| 研究課題/領域番号 |
23K13291
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分20020:ロボティクスおよび知能機械システム関連
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| 研究機関 | 名古屋大学 |
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研究代表者 |
金 恩恵 名古屋大学, 未来社会創造機構, 研究員 (30972517)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
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| キーワード | Micromanipulation / Magntic tweezer / Vascular network / Liver tissue / Magnetic field / 3D cellular structure / 3D vascular network / tissue engineering |
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| 研究開始時の研究の概要 |
I will construct 3D hierarchical vascular networks in centimeter-sized live tissues in vitro using magnetic fields. The target size is a liver having 13cm of diameter and hierarchical vessels from portal vein to hepatic sinusoids.
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| 研究実績の概要 |
In the first year, we designed magnetic tweezers for constructing 3D hierarchical vascular networks in a cm-sized liver tissue. In addition, we fabricated several patterns of vascular network in cellular structure in order to compare the efficiency of the vascular networks. For this, we fabricated several patterns of vascular networks; without vascular network, with vertical channel, and with 3D hierarchical channels. After 3 weeks, the cell viability of four samples were analyzed and we found that the tissue having 3D hierarchical channels show higher cell viability than others. From the result, we can see that the fabricated 3D hierarchical channels can supply the nutrient and oxygen to the cells in tissues and improve the cell condition after a long-term culture..
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
In accordance with the original research plan, we designed the magnetic tweezers and verified the efficiency of channel networks by comparing tissue haing several patterns of vascular networks.
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| 今後の研究の推進方策 |
In the next step, we will apply perfusable network within 3D cellular structure by adding circulation system and then to transform from the empty channels to vascular network, I will seed human umbilical vein endothelial cells (HUVECs) to the channels and perfuse the culture medium to the vascular networks. Finally, we will analyze cell condition and liver function of constructed tissue.
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