| 研究課題/領域番号 |
23KF0152
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分49050:細菌学関連
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| 研究機関 | 大阪大学 |
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研究代表者 |
飯田 哲也 大阪大学, 微生物病研究所, 教授 (90221746)
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| 研究分担者 |
HASSAN JAYEDUL 大阪大学, 微生物病研究所, 外国人特別研究員
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| 研究期間 (年度) |
2023-09-27 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,100千円 (直接経費: 2,100千円)
2025年度: 800千円 (直接経費: 800千円)
2024年度: 800千円 (直接経費: 800千円)
2023年度: 500千円 (直接経費: 500千円)
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| キーワード | Providencia / T3SS / whole genome sequencing / mutant |
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| 研究開始時の研究の概要 |
Type three secretion systems (T3SSs) are sophisticated nanomachinery used by a number of pathogenic microorganisms to exert their pathogenic effect. A genetically related T3SS was revealed on a plasmid carried by P. rustigianii. In this study, we will investigate the presence of T3SS genes in genus Providencia along with their location on the genome. Also, characterization of putative T3SSs effector proteins in Providencia spp. will be performed.
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| 研究実績の概要 |
The objective of the research was to examine the existence and locations of T3SS genes in Providencia, as well as to functionally characterize the injectosome. Initially, we screened Providencia isolates in our collection using PCR, followed by whole genome sequencing (WGS) to pinpoint the locations of T3SS genes. Through WGS, we identified the presence and precise locations of T3SS-related genes. Next, we are preparing mutants and complementary strains using various strategies for the functional analysis of the T3SS. Although no definitive conclusions can be drawn at this stage, we anticipate reporting interesting findings by the conclusion of this study.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The present study aimed to ascertain the presence of T3SS genes in Providencia. As intended, we examined several Providencia isolates and verified the presence of T3SS genes in some of them. To proceed with our next steps, we are currently preparing mutants and complementary strains to elucidate the functional traits of the T3SSs. At this juncture, we are optimistic about uncovering the role of T3SS in these Providencia strains and arriving at our final conclusions as scheduled. Hence, we anticipate that our work is advancing smoothly.
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| 今後の研究の推進方策 |
We're currently proceeding to step-2 of our research, involving a series of in vitro and in vivo experiments. We've selected specific genes and are now in the process of creating deletion mutants and constructing complementary strains. Our upcoming plan entails assessing the invasiveness and diarrheagenicity of the prepared strains through both in vitro and in vivo experiments. Once we've uncovered these traits, our goal shifts to understanding the role of T3SS genes in the interaction between Providencia strains (wild type, mutants, and complementary strains) and their host. To achieve this, we'll utilize live cell imaging and fluorescent microscopy techniques.
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