| 研究課題/領域番号 |
23K11509
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分64040:自然共生システム関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
MIRYEGANEH Matin 沖縄科学技術大学院大学, 統合群集生態学ユニット, スタッフサイエンティスト (90793505)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2025年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2024年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
2023年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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| キーワード | Mangroves / epigenetics / genomic / climate change |
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| 研究開始時の研究の概要 |
This research uses stress tolerant mangrove trees as experimental model to study the role of epigenetic changes in responses to environmental stresses that are predicted by climate change. This proposed research will pair ecological methods in natural environments with genomic and epigenomic approaches in laboratories to provide comprehensive understandings of gene functions and adaptations of plants under stress. Additionally, it investigates whether and how epigenetic modification of the genome allow plants to respond to environmental stress.
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| 研究実績の概要 |
Since the beginning of my Kakenhi project (Apr. 2023) I have successfully extracted the DNA from 2 other mangrove species (Rhizopphora stylosa, Kandelia obovata) and sequenced the genome, using PacBio-Hifi long read sequencing. I then de-novo assembled and annotated the genome. As for Hi-C sequencing, the 3 species of mangroves (Bruguiera gymnorhiza, Rhizopphora stylosa, Kandelia obovata) are being processed and library preparation is under preparation. These annotated genomes are the prerequisite of the downstream epigenetic analyses.
In order to study the epigenetic stress memory, I have also done the DNA extraction and whole genome EM-seq sequencing for 96 samples (48 from Bruguiera gymnorhiza) and (48 from kandelia obovata). I have analyzed the data for the 48 samples from B. gymnorhiza and the results are very exciting. I also have extracted the RNA from the same samples and have sequenced them.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Everything is going as planned.
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| 今後の研究の推進方策 |
Next I will analyze the data to investigate the association between expression of the genes and methylated regions of DNA.
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