| 研究課題/領域番号 |
23K23941
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| 補助金の研究課題番号 |
22H02678 (2022-2023)
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 基金 (2024)
補助金 (2022-2023) |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分45020:進化生物学関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
LAUDET Vincent 沖縄科学技術大学院大学, 海洋生態進化発生生物学ユニット, 教授 (20898423)
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| 研究分担者 |
木下 政人 京都大学, 農学研究科, 准教授 (60263125)
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| 研究期間 (年度) |
2022-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
17,290千円 (直接経費: 13,300千円、間接経費: 3,990千円)
2025年度: 2,990千円 (直接経費: 2,300千円、間接経費: 690千円)
2024年度: 2,730千円 (直接経費: 2,100千円、間接経費: 630千円)
2023年度: 6,890千円 (直接経費: 5,300千円、間接経費: 1,590千円)
2022年度: 4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
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| キーワード | Pigmentation / Clownfish / Thyroid hormones / Ontogenesis / Thyroid Hormone |
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| 研究開始時の研究の概要 |
We hypothesize that differences in pigmentation pattern ontogeny between Amphiprion ocellaris and A. percula are linked to variations in thyroid hormone (TH) signaling. We will document and compare pigmentation patterns, measure TH levels, and analyze gene expression related to TH signaling. This study aims to uncover the developmental and genetic mechanisms behind phenotypic diversity in these species, offering insights into their ecological and evolutionary significance.
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| 研究実績の概要 |
How differences in pigmentation arose have never been fully explained. In anemonefishes there is a difference in white bar formation between Amphiprion ocellaris and A. percula. As white bars are linked to thyroid hormones (TH) we study if this ontogeny difference is linked to differences in TH signaling. We collected samples of the 2 species from hatching until 100 days. We have added the closely related A. biaculeatus to determine the ancestral status of bar formation. The samples are ready and will soon be sent for Illumina sequencing. We treated A. ocellaris with thyroid hormone and to MPI (to block TH production) and we submitted the samples to transcriptomic analysis. We have established the CRISPR/Cas9 technology in A. ocellaris and have targeted a first gene (dio2).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
OIST anemonefish husbandry construction and start have been delayed due to Covid and minor technical issues. This is now mostly solved. We need the husbandry that is in the main campus as OIST marine station can only host native species and A. percula is coming from Australia. All the research about this species have been delayed as we had to establish collaboration with foreign labs to get samples of this species.
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| 今後の研究の推進方策 |
1.We plan to check if the timing of the metamorphosis is similar in the two species using a comparative transcriptomic experiment. We have collected the samples of the 3 species (A. percula, A. ocellaris and A. biaculeatus) at various stages from hatching until the formation of second white bar in A. percula (100dph). The samples are ready and will soon be sent for Illumina sequencing.
2.We are analyzing the transcriptomic data of A. ocellaris treated with thyroid hormone and with MPI to block TH production to reveal the transcriptional cascade controlled by TH in this species.
3.With CRISPR/Cas9 technology Pr. Kinoshita lab have targeted a first gene in A. ocellaris (deiodinase 2). The mutant fish are growing. We will check the germline transmission of the mutation and analyze the phenotype.
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