011年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
• キーワード: 水 / 電解質代謝学 / リン酸化酵素 / ケミカルライブラリー / 高血圧 / キナーゼ

研究概要

血圧調節におけるWNKシグナルカスケードの重要性に着目し、新規高血圧治療薬開発を目的としてシグナル分子間相互作用阻害薬探索を試みた。蛍光相関分光法を用いたハイスループット・スクリーニング系を確立し、17,000種類のライブラリーから、2種類の有望リード化合物を得た。医薬品としての最適化を図るため化合物修飾による誘導体展開を実施し、リード化合物よりも優れ、生体内でも阻害活性を示す新規化合物の創出に成功した。

研究成果

• [雑誌論文] Characterization of AQP-2 gene mutation (R254Q) in a family with dominant nephrogenic DI.. (2013)
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  • DOI: 10.1111/j.1442-200x.2012.03614.x
  • 査読あり
• [雑誌論文] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension. (2013)
  • 著者名/発表者名: Wakabayashi M
  • 雑誌名: Cell Rep. 巻: 3 ページ: 858-868
  • 査読あり
• [雑誌論文] Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells (2012)
  • 著者名/発表者名: Hara-Chikuma M, Sugiyama Y, Kabashima K, Sohara E, Uchida S, Sasaki S, Inoue S, Miyachi Y
  • 雑誌名: FASEB J 巻: 26 ページ: 211-8
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates itsubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 425 ページ: 456-61
  • 査読あり
• [雑誌論文] A new role for aquaporin 7 in insulinsecretion (2012)
  • 著者名/発表者名: Louchami K, Best L, Brown P, Virreira M, Hupkens E, Perret J, Devuyst O, Uchida S, Delporte C, Malaisse WJ, Beauwens R, Sener A
  • 雑誌名: Cell Physiol. Biochem 巻: 29 ページ: 65-74
  • 査読あり
• [雑誌論文] ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney (2012)
  • 著者名/発表者名: Naguro I, Umeda T, Kobayashi Y, Maruyama J, Hattori K, Shimizu Y, Kataoka K, Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
  • 雑誌名: Nat. Commun 巻: 3 ページ: 1285-1285
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice (2012)
  • 著者名/発表者名: Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S
  • 雑誌名: Hypertension 巻: 60 ページ: 981-90
  • 査読あり
• [雑誌論文] Accumulation of autofluorescent storage material in brain is accelerated by ischemia in chloride channel 3 gene-deficient mice (2012)
  • 著者名/発表者名: Ohtaki H, Ohara K, Song D, Miyamoto K, Tsumuraya T, Yofu S, Dohi K, Tanabe S,Sasaki S, Uchida S, Matsunaga M, Shioda S
  • 雑誌名: J. Neurosci. Res 巻: 90 ページ: 2163-72
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Misawa M, ChigaM, Alessi DR, Sasaki S, Uchida S
  • 雑誌名: Biol.Open 巻: 1 ページ: 120-7
  • 査読あり
• [雑誌論文] Uchida S.Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels (2012)
  • 著者名/発表者名: Susa K, Kita S, Iwamoto T, Yang SS, LinSH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR
  • 雑誌名: Clin. Exp. Nephrol 巻: 16 ページ: 530-8
  • 査読あり
• [雑誌論文] Uchida S.WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone.Biochem (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, RaiT, Sasaki S
  • 雑誌名: Biophys. Res. Commun 巻: 427 ページ: 743-7
  • 査読あり
• [雑誌論文] Does a β2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S, Chiga M, Sohara E, Rai T, Sasaki S
  • 雑誌名: Nat. Med 巻: 18 ページ: 1324-5
  • 査読あり
• [雑誌論文] Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells. (2012)
  • 著者名/発表者名: Hara-Chikuma M
  • 雑誌名: FASEB J. 巻: 26 号: 1 ページ: 211-218
  • DOI: 10.1096/fj.11-186627
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 425 号: 2 ページ: 456-461
  • DOI: 10.1016/j.bbrc.2012.07.124
  • 査読あり
• [雑誌論文] A new role for aquaporin 7 in insulin secretion. (2012)
  • 著者名/発表者名: Louchami K
  • 雑誌名: Cell Physiol. Biochem. 巻: 29 号: 1-2 ページ: 65-74
  • DOI: 10.1159/000337588
  • 査読あり
• [雑誌論文] ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney. (2012)
  • 著者名/発表者名: Naguro I., et al.
  • 雑誌名: Nature Communications 巻: 3 号: 1 ページ: 1285-1285
  • DOI: 10.1038/ncomms2283
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice. (2012)
  • 著者名/発表者名: Nishida H
  • 雑誌名: Hypertension 巻: 60 号: 4 ページ: 981-990
  • DOI: 10.1161/hypertensionaha.112.201509
  • 査読あり
• [雑誌論文] Accumulation of autofluorescent storage material in brain is accelerated by ischemia in chloride channel 3 gene-deficient mice. (2012)
  • 著者名/発表者名: Ohtaki H
  • 雑誌名: J. Neurosci. Res. 巻: 90 号: 11 ページ: 2163-2172
  • DOI: 10.1002/jnr.23110
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K
  • 雑誌名: Biology Open 巻: Vol.1 号: 2 ページ: 120-127
  • DOI: 10.1242/bio.2011048
  • 査読あり
• [雑誌論文] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels. (2012)
  • 著者名/発表者名: Susa K
  • 雑誌名: Clin. Exp. Nephrol. 巻: 16 ページ: 530-538
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 427 号: 4 ページ: 743-747
  • DOI: 10.1016/j.bbrc.2012.09.130
  • 査読あり
• [雑誌論文] Does a β2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S
  • 雑誌名: Nat. Med. 巻: 18 号: 9 ページ: 1324-1325
  • DOI: 10.1038/nm.2809
  • 査読あり
• [雑誌論文] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on theWNK-OSR1/SPAK kinase cascade (2011)
  • 著者名/発表者名: Chiga M, Rafiqi FH, Alessi DR, Sohara E, Ohta A, Rai T, Sasaki S, Uchida S
  • 雑誌名: J. Cell Sci 巻: 124 ページ: 1391-5
  • 査読あり
• [雑誌論文] Impaired phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter by oxidative stress-responsive kinase-1deficiency manifests hypotension and Bartter-like syndrome (2011)
  • 著者名/発表者名: Lin SH, Yu IS, Jiang ST, Lin SW, Chu P, Chen A, Sytwu HK, Sohara E, Uchida S, Sasaki S, Yang SS
  • 雑誌名: Proc. Natl. Acad. Sci. U S A 巻: 108 ページ: 17538-43
  • 査読あり
• [雑誌論文] Uchida S.Regulation of WNK1 kinase by extracellular potassium (2011)
  • 著者名/発表者名: Naito S, Ohta A, Sohara E, Ohta E, RaiT, Sasaki S
  • 雑誌名: Clin. Exp. Nephrol 巻: 15 ページ: 195-202
  • 査読あり
• [雑誌論文] Generation and analyses of R8L barttin knockin mouse (2011)
  • 著者名/発表者名: Nomura N, Tajima M, Sugawara N, Morimoto T, Kondo Y, Ohno M, Uchida K, Mutig K, Bachmann S, Soleimani M, Ohta E, Ohta A, Sohara E, Okado T, Rai T, JentschTJ, Sasaki S, Uchida S
  • 雑誌名: Am. J. Physiol. Renal. Physiol 巻: 301
  • 査読あり
• [雑誌論文] Dexamethasone increases the phosphorylation of nephrin in culturedpodocytes (2011)
  • 著者名/発表者名: Ohashi T, Uchida K, Uchida S, SasakiS, Nitta K
  • 雑誌名: Clin. Exp. Nephrol 巻: 15 ページ: 688-93
  • 査読あり
• [雑誌論文] Immunolocalization of WNK4 in mouse kidney (2011)
  • 著者名/発表者名: Ohno M, Uchida K, Ohashi T, Nitta K, Ohta A, Chiga M, Sasaki S, Uchida S
  • 雑誌名: Histochem. Cell Biol 巻: 136 ページ: 25-35
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCCco-transporters in vivo (2011)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Misawa M, ChigaM, Alessi DR, Sasaki S, Uchida S
  • 雑誌名: Biology Open 巻: 1(2) ページ: 120-7
  • 査読あり
• [雑誌論文] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E, Rai T, Yang SS, Ohta A, NaitoS, Chiga M, Nomura N, Lin SH, VandewalleA, Ohta E, Sasaki S, Uchida S
  • 雑誌名: PLoS One 巻: 6
  • 査読あり
• [雑誌論文] Severe hyperparathyroidism in a pre-dialysis chronic kidney disease patient treated with a very low protein diet (2011)
  • 著者名/発表者名: Ohta E
  • 雑誌名: J. Bone Miner. Metab. 巻: (published online) 号: 2 ページ: 238-242
  • DOI: 10.1007/s00774-011-0320-6
  • 査読あり
• [雑誌論文] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade (2011)
  • 著者名/発表者名: Chiga M
  • 雑誌名: J. Cell Sci. 巻: Vol.124 号: 9 ページ: 1391-5
  • DOI: 10.1242/jcs.084111
  • 査読あり
• [雑誌論文] Impaired phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter by oxidative stress-responsive kinase-1 deficiency manifests hypotension and Bartter-like syndrome (2011)
  • 著者名/発表者名: Lin SH
  • 雑誌名: Proc. Natl. Acad. Sci. USA. 巻: Vol.108 号: 42 ページ: 17538-43
  • DOI: 10.1073/pnas.1107452108
  • 査読あり
• [雑誌論文] Regulation of WNK1 kinase by extracellular potassium (2011)
  • 著者名/発表者名: Naito S
  • 雑誌名: Clin. Exp. Nephrol. 巻: Vol.15 号: 2 ページ: 195-202
  • DOI: 10.1007/s10157-010-0378-9
  • 査読あり
• [雑誌論文] Generation and analyses of R8L barttin knockin mouse. (2011)
  • 著者名/発表者名: Nomura N
  • 雑誌名: Am. J. Physiol. Renal. Physiol. 巻: 301
  • 査読あり
• [雑誌論文] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E
  • 雑誌名: PLoS One 巻: Vol.6 号: 8 ページ: e24277-e24277
  • DOI: 10.1371/journal.pone.0024277
  • 査読あり
• [学会発表] Role of WNK kinases in the blood pressure regulation (2012)
  • 著者名/発表者名: Uchida S
  • 学会等名: Taiwan Society of Nephrology 2012
  • 発表場所: China Medical University, Taichung, Taiwan
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2012)
  • 著者名/発表者名: Uchida S
  • 学会等名: 2012 International Ion Channel Conference
  • 発表場所: The New world of anion and cation channels. Jeju, Korea
• [学会発表] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Naito S, Oi K, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Development of sandwich enzyme linked immunosorbent assay (ELISA) formeasurement of urinary total and phosphorylated Na-Cl cotransporter (NCC) protein (2012)
  • 著者名/発表者名: Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system (2012)
  • 著者名/発表者名: Kikuchi E, Mori T, Isobe K, Sohara E,Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of AmericanSociety of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4 (2012)
  • 著者名/発表者名: Mori T, Sohara E, Rai T, Sasaki ,Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families (2012)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi, EUchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, RaiT, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta (2012)
  • 著者名/発表者名: Zeniya M, Sohara E, Oi K, Chiga M, Susa K, Mori T, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
• [学会発表] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Impaired trafficking of polycystin-1 may be a key mechanism of cyst formation in the aquaporin-11 knockout mouse. (2012)
  • 著者名/発表者名: Inoue Y
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Development of sandwich enzyme linked immunosorbent assay (ELISA) for measurement of urinary total and phosphorylated Na-Clcotransporter (NCC) protein. (2012)
  • 著者名/発表者名: Isobe K
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system. (2012)
  • 著者名/発表者名: Kikuchi E
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4. (2012)
  • 著者名/発表者名: Mori T
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families. (2012)
  • 著者名/発表者名: Sasaki S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta. (2012)
  • 著者名/発表者名: Zeniya M
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin. (2012)
  • 著者名/発表者名: Uchida S
  • 学会等名: 2012 International Ion Channel Conference.
  • 発表場所: the Shilla Hotel
  • 招待講演
• [学会発表] Role of WNK kinases in the blood pressure regulation. (2012)
  • 著者名/発表者名: Uchida S
  • 学会等名: Taiwan Society of Nephrology 2012
  • 発表場所: China Medical University
  • 招待講演
• [学会発表] Generation and analyses of AQP11 BAC transgenic mice (2011)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Development of new systems to measure total and phosphorylated Na-Cl cotransporter(NCC)protein in human urine (2011)
  • 著者名/発表者名: Isobe K, Ohta A, Sohara E, Rai T, SasakiS, Uchida S.
  • 学会等名: The 44th Annual Meeting of AmericanSociety of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Urinary excretion of Na-Cl Cotransporter in exosomes is increased by high salt diet as well as low salt diet (2011)
  • 著者名/発表者名: Kahn MZH, Sohara E, Ohta A, Naito S, Chiga M, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] CFTR is highly expressed in the cyst-lining epithelial cells of the AQP11knockout mouse kidney (2011)
  • 著者名/発表者名: Kobayashi K, Uchida S, Sasaki S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] High throughput screening of drugs that inhibit WNK-OSR1/SPAK signaling cascade (2011)
  • 著者名/発表者名: Mori T, Ohta A, Sohara E, Rai T, SasakiS, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Increased Na-Cl cotransporter phosphorylation in hyperinsulinemic db/db mice is regulated by insulin/PI3K pathway (2011)
  • 著者名/発表者名: Nishida H, Sohara E, Alessi DR, NomuraN, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of AmericanSociety of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2011)
  • 著者名/発表者名: Nomura N, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Renal phenotype of WNK3 knockout mouse (2011)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Chiga M, AlessiDR, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Acute insulin stimulation inducesphosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E, Rai T, Yang SS, Ohta A, Chiga M, Nomura N, Lin SH, Vandewalle A, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] WNK-OSR1/SPAK-SLC12A phosphorylation cascade in the WNK1 (+/-) mice (2011)
  • 著者名/発表者名: Susa K, Ohta A, Sohara E, Rai T, KitaS, Iwamoto T, Alessi DR, Sasaki S, UchidaS
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] Increased protein abundance of the mutant WNK4 may be a cause of the increased WNK4 kinase activity in the mouse model of pseudohypoaldosteronism type II (2011)
  • 著者名/発表者名: Wakabayashi M, Naito S, Sohara E, RaiT, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
• [学会発表] A new signal cascade in kidney regulating blood pressure and ion homeostasis.
  • 著者名/発表者名: Uchida S
  • 学会等名: The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology(招待講演)
  • 発表場所: COEX, Seoul, Korea.
• [図書] 細胞工学 (2012)
  • 著者名/発表者名: 内田信一
• [図書] 脱水腎と透析 (2012)
  • 著者名/発表者名: 笠木祐里,内田信一
• [図書] 日本臨床別冊腎臓症候群(上) (2012)
  • 著者名/発表者名: 高橋大栄,内田信一
• [図書] 遺伝子医学MOOK19トランスポートソーム生体膜輸送機構の全体像に迫る (2011)
  • 著者名/発表者名: 須佐紘一郎,内田信一
• [図書] 医学のあゆみ (2011)
  • 著者名/発表者名: 三澤慕子,内田信一
• [図書] 内分泌・糖尿病・代謝内 (2011)
  • 著者名/発表者名: 若林麻衣,内田信一
• [図書] バソプレシンと病態形成の関わり (2011)
  • 著者名/発表者名: 野村尚弘,内田信一
  • 出版者: メディカルレビュー社
• [図書] トランスポートソームの世界 (2011)
  • 著者名/発表者名: 日比野浩,内田信一,野村尚弘
  • 出版者: 京都廣川書店
• [図書] トランスポートソームの世界. 金井好克/竹島浩/森泰生/久保義弘編著 (2011)
  • 著者名/発表者名: 内田信一, 野村尚弘.
  • 総ページ数: 471
  • 出版者: 京都廣川書店
• [図書] 偽性低アルドステロン症II型
  • 著者名/発表者名: 森崇寧,内田信一
• [備考] 東京医科歯科大学腎臓内科学ホーム
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科学ホームページ
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考]
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm