| 研究課題/領域番号 |
23K13291
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分20020:ロボティクスおよび知能機械システム関連
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| 研究機関 | 名古屋大学 |
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研究代表者 |
金 恩恵 名古屋大学, 未来社会創造機構, 研究員 (30972517)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
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| キーワード | Vascular Network / Micromanipulation / 3D channel / Magntic tweezer / Vascular network / Liver tissue / Magnetic field / 3D cellular structure / 3D vascular network / tissue engineering |
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| 研究開始時の研究の概要 |
I will construct 3D hierarchical vascular networks in centimeter-sized live tissues in vitro using magnetic fields. The target size is a liver having 13cm of diameter and hierarchical vessels from portal vein to hepatic sinusoids.
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| 研究実績の概要 |
In the second year, we further advanced our magnetic tweezer-based system to fabricate more complex and functional 3D hierarchical vascular networks in cm-scale tissue constructs. To evaluate the biological effects of vascularization, we co-cultured HUVECs with liver parenchymal cells (RLC-18 cell line) and created four types of vascular patterns: (1) no vascular network, (2) vertical channel, (3) 3D hierarchical channel, and (4) 3D hierarchical channel with active perfusion. After three weeks of culture, we quantitatively analyzed cell proliferation and viability. Although the current dataset is still limited due to the complexity of co-culture conditions, results showed that the 3D hierarchical channels, particularly with perfusion, promoted higher cell viability and better maintenance of tissue structure compared to other patterns. In addition, we refined the magnetic tweezer design to enable the construction of more diverse and stable channel architectures. Moving forward, we aim to perform additional experiments to increase data volume, detect statistically significant differences, and further validate the effectiveness of hierarchical vascularization for long-term tissue culture.
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| 現在までの達成度 |
現在までの達成度
3: やや遅れている
理由
Due to the complexity of co-culturing two distinct cell types, fine-tuning the culture conditions has posed significant challenges, leading to a relatively limited dataset at this stage. Continuous efforts are being made to accumulate more replicates and improve experimental consistency. Further investigations are essential to achieve statistically robust results and to validate the impact of hierarchical vascularization on tissue viability and function over extended culture periods.
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| 今後の研究の推進方策 |
As the next step, we aim to fabricate a wider variety of vascular channel patterns using the improved magnetic tweezer system. In parallel, we will conduct more extensive biological experiments to increase the dataset and identify statistically significant differences across experimental conditions. This will allow us to further validate the effectiveness of hierarchical vascularization in supporting long-term cell viability and tissue functionality.
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