| 研究実績の概要 |
In nature, bacteria and fungi coexist in the roots of plants while influencing the plants' growth and health. We isolated bacterial strains, specifically Pseudomonas strains, namely Psv and Pso, that can be detrimental to plants by producing unidentified volatile compounds.If the bacteria are grown in rich media such as LB media, the nearby plants will be wilted despite any no physical interaction, suggesting the production of harmful volatiles. However, these isolates can produce beneficial volatiles for plants when grown on low-nutrient media such as 3-times-diluted LB media. We have performed the whole genome sequencing for both strains and found that they share an operon, i.e., pyrroloquinoline quinone synthase (PQQ). This operon is related to the production of secondary metabolites that previous studies have shown to be related to the plant-growth promotion trait from the genus Pseudomonas. We have knocked out this gene from the genome of Pso, and we are going to proceed with the transformation and the inoculation test on the plants.
We have also performed the RNA-sequencing and analysis for Arabidopsis thaliana in the presence of Pso volatiles and found that the genes related to nitrate transporters were upregulated even though the plant media contains a high nitrate concentration. Furthermore, Pso's volatile on beneficial mode has been shown to protect the plants from the devastation caused by a pathogenic Colletotrichum fungus. These results indicate the role of bacterial volatiles in promoting plant growth and protecting plant health.
|
