| 研究課題/領域番号 |
23K19731
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0907:口腔科学およびその関連分野
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| 研究機関 | 松本歯科大学 |
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研究代表者 |
何 治鋒 松本歯科大学, 総合歯科医学研究所, 助教 (10980047)
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| 研究期間 (年度) |
2023-08-31 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | macrophage / bone repair / Wnt signal / Macrophage / Bone repair |
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| 研究開始時の研究の概要 |
Wnt signaling has been reported to be involved in bone mass regulation. The applicant's laboratory has revealed that the Wnt signaling network is involved bone metabolism. This time I came up with an idea about connects the skeletal stem cells, Wnt signaling network and macrophages (Mφ) (which have been considered as the dominant immune cell taking part in bone healing) together to establish the new strategy for investigating the complex and fascinating molecule interactions in the bone regeneration process.
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| 研究実績の概要 |
We identified the surface macrophage marker "A". Utilizing flow cytometry and staining with the A antibody, we were able to segregate F4/80+ Csf1r- cells into 3 distinct subpopulations: A high, A intermediate, and A low. Through comparison of cell frequencies and their responsiveness to clodronate liposome, we determined that A high F4/80+ Csf1r- cells are essential in bone repair.
We also identified the secreted protein factor X. In our RNA-seq data, where we compared F4/80+ Csf1r+ and F4/80+ Csf1r- cells, factor X emerged as one of the top 20 genes. Previous studies have associated its expression with granulocytes and macrophages. We observed its upregulation following bone injury and subsequent downregulation upon macrophage depletion, which was concurrent with changes in Wnt signaling.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Most of the objectives outlined in the grant application have been met successfully. Our research concepts are well-defined, and the experimental methodologies employed are strongly backed by resources available at our research institute.
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| 今後の研究の推進方策 |
After identifying factor "X", we will proceed with in vitro experiments. Our strategy involves introducing recombinant "X" into the culture medium of bone marrow stromal cells, with and without specific osteogenic agents. We will assess the impact of "X" on osteoblast differentiation through assays such as Alp or alizarin red staining. Additionally, we will evaluate the mRNA expression and protein levels of Wnts.
Although we initially planned to generate "A"-Cre "X" flox/flox conditional knockout (cKO) mice and observe their bone regeneration phenotype, we intend to start with Vav1-Cre instead. Finally, we will assess the effect of recombinant "X", with macrophage ablation through injection or Matrigel embedding at the bone injury site, to investigate its role in accelerating bone healing.
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