| 研究課題/領域番号 |
23K25843
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| 補助金の研究課題番号 |
23H01146 (2023)
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 基金 (2024)
補助金 (2023) |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分13040:生物物理、化学物理およびソフトマターの物理関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
PIGOLOTTI Simone 沖縄科学技術大学院大学, 生物複雑性ユニット, 教授 (30812193)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
12,870千円 (直接経費: 9,900千円、間接経費: 2,970千円)
2025年度: 4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2024年度: 3,770千円 (直接経費: 2,900千円、間接経費: 870千円)
2023年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円)
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| キーワード | DNA replication / replisome / bacterial cell cycle |
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| 研究開始時の研究の概要 |
The theory we developed, combined with experiments, permits to shed light on the DNA replication program of a broad range of organisms. We will apply our method to a set of model microbial organisms in different conditions, to understand the strategies they implement to replicate their genomes.
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| 研究実績の概要 |
During this FY, we achieved two main goals of our project. The first goal has been to develop a general theory to describe the DNA abundance distribution from the genome replication dynamics. This general theory constitutes the basis of our approach. The paper based on this theory has been published in Plos Computational Biology. The second goal has been to startup our wet lab activity and successfully run the first benchmark experiments on E.coli. This success puts us in a very good position for completing the rest of the project.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The research is progressing as planned, with one paper already published and the groundwork for future experimental activity already done. We expect to soon make progress on applying our theory and experiment to novel systems, in particular to E.coli mutants and other microbial species, as detailed in the proposal of this project.
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| 今後の研究の推進方策 |
During this fiscal year, we expect to finalize our project on studying DNA replication in engineered E.coli strains with multiple origins of replication. Motivated by our recent theoretical results, we will also apply our method to budding yeast, to prove that our approach is not limited to prokaryotic species. We expect to obtain at least preliminary results on this second goal during this fiscal year.
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