| 研究課題/領域番号 |
23K16005
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分57030:保存治療系歯学関連
|
|---|
| 研究機関 | 北海道医療大学 |
|---|
研究代表者 |
|
|---|
| 研究期間 (年度) |
2023-04-01 – 2026-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
|
|---|
| キーワード | enamel matrix derivative / ankylosis / periodontal tissue / bone regenaration / Fibroblast / cell proliferation / transplantation / regeneration / trauma |
|---|
| 研究開始時の研究の概要 |
Our recent research has found that enamel matrix derivative- Emdogain (EMD) promotes periodontal tissue regeneration and prevents ankylosis. Basic fibroblast growth factor (bFGF) are promising for regenerating natural tooth structures and tissues, but there has not been much research on bFGF for preventing ankyloses using transplantation method. The main objective of this study is to determine how bFGF and EMD can help regenerate and reconstruct degraded periodontal tissue to prevent ankylosis and root resorption after tooth transplantation.
|
| 研究実績の概要 |
In vivo experiments, investigated the effects of enamel matrix derivative Emdogain (EMD) on the degraded periodontal tissue after immersing the tooth in different tooth storage media.
Male SD rats had their maxillary first molar teeth extracted and immersed in different tooth storage media. They were then transplanted into the abdominal wall, with one group as the control receiving only the tooth and another group receiving EMD application before transplantation.
When EMD was applied before transplantation, it reduced the number of CD68-positive and cathepsin-K positive cells in the PDL. The EMD-treated group also showed increased alveolar bone formation compared to teeth immersed in tooth storage media only.
These findings suggest that EMD promotes PDL fibroblast growth while inhibiting epithelial cell growth, facilitating the regeneration of damaged periodontal tissues.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Since this study follows on from previous research, things are moving along smoothly without any major problems.
|
| 今後の研究の推進方策 |
The transplantation procedure will be followed again in future experiments using different tooth storage media.
Afterwards, histological examinations will be carried out on subcutaneously transplanted rat molars to determine whether Basic Fibroblast Growth Factor and Enamel Matrix Derivative impact the regeneration of periodontal tissues. A variety of storage media will be examined in order to determine the particular causes of ankylosis.
Future studies will examine the regeneration and reconstruction of periodontal ligaments and the amount of alveolar bone formation in different groups will be evaluated using a transplantation model.
|
