| 研究課題/領域番号 |
23K20915
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| 補助金の研究課題番号 |
21H01253 (2021-2023)
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 基金 (2024)
補助金 (2021-2023) |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分19010:流体工学関連
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| 研究機関 | 熊本大学 |
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研究代表者 |
ホサノ ハミド 熊本大学, 産業ナノマテリアル研究所, 教授 (00543406)
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| 研究分担者 |
秀 拓一郎 北里大学, 医学部, 准教授 (40421820)
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| 研究期間 (年度) |
2021-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
16,510千円 (直接経費: 12,700千円、間接経費: 3,810千円)
2024年度: 3,770千円 (直接経費: 2,900千円、間接経費: 870千円)
2023年度: 3,900千円 (直接経費: 3,000千円、間接経費: 900千円)
2022年度: 4,030千円 (直接経費: 3,100千円、間接経費: 930千円)
2021年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円)
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| キーワード | ワクチン/ドラッグデリバリー / マイクロフルイディクス / バイオエンジニアリング |
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| 研究開始時の研究の概要 |
We will design, examine, and elucidate mechanisms/effectiveness of needleless, pain-free, inexpensive, small, controllable microjet vaccine/drug delivery systems. The main advantages are that they can be accurately controlled to deliver required amount of vaccine/drug to exact depth of dermis.
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| 研究実績の概要 |
The goal of the research for fiscal year 2023 (R 5) was defined as experimental evaluation and analysis of novel nanosecond pulsed discharge induced cavitation/shock wave micro-jets; quantitative in-vitro, ex-vivo, and in-vivo assessment; and publication.
According to the plan, new innovative models have been designed to minimize possibility of electrodes damage after pulse discharges, which could considerably improve durability of the microjet device. Real-time ultra-high-speed flow visualizations of micro-jets induced by different electrode configuration and energy models to control microjets penetration depth have been performed and the results were compared. Analysis of micro-jet shear stress induced poration in cell lines have been performed. Experimental and theoretical evaluation of how underwater shock waves and microstreaming can cause cell poration for effective vaccine/drug uptake have been carried. The results are discussed in a well-prepared manuscript. In order to refer to concern regarding possibility of any damage due to shock waves produced by the microjet injectors, we applied weak shock waves to glioblastoma cells and performed comprehensive gene expression analysis by using RNA-sequencing. The interesting results confirm the device is safe. We will continue the experiments to find the shock waves threshold to cause gene damage. Evaluation and optimization of the device based on the cells and ex-vivo results were performed. Preparation steps for in-vivo study and assessment of controlled release of vaccine and drug in mouse model have been performed.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The research has been progressing according to the plan. The goals for the year 2023 (R 5) have been achieved.
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| 今後の研究の推進方策 |
Based on progress achieved during year 2023 (R 5), measures have been taken for the research to advance as planned. Further publication, international conference presentation, and international collaborations have been planned to advance the research.
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