| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2024年度: 2,340千円 (直接経費: 1,800千円、間接経費: 540千円)
2023年度: 2,340千円 (直接経費: 1,800千円、間接経費: 540千円)
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| 研究開始時の研究の概要 |
The major seafood-borne pathogen Vibrio parahaemolyticus senses bile as a chemical cue in the intestine to activate the T3SS2, which is responsible for its enterotoxicity but represses the T3SS1 activity. In this study, the applicant tries to understand how bile affects T3SS1 repression. To this end, the responsible molecule will be identified and characterized using biochemistry and molecular biology techniques to understand this regulatory mechanism.
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| 研究実績の概要 |
Pathogenic Vibrio parahaemolyticus causes septicemia and acute gastroenteritis in humans, by utilizing nano weapons called Type 3 Secretion Systems (T3SSs). This bacterium encoded two T3SSs called T3SS1 and T3SS2, whose expression is dictated by certain signals from the surrounding environment. It has been reported that bile, a compound found in the human intestine, acts as a chemical cue to trigger T3SS2 expression while also shutting off T3SS1 expression. In this study, the applicant tries to understand how bile affects T3SS1 repression.
During the first year, the applicant identified conjugated secondary bile acids, which are human metabolites found within bile, as a signal that represses T3SS1. The applicant investigated potential regulators that could serve as a signal for these molecules by examining the gene and protein expression of those regulator mutants in the context of T3SS1. As a result, one regulator (regulator X) is a key factor in this phenomenon. Furthermore, using the transcriptomic analysis, many genes were differentially expressed by regulator X besides T3SS1 downregulation. However, careful examination revealed that regulator X indirectly repressed the T3SS1. To find the direct repressor, the applicant created an expression library of genes activated by regulator X for screening and found genes A, B, and C linked to T3SS1 activity.
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