| 研究課題/領域番号 |
23K26919
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| 補助金の研究課題番号 |
23H02226 (2023)
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 基金 (2024)
補助金 (2023) |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分39050:昆虫科学関連
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| 研究機関 | 順天堂大学 |
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研究代表者 |
グセフ オレグ 順天堂大学, 大学院医学研究科, 特任先任准教授 (30711999)
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| 研究分担者 |
黄川田 隆洋 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, グループ長 (60414900)
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| 研究期間 (年度) |
2023-04-01 – 2027-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
18,590千円 (直接経費: 14,300千円、間接経費: 4,290千円)
2026年度: 4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2025年度: 4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2024年度: 5,200千円 (直接経費: 4,000千円、間接経費: 1,200千円)
2023年度: 4,810千円 (直接経費: 3,700千円、間接経費: 1,110千円)
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| キーワード | anhydrobiosis / snRNAseq / brain / insect / cell populations / brain cell populations / neurons / single cell RNAseq / neuronal cells / cytoprotective molecules |
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| 研究開始時の研究の概要 |
This study aims to elucidate molecular and genetic mechanisms protecting against complete desiccation variety of cells in the brain of the sleeping chironomid and to identify compounds with neuroprotective effects for biomedical applications.
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| 研究実績の概要 |
This study aims to elucidate molecular and genetic mechanisms protecting against complete desiccation cells in the brain of the sleeping chironomid and to identify compounds with neuroprotective effects for biomedical applications.
To reveal the complexity of cell types and their destiny during anhydrobiosis, we conducted detailed single-nuclei transcriptomics profiling of the chironomid brains in six time points via the cycle of desiccation-rehydration. During this year we have optimized the larvae dissection for brain extraction and adaptation of cell dissociation protocol. We made an initial reconstruction of cell population composition dynamics and adaptation using single-cell mRNA expression events in the brain of the chironomid through the cycle of desiccation and rehydration.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
Based on the research plan we have successfully adapted the protocol of dissection of chironomid brain, followed by single-nuclei RNAseq analysis of six timed points of anhydrobiosis. As a result, we successfully profiled a total of 86 000+ nuclei. Due to rapid progress with T-2-T genome analysis of P. vanderplanki we achieved a total number of more than 600 genes per cell profiling. We identified key cell types by comparing P.v. and Drosophila data. Already on the current step of the study, we identified shifts in cell populations in response to desiccation and made the hypothesis about the specific vulnerability of some organelles to desiccation. We finally could indirectly confirm our predictions using analysis of Pv11 cell morphology. These results were published as a research paper.
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| 今後の研究の推進方策 |
As planned, we will the next major step: analysis of metabolomic events associated with anhydrobiosis in the brain of the larvae, using both metaboles profiling and in silico combination of transcriptomic and proteomic data from the brain of P. vanderplaki. We expect to obtain a profile of changes of up to 400 metabolites in the brains of the larvae. We will further list prospective candidates for metabolites, proteins, and peptides with predicted neuroprotective effects on human neurons by combining our gene expression and metabolomic data, literature survey, and molecular effect simulation. At the end of the year, we expect to obtain a list of promising candidates for further in vitro (and in vivo in the future) experiments for validation of protective potential for mammalian neurons.
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