| 研究課題/領域番号 |
23K13776
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分34020:分析化学関連
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| 研究機関 | 九州大学 |
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研究代表者 |
LIU CHENCHEN 九州大学, 理学研究院, 助教 (50963211)
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| 研究期間 (年度) |
2023-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2024年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2023年度: 2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
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| キーワード | sample preconcentration / single cell analysis / electrophoresis / mass spectroscopy / Molecularly imprinting / Subcellular analysis / Mitochondrial profiling / Electrophoresis / Mass spectrometry |
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| 研究開始時の研究の概要 |
For single cell mitochondria analysis, we propose to develop a microneedle that can be coupled with a mass spectrometer. We plan to investigate the microneedle's ability to extract subcellular mitochondria, separate biomolecules, and perform mass spectrometry analysis of mitochondrial compositions.
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| 研究実績の概要 |
This project aims to devise a technique for analyzing mitochondria at the single-cell level. This approach consists of a highly sensitive sample detection method and a novel material tailored for the collection of mitochondria. Currently, we have successfully developed a highly sensitive capillary electrophoresis method that offers 40 times improved detection compared to state-of-the-art technologies, specifically designed for profiling glycans. We intend to further develop and apply this analysis method for profiling cell samples.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
To profile the composition of mitochondria at the single-cell level, it is necessary to detect trace samples, collect mitochondria, and recover biomolecules. Overcoming the challenges related to specific organelle recognition and analyte preconcentration is crucial. We have made significant progress in achieving sensitive preconcentration and detection of standard glycan samples, and the study is advancing smoothly.
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| 今後の研究の推進方策 |
To achieve sensitive cellular analysis, our plan is to: (1) further investigate sensitive glycan detection and apply it to single-cell analysis, (2) develop new materials and technologies for the specific collection of cellular mitochondria, and (3) design a device platform for trace biomolecule detection. Furthermore, our objective is to develop a device for subcellular composition profiling in an integrated manner, aiming to prevent sample loss and ensure sensitivity. Ultimately, we aim to propose a proof-of-concept method for subcellular analysis as our final goal.
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