| 研究課題/領域番号 |
23K13851
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分37030:ケミカルバイオロジー関連
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| 研究機関 | 北海道大学 |
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研究代表者 |
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2025年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2024年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2023年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
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| キーワード | Fluorescence imaging / Organic synthesis / Breast cancer / Chemical biology / Molecular probe |
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| 研究開始時の研究の概要 |
Non-invasive fluorescence imaging is the most promising method to study the biological phenomenon in the living system. Early stage of breast cancer less than 1 cm size can be cured without fail by surgical resection upon diagnosis by molecular imaging of breast cancer.
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| 研究実績の概要 |
The purpose of our research is to visualize deep living tissue through fluorescence imaging because fluorescence guided cancer surgery is very important for complete removal of solid tumors. So far visible and near-infrared (NIR) region as been used for clinical applications. For deep tissue visualization, shortwave-infrared (SWIR, >1000 nm) emitting fluorophores are important. I proposed to synthesize water soluble, biocompatible, and SWIR emitting organic fluorophores for deep tissue imaging of breast cancer. The intermediate compounds to synthesize proposed organic probe was unstable so, we alternatively developed organic probes which are highly efficient for the proposed biological applications.
We demonstrate that these probes are biocompatible and versatile, which can be conjugated to any antibodies and proteins. Using newly synthesized probes, we carried out in-vivo imaging of breast cancer. Also we demonstrate that these probes can be applicable for multicolor imaging of cancers and blood vessels. Currently, we are working on the development of clinically applicable probes with bright fluorescence for breast cancer imaging. The results obtained from this work is recently published in ACS Applied Materials and Interfaces 2024, 16, 14, 17253-17266.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our aim was to develop biocompatible organic probes for deep tissue imaging of breast cancer. In the first year of the project period, we could synthesize biocompatible fluorescent probes and demonstrate in-vivo imaging of breast cancer. By using the information obtained so far we are proceeding further to develop more stable and clinically applicable probes for breast cancer imaging. The results obtained until now make this project proceed smoothly.
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| 今後の研究の推進方策 |
Some of the intermediate compounds needed to synthesize the proposed organic probe was very low so, we could synthesized alternate probes which are efficient for fluorescence imaging in mice experiments. The water solubility of the developed probes are relatively low, it limits these probes from the clinical application in human. In future we will be carrying out experiments to enhance their water solubility and perform cancer imaging animal model.
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