研究課題/領域番号 |
23K14201
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分44020:発生生物学関連
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研究機関 | 国立研究開発法人理化学研究所 |
研究代表者 |
Trush Olena 国立研究開発法人理化学研究所, 生命機能科学研究センター, 基礎科学特別研究員 (20869143)
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研究期間 (年度) |
2023-04-01 – 2024-03-31
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研究課題ステータス |
完了 (2023年度)
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配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
2023年度: 2,600千円 (直接経費: 2,000千円、間接経費: 600千円)
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キーワード | kidney / nephron / S-shaped body / development / organoid |
研究開始時の研究の概要 |
As an outline, I hope to reveal the regulatory mechanism for nephron morphogenesis at S-shaped body stage. This finding will get immediate application in the generation of improved kidney organoids to achieve better morphology of nephrons in vitro and reduce heterogeneity of kidney organoids.
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研究実績の概要 |
In this project we revealed that Wnt4 ligand is crucial for the formation S-shaped body(SSB)- an important morphological feature of early nephron. We also found that the induction of nephrogenesis by artificial Wnt activator (CHIR99021) causes abnormal branching of the nephron on the edge of distal and proximal region, and, as a result, formation of T-shaped bodies instead of SSB. The branching areas were characterized by the expanded expression of Jag1 ligand and extensive expansion of the distal part of the nephron, and formation of multiple proximal tubeless withing single nephron. We found that medium supplement with human recombinant Wnt4 ligand or Notch inhibitors could partially rescue the phenotype.
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