| 研究課題/領域番号 |
23K19230
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0403:人間医工学およびその関連分野
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| 研究機関 | 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター) |
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研究代表者 |
Mohamed Aziz・Awaad・Aziz 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター), ナノ医療イノベーションセンター, 研究員 (50978286)
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| 研究期間 (年度) |
2023-08-31 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | polycarboxybetaine / immunogenicity / anti-tumor / drug delivery systems / pH-responsive |
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| 研究開始時の研究の概要 |
For effective tumor targeting and smart drug delivery, the pH-responsive PGLu(DET-Car)-based polymeric micelles will be used for tumor therapy. The obtained data from this protocol will introduce detailed information about smart nanocarriers used for anti-tumor drug delivery systems development.
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| 研究実績の概要 |
Successfully were prepared a polyzwitterion termed as PGLu(DET-car) was prepared with a pH-responsive against tumorous pH and with lower immunogenicity compared with conventional polymers. The PGLu(DET-car) polymer showed lower induction of serum immunoglobulin (IgM, IgG) in vivo and cytokines (IL-2, IFN‐γ) induction in vitro compared with polyethylene glycol (PEG) polymer. Additionally, conjugation of PGLu(DET-car) into PEG polymer reduced the Anti-PEG IgM in the blood serum. The polymeric micelles prepared from the PGLu(DET-car) showed compatible affinity to reach deeper in the tumor site compared with those prepared from PEG. Furthermore, the PGLu(DET-car) micelles showed lower induction of IgM in the blood serum, as well as lower induction of IL-2, IFN‐γ cytokines in vitro.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
We successfully prepared a pH-responsive PGLu(DET-car)-based micelle. Additionally, we evaluated the immunogenicity of the prepared micelle both in vitro and in vivo. The current data showed lower immunogenicity of PGLu(DET-car) polymer as well as PGLu(DET-car)-based micelle compared with conventional polymers.
Unfortunately, we found difficulty loading a drug in the prepared micelles. The drug-loaded micelle preparation still needs more characterization and evaluation. we found unstable micelle after loading with anti-cancer drugs. therefore, it will take some time to optimize he conditions of loading drugs in the prepared micelles.
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| 今後の研究の推進方策 |
During the current year we will evaluate the in vivo biodistribution of PGLu(DET-car)-based micelle. Additionally, anti-cancer or anti-aging drugs loading into the prepared micelle will be optimized and evaluated using different animal models.
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