| 研究課題/領域番号 |
23K16303
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分58020:衛生学および公衆衛生学分野関連:実験系を含む
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| 研究機関 | 広島大学 |
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研究代表者 |
KO KO 広島大学, 医系科学研究科(医), 助教 (80910299)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2025年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2024年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2023年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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| キーワード | Hepatitis D Virus / Cambodia / ELISA / Screening / Hepatitis D Virus |
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| 研究開始時の研究の概要 |
We will examine anti-HDV through opportunistic test by means of commercially available ELISA kit, and in-house developed direct ELISA and Sandwich method to all HBsAg positive samples from pregnant women and their newborns in Siem Reap, Cambodia followed by molecular analysis.
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| 研究実績の概要 |
I developed and validated an in-house direct ELISA method for detecting anti-HDV among 67 HBsAg positive pregnant women's stock serum collected in 2021 in Cambodia. Compared to a commercially available kit, the sensitivity was 50%, and the specificity was 95.4%. The overall anti-HDV prevalence among HBsAg positives was 5.97% (4/67). Subsequently, positive samples were tested for HDV RNA using nested RT-PCR with in-house developed universal primer sets, revealing that only 2 were HDV RNA positive (2.98%). Further amplification confirmed both belonged to HDV genotype 1. The study results were presented at APASL 2023 and the Japan Society of Hepatology conference in Otani. Currently, I am conducting a trial experiment on anti-HDV detection using dried blood spot (DBS) samples.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
Over fifty percent of the project has been completed after repeated trial and experiments on development of in-house Direct ELISA method. Subsequently, the trial experiment on anti-HDV detection by in-house direct ELISA method using dried blood spot samples is on track and I expect to develop and validate before September 2024. The, I will subsequently continue for anti-HDV detection among all 67 positives pregnant women DBS and their children DBS. The result of the study have been presented as poster in both Japan Society of Hepatology conference in Otani and APASL Kyoto 2024.
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| 今後の研究の推進方策 |
Subsequently after successful development and validation of In-house Direct ELISA based anti-HDV detection, I am now on track in developing in-house Direct ELISA based detection of anti-HDV using Dried Blood Spot samples, and planning to perform anti-HDV screening among children. The technique development is expected to complete by September 2024. Once the in-house method is validated, the anti-HDV screening will be performed in children using DBS samples.
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