| 研究課題/領域番号 |
23K16013
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分57030:保存治療系歯学関連
|
|---|
| 研究機関 | 北海道大学 |
|---|
研究代表者 |
劉 雲青 北海道大学, 歯学研究院, 博士研究員 (50962320)
|
|---|
| 研究期間 (年度) |
2023-04-01 – 2026-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
|
|---|
| キーワード | direct pulp capping / calcium hydroxide / platinum nanoparticles / angiogenesis / inflammatory / polyphosphoric acid / phosphorylated pullulan / MTA |
|---|
| 研究開始時の研究の概要 |
CPN reduce the inflammation and induce numerous blood vessels in the dental pulp exposure area. PPA plays critical roles in bone regeneration and PPL used as a carrier for growth factors in hard tissue engineering. Based on the above-mentioned properties of CPN and carriers PPA/PPL, these materials combined with MTA/CH materials may contribute to the development of a novel experimental pulp capping material.
|
| 研究実績の概要 |
We aim to develop an experimental pulp capping material by the incorporation Colloidal platinum nanoparticles (CPN) with the commercially available pulp capping agent calcium hydroxide (CH). Initially, we assessed the pulpal tissue response in Wistar rats following the application of three different CPN materials at day 1, 7, 14, and 28 subsequent to direct pulp capping. The dental pulps were mechanically exposed and randomly divided into three groups based on the CPN materials applied: group 1- PAA-PT (platinum, poly acrylic acid, water), group 2- C-PT (platinum, sodium citric acid, water) and group 3- C-CYD (platinum, sodium citric acid, γ-cyclodextrin, water). All cavities were then restored using Super Bond-SB. A negative control (SB) was also included. At day 1, no or mild inflammatory responses were observed in all three CPN groups, significantly differing from the SB group (p<0.05), except C-PT group. At day 3, numerous blood vessels with no or mild inflammation were observed in the C- CYD group, indicating rapid angiogenesis, which was significantly different from SB groups (p<0.05), whereas PAA-PT and C-PT groups showed mild to moderate inflammatory responses. At day 7, no or mild inflammation was observed in the C-CYD group significantly differing from the PAA-PT, C-PT and SB groups (p<0.05), whereas mild or moderate inflammatory responses were observed in all other groups.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Last year, we evaluated the healing responses of the colloidal platinum nanoparticles (CPN).
CPN is being incorporated into pulp capping agent calcium hydroxide (CH). A positive control (CH) is also included.
The expression of angiogenesis, inflammation, and mineralization-related markers such as CD34, CD45, VEGFs; IL-6, IL-10; ALP, Runx2, OCN (immunohistochemistry) will be checked.
The mechanical properties including microhardness, compressive strength, setting time will be checked for screening out the optimal experimental pulp capping material.
On the other hand, the adhesion ability of the experimental pulp capping material to dental hard tissue is being evaluated (SEM, TEM, EDS, and FTIR).
|
| 今後の研究の推進方策 |
Histological preparation: by the end of October 2024, the histological response of exposed dental pulp to the combined material will be evaluated using Hematoxylin-Eosin staining (histological observation), details were taken concerning the hard tissue bridge (continuity, morphological aspects, thickness, localization); Mechanical properties analysis: by the end of December 2024; Immunohistochemical preparation: by the end of August 2025; Data acquisition: by the end of September 2025; Data analysis: by the end of October 2025; Preparation of a draft for the manuscript: by the end of January 2026; Manuscript submission for publication: by the end of March 2026.
In our present study evaluation, the healing responses of colloidal platinum nanoparticles on dental pulp were very satisfactory. Therefore, calcium hydroxide combined with colloidal platinum nanoparticles could be the material of choice for direct pulp capping, which will be our next future research work.
|
