| 研究課題/領域番号 |
23K23941
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| 補助金の研究課題番号 |
22H02678 (2022-2023)
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 基金 (2024)
補助金 (2022-2023) |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分45020:進化生物学関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
LAUDET Vincent 沖縄科学技術大学院大学, 海洋生態進化発生生物学ユニット, 教授 (20898423)
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| 研究分担者 |
木下 政人 京都大学, 農学研究科, 准教授 (60263125)
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| 研究期間 (年度) |
2022-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
17,290千円 (直接経費: 13,300千円、間接経費: 3,990千円)
2025年度: 2,990千円 (直接経費: 2,300千円、間接経費: 690千円)
2024年度: 2,730千円 (直接経費: 2,100千円、間接経費: 630千円)
2023年度: 6,890千円 (直接経費: 5,300千円、間接経費: 1,590千円)
2022年度: 4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
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| キーワード | Pigmentation / Clownfish / Thyroid hormones / Ontogenesis / Metamorphosis / Thyroid Hormones / Cora Reef Fishes / Ontogeny / Thyroid Hormone |
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| 研究開始時の研究の概要 |
We hypothesize that differences in pigmentation pattern ontogeny between Amphiprion ocellaris and A. percula are linked to variations in thyroid hormone (TH) signaling. We will document and compare pigmentation patterns, measure TH levels, and analyze gene expression related to TH signaling. This study aims to uncover the developmental and genetic mechanisms behind phenotypic diversity in these species, offering insights into their ecological and evolutionary significance.
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| 研究実績の概要 |
Our purpose is to test the hypothesis that difference existing in pigmentation pattern ontogeny between two different clownfish species (Amphiprion ocellaris and A. percula) are linked to differences in thyroid hormone (TH) signaling. We have determined the ontogenetic transcriptome of these two species plus an outgroup and generated Crispr/Cas9 mutants of a gene controlling TH formation
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Because of the delay in setting-up our husbandry the main acheivements of our research has been delayed but we have now an excellent dataset of developmental transcriptomic to analyse and we have generated a Crispr/Cas9 line that show germline transmission. We are therefore very confident that important results will be generated very soon.
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| 今後の研究の推進方策 |
The work is now going into two main direction
1° We have transcriptomic data of A. ocellaris, A. percula and Premnas biaculeatus from hatching until the end of trunk bar formation (more than 100 days) post hatching. We have published the first part of this analysis for A. ocellaris in Roux et al., Cell Reports 2023. We are currently working on the comparative transcriptomic analysis of the three species.
2° In collaboration with our co-I Masato Kinoshita we have developped a Crispr/Cas9 line in A. ocellaris with a mutated version of the deiodinase 2 gene. These fishes should have less TH and exhibit a phenotype similar to A. percula. We have now founder fish that grow and will be crossed to generate homozygotes.
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