| 研究課題/領域番号 |
23K14346
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分47030:薬系衛生および生物化学関連
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| 研究機関 | 大阪大学 |
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研究代表者 |
ZWAMA MARTIJN 大阪大学, 産業科学研究所, 特任准教授(常勤) (40827052)
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| 研究期間 (年度) |
2023-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 3,250千円 (直接経費: 2,500千円、間接経費: 750千円)
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| キーワード | RND / Transporter / Multidrug Resistance / MDR / Efflux Pump / Pathogens |
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| 研究開始時の研究の概要 |
Summary: Study RND-type efflux pumps in A. baumannii and P. aeruginosa.
Outline: Investigate three efflux pump complexes in A. baumannii, test inhibitors, sequence for substitutions, and create knockouts and chimeras. Compare P. aeruginosa's MexD and MexF pumps, create chimera proteins, and express pumps in knockouts to test MIC spectra, inhibition, and stability. Create AdeABC, AdeFGH, and AdeIJK knockouts for Japanese isolates. Transform plasmids to compare substrate specificity and inhibitors. Perform MIC experiments and export assays with efflux pump inhibitors.
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| 研究実績の概要 |
One of the leading causes of clinical multidrug-resistant (MDR) bacterial hospital isolates is the over-expression of multidrug efflux pumps belonging to the Resistance-Nodulation-cell Division (RND) protein superfamily. We aimed to understand the roles of efflux pumps in clinical isolates to MDR and also study less-studied clinically relevant pumps. During the first fiscal year of this research project, we started the first cloning of RND-type efflux pumps for this project and created active pumps. The results show interesting, novel findings that are relevant and important to the field of multidrug resistance. The preliminary results were very interesting, and we are currently continuing to investigate the phenotypes to help us understand multidrug resistance caused by the RND-type efflux pumps of P. aeruginosa, E. coli, and A. baumannii. Gene expression analysis was also performed, which gave us detailed information about the resistance factors in the resistant bacteria. In this fiscal year, we were also able to publish three papers, including drafting multiple manuscripts and laying the groundwork for additional research and publications in the near future, likely for the next fiscal year.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The research went smoothly because there were no problems with cloning, and experimental results showed interesting preliminary results already.
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| 今後の研究の推進方策 |
Continue with the research as planned and perform experiments based on the positive procedures and results.
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