| 研究課題/領域番号 |
23K15608
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分55060:救急医学関連
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| 研究機関 | 弘前大学 |
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研究代表者 |
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2024年度: 2,210千円 (直接経費: 1,700千円、間接経費: 510千円)
2023年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
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| キーワード | biodosimetry / dicentric / radiation / c-banding / cytogentic / centromere |
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| 研究開始時の研究の概要 |
Accurate and fast radiation dose estimation using cytogenetic biodosimetry is crucial in accidental and occupational exposures to ionizing radiation. To improve this, we've developed a new staining approach using solid stained dicentric chromosomes and a centromere highlighting technique.
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| 研究実績の概要 |
We have combined the conventional solid chromosome staining with a specialized technique that targets and marks the centromeric region using a proteolytic enzyme, acetic saline, and Giemsa. We have reduced the staining to be completed within just ninety minutes. New modifications also minimize chromosome expansion while preserving morphology. This improvement enhanced the identification of chromosomal aberrations. Preliminary results show a significant reduction in dicentric yield measurement disparities among scorers, with the coefficient of variation decreasing from 12% with conventional Giemsa staining to 1.2%. We have begun to modify the technique for use in other laboratories, such as those in South Korea and Singapore. Results have also been shared at international conferences.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our research is progressing smoothly in the laboratory. We have begun sharing our technique with other laboratories and at international conferences. However, we encountered an issue where some chemicals used in the experiment are available in Japan but not in other countries. To address this, we will collaborate with partner laboratories to modify the technique, ensuring it is accessible to all. We will also continue presenting our new staining method at international conferences and performing comparative analyses to validate its effectiveness.
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| 今後の研究の推進方策 |
We plan to continue with manuscript preparation and publish our findings as soon as possible. Following publication, we will conduct intercomparison exercises using the new technique. These exercises will validate the method's effectiveness in reducing variation in chromosomal aberration scoring. By comparing results across different laboratories, we aim to demonstrate the consistency and reliability of our technique, further establishing its usefulness in the scientific community. Additionally, we will share this at a large international conference in the U.S., which will allow other laboratories to use the published results.
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