| 研究課題/領域番号 |
23K08893
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分56040:産婦人科学関連
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| 研究機関 | 京都府立医科大学 |
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研究代表者 |
カーン カレク 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60336162)
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| 研究分担者 |
森 泰輔 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00569824)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2024年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
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| キーワード | adenomyosis / uterine infection / inflammation / chronic endometritis / bacterial colonization / molecular method |
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| 研究開始時の研究の概要 |
Increased intrauterine microbial colonization and occurrence of chronic endometriosis has been demonstrated previously from our laboratory. However, information on this issue in different types and subtypes of adenomyosis is still unknown. Here we plan to investigate intrauterine microbial colonization and occurrence of chronic endometriosis in women with focal and diffuse adenomyosis with and without previous treatment with GnRHa and also in women with intrinsic and extrinsic adenomyosis.
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| 研究実績の概要 |
We evaluated microbial profiles in the endometrial samples that were collected from women with different types of adenomyosis and in women without adenomyosis. We also examined inflammatory reaction and micro-vessel density in the collected tissue samples collected from these women.
In our initial findings with 16SrDNA metagenome analysis with the endometrial samples, we found that a proportion of major microbial genera has a higher tendency in women with intrinsic adenomyosis (n=8) than that in extrinsic adenomyosis (n=6). These findings were coincided with an increased tissue infiltration of CD68-stained macrophages and CD31-stained micro-vessel density. Further analysis with increased collection of samples in each group may confirm the validity of these findings.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We are still collecting additional samples from each group of intrinsic and extrinsic adenoymosis and in control women without adenomyosis with a target of 20 samples in each group.
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| 今後の研究の推進方策 |
1. Collection of additional samples from eutopic and ectopic endometria of women with intrinsic and extrinsic adenomyosis.
2. Perform 16S rDNA metagenome analysis to examine the pattern of different microbial profile in each group of sample.
3. Immunohistochemical analysis with antibodies against CD68, Ki-67 and CD31 in each group of tissue samples.
4. Immunohisotchemical analysis with antibody against CD138 to examines the pattern of plasma cell infiltration in each group of tissue samples.
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