視神経軸索流のライブイメージングを用いた緑内障の神経保護治療評価の確立

• 研究課題/領域番号: 23659814
• 研究種目: 挑戦的萌芽研究
• 配分区分: 基金
• 研究分野: 眼科学
• 研究機関: 熊本大学
• 研究代表者: 谷原 秀信 熊本大学, 大学院・生命科学研究科, 教授 (60217148)
• 研究期間 (年度): 2011 – 2012
• 研究課題ステータス: 完了 (2012年度)
• 配分額: 3,640千円 (直接経費: 2,800千円、間接経費: 840千円); 2012年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円); 2011年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
• キーワード: 緑内障 / 網膜神経節細胞 / 軸索輸送 / ２光子レーザー顕微鏡

研究概要

2光子レーザー顕微鏡を用いてミトコンドリアが蛍光標識された遺伝子改変マウスの網膜神経節細胞の観察を行った。上強膜静脈をレーザーで焼灼して眼圧の上昇を促し緑内障のモデルとしたところ、網膜神経節細胞が細胞死を起こす前の段階で、網膜神経節細胞軸索内のミトコンドリアの軸索輸送が障害されていることを非侵襲的にリアルタイムに観察できた。 網膜神経節細胞における軸索輸送異常は緑内障病態に関わっていると推測された

研究成果

• [雑誌論文] Long-term outcomes and prognostic factors for trabeculectomy with mitomycin C in eyes with uveitic glaucoma : a retrospective cohort study (2014)
  • 著者名/発表者名: Iwao K, Inatani M, Seto T, Takihara Y, Ogata-Iwao M, Okinami S, Tanihara H
  • 雑誌名: Journal of Glaucoma 巻: 23 号: 2 ページ: 88-94
  • DOI: 10.1097/ijg.0b013e3182685167
  • 査読あり
• [雑誌論文] A prospective comparison between trabeculectomy with mitomycin C and phacotrabeculectomy with mitomycin C (2013)
  • 著者名/発表者名: Ogata-Iwao M, Inatani M, Takihara Y, Inoue T, Iwao K, Tanihara H
  • 雑誌名: Acta Ophthalmologica 巻: 91 号: 6 ページ: 500-501
  • DOI: 10.1111/aos.12133
  • 査読あり
• [雑誌論文] Filtration bleb revision guided by three-dimensional anterior segment optical coherence tomography (2013)
  • 著者名/発表者名: Kojima S, Inoue T, Kawaji T, Tanihara H
  • 雑誌名: Journal of Glaucoma 巻: (in press) 号: 5 ページ: 312-315
  • DOI: 10.1097/ijg.0b013e3182741ee6
  • 査読あり
• [雑誌論文] Risk factors for hyphema after trabeculectomy with mitomycin C (2013)
  • 著者名/発表者名: Kojima S, Inatani M, Shobayashi K, Haga, A, Inoue T, Tanihara H
  • 雑誌名: Journal of Glaucoma 巻: (in press) 号: 5 ページ: 307-311
  • DOI: 10.1097/ijg.0b013e3182741c85
  • 査読あり
• [雑誌論文] Frequency and risk factors for neovascular glaucoma after vitrectomy in eyes with proliferative diabetic retinopathy (2013)
  • 著者名/発表者名: Goto A, Inatani M, Inoue T, Awai-Kasaoka N, Takihara Y, Ito Y, Fukushima M, Tanihara H
  • 雑誌名: Journal of Glaucoma 巻: 22 号: 7 ページ: 572-576
  • DOI: 10.1097/ijg.0b013e31824d514a
  • 査読あり
• [雑誌論文] Japanese Phaco-Goniosynechialysis Multicenter Study Group. Long-term efficacy of goniosynechialysis combined with phacoemulsification for primary angle closure (2013)
  • 著者名/発表者名: Kameda T, Inoue T, Inatani M, Tanihara H
  • 雑誌名: Graefe's Archive for Clinical and Experimental Ophthalmology 巻: 251 号: 3 ページ: 825-830
  • DOI: 10.1007/s00417-012-2091-8
  • 査読あり
• [雑誌論文] Phase 1 Clinical Trials of a Selective Rho Kinase Inhibitor, K-115. (2013)
  • 著者名/発表者名: Tanihara H
  • 雑誌名: JAMA Ophthalmology 巻: 印刷中
  • 査読あり
• [雑誌論文] A prospective comparison between trabeculectomy with mitomycin C and phacotrabeculectomy with mitomycin C. (2013)
  • 著者名/発表者名: Ogata-Iwao M
  • 雑誌名: Acta Ophthalmologica 巻: 印刷中
  • 査読あり
• [雑誌論文] Impact of phacoemulsification on failure of trabeculectomy with mitomycin C (2012)
  • 著者名/発表者名: Awai-Kasaoka N, Inoue T, Takihara Y, Kawaguchi A, Inatani M, 7人5番目
  • 雑誌名: J Cataract Refract Surg 巻: 38 号: 3 ページ: 419-424
  • DOI: 10.1016/j.jcrs.2011.09.035
  • NAID: 120005148223
  • 査読あり
• [雑誌論文] A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma (2012)
  • 著者名/発表者名: Hayashi H, Eguchi Y, Fukuchi-Nakaishi Y, Takeya M, Nakagata N, Tanaka K, Vance JE, Tanihara H
  • 雑誌名: J Biol Chem 巻: 287(30) 号: 30 ページ: 25395-406
  • DOI: 10.1074/jbc.m112.370130
  • 査読あり
• [雑誌論文] The effect of Rho-associated protein kinase inhibitor on monkey Schlemm's canal endothelial cells (2012)
  • 著者名/発表者名: Kameda T, Inoue T, Inatani M, 9人3番目
  • 雑誌名: Invest Ophthalmol Vis Sci 巻: 53 号: 6 ページ: 3092-3103
  • DOI: 10.1167/iovs.11-8018
  • 査読あり
• [雑誌論文] Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor of pseudophakic glaucomatous eyes (2012)
  • 著者名/発表者名: Inoue T, Kawaji T, Inatani M, Kameda T, Yoshimura N, Tanihara H
  • 雑誌名: Journal of Cataract and Refractive Surgery 巻: 38 号: 8 ページ: 1389-1397
  • DOI: 10.1016/j.jcrs.2012.04.028
  • 査読あり
• [雑誌論文] Prognostic risk factors for failure of trabeculectomy with mitomycin C after vitrectomy (2012)
  • 著者名/発表者名: Inoue T, Inatani M, Takihara Y, Awai-Kasaoka N, Ogata-Iwao M, Tanihara H
  • 雑誌名: Japanese Journal of Ophthalmology 巻: 56 号: 5 ページ: 1389-1397
  • DOI: 10.1007/s10384-012-0171-2
  • NAID: 10031039818
  • 査読あり
• [雑誌論文] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on aqueous humor outflow facility (2012)
  • 著者名/発表者名: Tsuboi N, Inoue T, Kawai M, Inoue-Mochita M, Fujimoto T, Awai-Kasaoka N, Yoshida A, Tanihara H
  • 雑誌名: Investigative Ophthalmology & Visual Science 巻: 53 号: 10 ページ: 6702-6707
  • DOI: 10.1167/iovs.12-10376
  • 査読あり
• [雑誌論文] Involvement of RhoA/Rho-associated kinase signal transduction pathway in dexamethasone-induced alterations in aqueous outflow. (2012)
  • 著者名/発表者名: Fujimoto T
  • 雑誌名: Investigative Ophthalmology and Visual Science 巻: 53 号: 11 ページ: 7097-7108
  • DOI: 10.1167/iovs.12-9989
  • 査読あり
• [雑誌論文] Elevated Levels ofMonocyte Chemoattractant Protein-1in the Aqueous Humor afterPhacoemulsification (2012)
  • 著者名/発表者名: Kawai M, Inoue T, Inatani M, TsuboiN, Matsukawa A, Yoshida A,Tanihara H
  • 雑誌名: InvestigativeOphthalmology & Visual Science 巻: 53 ページ: 7951-7960
  • 査読あり
• [雑誌論文] Precise identification of filtration openings on the scleral flap by three-dimensional anterior segment optical coherence tomography (2012)
  • 著者名/発表者名: Inoue T, Matsumura R, Kuroda U, Nakashima KI, Kawaji T, Tanihara H
  • 雑誌名: Investigative Ophthalmology & Visual Science 巻: 53 号: 13 ページ: 8288-8294
  • DOI: 10.1167/iovs.12-10941
  • 査読あり
• [雑誌論文] Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification. (2012)
  • 著者名/発表者名: Kawai M
  • 雑誌名: Investigative Ophthalmology and Visual Science 巻: 53 号: 13 ページ: 7951-7960
  • DOI: 10.1167/iovs.12-10231
  • 査読あり
• [雑誌論文] Trabeculectomy with mitomycin C for open-angle glaucoma in phakic eyes vs pseudophakic eyes after phacoemulsification (2011)
  • 著者名/発表者名: Takihara Y, Inatani M, 8人2番目
  • 雑誌名: Arch Ophthalmol 巻: 129 号: 2 ページ: 152-157
  • DOI: 10.1001/archophthalmol.2010.348
  • 査読あり
• [雑誌論文] Dynamic imaging of axonal transport in living retinal ganglion cells in vitro. (2011)
  • 著者名/発表者名: Takihara Y, Inatani M, Hayashi H, Adachi N, Iwao K, Inoue T, Iwao M, Tanihara H.
  • 雑誌名: Invest Ophthalmol Vis Sci. 巻: 52 号: 6 ページ: 3039-3945
  • DOI: 10.1167/iovs.10-6435
  • 査読あり
• [雑誌論文] Dynamic imaging of axonal transport in living retinal ganglion cells in vitro. (2011)
  • 著者名/発表者名: Takihara Y, Inatani M, Hayashi H, Adachi N, Iwao K, Inoue T, Iwao M, Tanihara H.
  • 雑誌名: Investigative Ophthalmology & Visual Science 巻: 52 ページ: 3039-3045
  • 査読あり
• [雑誌論文] Trabeculectomy with mitomycin for open-angle glaucoma in phakic vs pseudophakic eyes after phacoemulsification. (2011)
  • 著者名/発表者名: Takihara Y, Inatani M, Seto T, Iwao K, Iwao M, Inoue T, Kasaoka N, Murakami A, Futa R, Tanihara H.
  • 雑誌名: Archives of Ophthalmology 巻: 129 ページ: 152-157
  • 査読あり
• [雑誌論文] Combined intravitreal bevacizumab and trabeculectomy with mitomycin C versus trabeculectomy with mitomycin C alone for neovascular glaucoma. (2011)
  • 著者名/発表者名: Takihara Y, Inatani M, Kawaji T, Fukushima M, Iwao K, Iwao M, Tanihara H.
  • 雑誌名: Journal of Glaucoma 巻: 20 ページ: 196-201
  • 査読あり
• [学会発表] 緑内障治療の新しい展開を目指して (2012)
  • 著者名/発表者名: 谷原秀信
  • 学会等名: 第66回日本臨床眼科学会
  • 発表場所: 京都国際会議場
• [学会発表] PinpointIdentification of filtration openingon the scleral flap margins created bytrabeculectomy (2012)
  • 著者名/発表者名: Inoue T, Kawaji T, Matsumura R, KurodaU, Nakashima K, Tanihara H
  • 学会等名: European Glaucoma Society 2012
  • 発表場所: Copenhagen, Denmark
• [学会発表] Involvement of RhoA/Rho-associatedkinase signal transduction pathway inthe dexamethasone-inducedalterations in aqueous outflow (2012)
  • 著者名/発表者名: Fujimoto T, Inoue T, Inoue-Mochita M,Kameda T, Kasaoka N, Tanihara H
  • 学会等名: European Glaucoma Society 2012
  • 発表場所: Copenhagen, Denmark
• [学会発表] The Effect of MonocyteChemoattractant Protein-1/CCChemokine Ligand 2 on The AqueousHumor Outflow Facility (2012)
  • 著者名/発表者名: Tsuboi N, Inoue T, Fujimoto T,Inoue-Mochita M, Kasaoka N, Kawai M,Shobayashi K, Yoshida A, Tanihara H
  • 学会等名: European Glaucoma Society 2012
  • 発表場所: Copenhagen, Denmark
• [学会発表] Filtration bleb-associated tearfilm sign demonstrated by threedimensional anterior segment opticalcoherent tomography (2012)
  • 著者名/発表者名: Kojima S, Inoue T, Kawaji T, TaniharaH.
  • 学会等名: European Glaucoma Society 2012
  • 発表場所: Copenhagen, Denmark
• [学会発表] Multiplex Immunoassayof Aqueous Humor in CataractPatients: Impact ofPhacoemulsification (2012)
  • 著者名/発表者名: Kawai M,Inoue T, Inatani M, Tsuboi N, YoshidaA, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] PinpointIdentification of Filtration Openingon the Scleral Flap Margins Created byTrabeculectomy (2012)
  • 著者名/発表者名: Tanihara H,Inoue T, Kawaji T, Matsumura R, KurodaU, Nakashima K.
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] Hyaluronic Acid as aContributing Factor toEpithelial-Mesenchymal Transition inRetinal Pigment Epithelial Cells andAngiogenesis (2012)
  • 著者名/発表者名: TakahashiE, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] DiagnosticUsefulness of Indocyanine GreenFundus Angiography forTransthyretin-related FamilialAmyloidotic Polyneuropathy (2012)
  • 著者名/発表者名: Kawaji T,Hara R, Eiki D, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] The Effects ofRho/ROCK Activation inDexamethasone-Induced Increase ofAqueous-Outflow Resistance (2012)
  • 著者名/発表者名: Fujimoto T,Inoue T, Inoue-Mochita M, Kameda T,Kasaoka N, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] The Effect of Monocyte ChemoattractantProtein-1/CC Chemokine Ligand 2 on the Aqueous Humor Outflow Facility (2012)
  • 著者名/発表者名: Tsuboi N,Inoue T, Fujimoto T, Inoue-Mochita M,Kasaoka N, Kawai M, Shobayashi K,Yoshida A, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] Angptl2 KnockoutMice Suppress Inflammation In RetinalVessels Induced By A High-fat Diet (2012)
  • 著者名/発表者名: Ito Y,Oike Y, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] A ProspectiveStudy of Phakic vs Pseudophakic EyesAfter Phacoemulsification inTrabeculectomy for Open-AngleGlaucoma (2012)
  • 著者名/発表者名: Takihara Y,Inatani M, Iwao M, Kawai M, Inoue T,Iwao K, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] Filtration Bleb-associated Tear FilmSign Demonstrated by ThreeDimensional Anterior Segment Optical Coherence Tomography (3D AS-OCT) (2012)
  • 著者名/発表者名: Kojima S,Inoue T, Kawaji T, Kuroda U, NakashimaK, Matsumura R, Tanihara H
  • 学会等名: The Annual Meeting of the Associationfor Research in Vision andOphthalmology
  • 発表場所: FortLauderdale, Florida, USA
• [学会発表] 開放隅角緑内障の線維柱帯切除術:有水晶体眼と超音波乳化吸引術既往眼との前向き比較 (2012)
  • 著者名/発表者名: 瀧原祐史, 稲谷大, 岩尾美奈子, 川井基史, 井上俊洋, 岩尾圭一郎, 谷原秀信
  • 学会等名: 第116回日本眼科学会総会
  • 発表場所: 東京国際フォーラム
• [学会発表] Filtration bleb revision guided by three-dimensional anterior segmental optical coherence tomography. (2012)
  • 著者名/発表者名: Kojima S
  • 学会等名: The 27th APAO Congress
  • 発表場所: Pusan, Korea
• [学会発表] Experimental study on signaling pathways activated by scraping and exposure to vitreous samples in ARPE-19 cells. (2012)
  • 著者名/発表者名: Takahashi E
  • 学会等名: The 27th APAO Congress
  • 発表場所: Pusan, Korea
• [学会発表] Multiplex Immunoassay of Aqueous Humor in Cataract Patients: Impact of Phacoemulsification. (2012)
  • 著者名/発表者名: Kawai M
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] Pinpoint Identification of Filtration Opening on the Scleral Flap Margins Created by Trabeculectomy. (2012)
  • 著者名/発表者名: Tanihara H
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] Hyaluronic Acid as a Contributing Factor to Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells and Angiogenesis. (2012)
  • 著者名/発表者名: Takahashi E
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] Diagnostic Usefulness of Indocyanine Green Fundus Angiography for Transthyretin-related Familial Amyloidotic Polyneuropathy. (2012)
  • 著者名/発表者名: Kawaji T
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] The Effects of Rho/ROCK Activation in Dexamethasone-Induced Increase of Aqueous-Outflow Resistance. (2012)
  • 著者名/発表者名: Fujimoto T
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] The Effect of Monocyte Chemoattractant Protein-1/CC Chemokine Ligand 2 on The Aqueous Humor Outflow Facility (2012)
  • 著者名/発表者名: Tsuboi N
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] Angptl2 Knockout Mice Suppress Inflammation In Retinal Vessels Induced By A High-fat Diet. (2012)
  • 著者名/発表者名: Ito Y
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] A Prospective Study of Phakic vs Pseudophakic Eyes After Phacoemulsification in Trabeculectomy for Open-Angle Glaucoma. (2012)
  • 著者名/発表者名: Takihara Y
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] Filtration Bleb-associated Tear Film Sign Demonstrated by Three Dimensional Anterior Segment Optical Coherence Tomography (3D AS-OCT). (2012)
  • 著者名/発表者名: Kojima S
  • 学会等名: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • 発表場所: Fort Lauderdale, Florida, USA
• [学会発表] 発達緑内障に対する緑内障手術の治療成績 (2011)
  • 著者名/発表者名: 笠岡奈々子, 井上俊洋, 稲谷大, 瀧原祐史, 岩尾美奈子, 正林耕平, 川井基史, 坪井尚子, 谷原秀信
  • 学会等名: 第65回日本臨床眼科学会
  • 発表場所: 東京国際フォーラム
• [学会発表] 開放隅角緑内障における房水内 MCP-1 濃度が線維柱帯切除術の手術成績に与える影響 (2011)
  • 著者名/発表者名: 井上俊洋, 稲谷大, 亀田隆範, 吉村長久,谷原秀信
  • 学会等名: 第22回日本緑内障学会
  • 発表場所: 秋田アトリオン
• [学会発表] 開放隅角緑内障に対するトラベクレクトミー単独手術と超音波水晶体乳化吸引術同時手術との前向き比較研究 (2011)
  • 著者名/発表者名: 稲谷大, 岩尾美奈子, 瀧原祐史, 井上俊洋,岩尾圭一郎, 谷原秀信
  • 学会等名: 第22回日本緑内障学会
  • 発表場所: 秋田アトリオン
• [学会発表] The Effect of AqueousMonocyte Chemotactic Protein-1Concentration on the Short-termFailure of Trabeculectomy withMitomycin C in Eyes with Open-angleGlaucoma. (2011)
  • 著者名/発表者名: InoueT, Inatani M, Kameda T, Yoshimura N,Tanihara H
  • 学会等名: World Glaucoma Congress 2011
  • 発表場所: Paris, France
• [学会発表] The Effects of Rho/ROCK Activation inDexamethasone Induced Increase ofAqueous Outflow Resistance (2011)
  • 著者名/発表者名: Fujimoto T, Inoue T, Inoue-Mochita M,Kameda T, Kasaoka N, Tanihara H
  • 学会等名: World Glaucoma Congress 2011
  • 発表場所: Paris, France
• [学会発表] 培養網膜神経節細胞軸索流の動的画像解析 (2011)
  • 著者名/発表者名: 瀧原祐史, 稲谷大, 林秀樹, 安達直樹, 岩尾圭一郎, 井上俊洋, 岩尾美奈子, 谷原秀信
  • 学会等名: 日本眼科学会総会
  • 発表場所: 東京国際フォーラム、東京
• [備考] ホームページ等, 熊本大学大学院生命科学研究部眼科学分野研究業績
  • URL: http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html
• [備考] 熊本大学大学院生命科学研究部眼科学分野 研究業績
  • URL: http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html