| 研究課題/領域番号 |
23K10910
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分59040:栄養学および健康科学関連
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| 研究機関 | 早稲田大学 |
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研究代表者 |
馬 思慧 早稲田大学, 人間総合研究センター, 次席研究員 (70974343)
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| 研究分担者 |
賈 慧娟 東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (60456324)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2024年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | aging / crosstalk / skeletal muscle / adipose / 老化 / クロストーク |
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| 研究開始時の研究の概要 |
Deciphering how senescent cells and tissue deliver the signals and messages, are at the nexus of mechanisms involved in longevity and age-related metabolic dysfunction. Multi-tissue/organ crosstalk, defined as the mutual biological communication between distant tissue or organs mediated by signaling factors, may transmit aging signals initiated by the early-senescent cell/tissue/organs, spreading the aging signal. The present study aims to discuss how aging signals are conveyed by senescent cells and organs, to other cells and organs, as well as the underlying mechanisms.
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| 研究実績の概要 |
This year, we successfully induced cellular senescence in muscle cells through serial passaging and detected changes in the senescence-associated secretory phenotype and related mRNA expression. The detection of senescent cells was also achieved. Additionally, we successfully isolated extracellular vesicles from the senescent cells.Our research aims to further understand the role of senescent cell-derived EVs in the aging process and their potential impact on surrounding tissues. By studying the effects of these EVs on young cells, we hope to gain insights into the mechanisms of cellular senescence and its contribution to age-related pathologies.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Due to my transfer to a new university, there has been a slight delay in the progress of some parts of the research plan. However, in the new academic year, I will make every effort to catch up and get the project back on track as quickly as possible. I am confident that with the support of my new institution and colleagues, I will be able to overcome any temporary setbacks and continue to advance this important research.
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| 今後の研究の推進方策 |
今後の研究の推進方策 Plans for the Research Scheme:
In the new academic year, we plan to investigate the role of intercellular communication in the progression of cellular senescence by co-culturing various combinations of senescent and young cells. This will include senescent cell-young cell and young cell-senescent cell co-cultures.
To comprehensively detect gene expression changes, we will employ RNA-sequencing (RNA-seq) technology. This approach will allow us to identify key genes and pathways involved in the senescence process and the effects of intercellular communication on senescence progression.
Furthermore, we will combine these findings with genetic engineering techniques to observe the specific influence of key genes on the senescence process. By manipulating the expression of these genes, we aim to gain a deeper understanding of their roles in regulating cellular senescence and the impact of intercellular communication on this process.
Through these multifaceted approaches, we hope to unravel the complex mechanisms underlying cellular senescence and the significance of intercellular communication in the aging process. The insights gained from this research may contribute to the development of novel strategies for managing age-related diseases and promoting healthy aging.
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