| 研究課題/領域番号 |
23KF0090
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分46010:神経科学一般関連
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
風間 北斗 国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (90546574)
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| 研究分担者 |
CHERNG BOR-WEI 国立研究開発法人理化学研究所, 脳神経科学研究センター, 外国人特別研究員
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| 研究期間 (年度) |
2023-04-25 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,000千円 (直接経費: 2,000千円)
2024年度: 1,000千円 (直接経費: 1,000千円)
2023年度: 1,000千円 (直接経費: 1,000千円)
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| キーワード | olfactory processing / Drosophila / innate behavior / learned behavior |
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| 研究開始時の研究の概要 |
In this study aims to examine how innate and experience-dependent processing interact in the brain using Drosophila. Individual odors are associated with intrinsic values and drive innate behaviors such as avoidance and approach, yet they can be paired with reward or punishment to gain updated values. We will combine behavioral analysis in a virtual environment, two-photon calcium imaging, and quantitative data analysis to reveal the computation and mechanisms underlying the interaction between innate and learned centers to understand the basis of adaptive behavior.
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| 研究実績の概要 |
We developed an olfactory conditioning paradigm, which can be conducted under a two-photon microscope for simultaneous tracking of neural activity and behavior throughout learning. To monitor neural activity in behaving animals over the entire stages of learning, we devised a closed-loop system responding to the real-time state of a behaving fly, which is compatible with neural recording from multiple brain regions at single-cell resolution with two-photon calcium imaging. To alter the innate value of odors through training, we employed an olfactory conditioning paradigm, associating the value of reinforcers such as reward (e.g., sweet taste) or punishment (e.g., bitter taste) with odors. We also used optogenetic stimulation of gustatory receptor neurons as a reinforcer.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We were able to develop an olfactory conditioning paradigm compatible with simultaneous tracking of behavior and neural activity as planned. A setup to monitor the behavior and to deliver reinforcers (both real and optogenetically applied) is also successfully built.
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| 今後の研究の推進方策 |
In the second year, we aim to test the hypothesis that the conditioning changes olfactory representations in the LH, or in the upstream/downstream regions. First, given the presence of an anatomical input from the MB to the LH, we will test if olfactory representations in the LH are updated. Second, we will test if changes in olfactory representations take place in regions downstream of both the MB and the LH. Finally, we will test if learning can also modulate the earlier olfactory center. We will further examine the circuit mechanism underlying neural plasticity following conditioning. We will test whether learning-dependent changes in olfactory representations in the LH and downstream regions are induced by mechanisms intrinsic to each region or through interactions between the MB.
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