| 研究課題/領域番号 |
23K19925
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0909:スポーツ科学、体育、健康科学およびその関連分野
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| 研究機関 | 徳島大学 |
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研究代表者 |
ULLA ANAYT 徳島大学, 大学院医歯薬学研究部(医学域), 助教 (20980171)
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| 研究期間 (年度) |
2023-08-31 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | 3D-Clinorotation, / MAM, / Mitochondria, / Mulberry Polyphenols, / Microgravity, / Tail suspension, / ER Stress / mulberry polyphenol / MAM |
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| 研究開始時の研究の概要 |
Inactivity disrupts mitochondrial-associated ER membrane (MAM) integrity. So here I will investigate the effects of mulberry polyphenols in preventing MAM disruption thereby reducing metabolic abnormalities in inactivity-induced muscle using cell and animal model.
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| 研究実績の概要 |
Inactivity causes degradation of mitofusion-2 (MFN2) protein which disrupts mitochondrial-associated ER membrane (MAM) integrity. The MFN2 degradation further causes mitochondrial dysfunction and ER stress leading to metabolic abnormalities.
This year, I found by proximity ligation assay (PLA) analysis that 3D-clinorotation (microgravity-inducer) of C2C12 cells causes disruption of MAM structure. Furthermore, it decreases the MAM number, ATP production and MAM related protein, and increased the expression of Atrogin-1 and MuRF-1, markers for muscle atrophy. Morin and 4-oxyresveratrol were used against MAM disruption and related changes in C2C12 cells. This research is continued in siRNA treated MFN2 knokdown C2C12 cells. In the follow up study, animal experiment will be conducted.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The research that has been planned earlier is progressing smoothly with the time span. The step by step experiments and analysis are being conducted and analysed. I belive that within the stipulated time, research outcomes can be obtained from both the cell and animal studies.
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| 今後の研究の推進方策 |
Currently,I am investigating and analysing the 3D-clinorotated (Control and MFN2 knockdown) C2C12 cells treated with polyphenols (Morin and 4-oxyresveratrol) to check the MAM integrity, mitochondrial function, oxidative stress and metabolic changes. In the follow up study, the animal (In vivo) experiments will be conducted to illustrate the changes in MAM integrity and energy regulations with the treatments of polyphenols in tail suspension-induced control and MFN2 knockout mice. I will also analyze the gastrocnemius muscle glucose metabolism in MFN2 muscle-specific knockout mice treated with polyphenols.
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