| 研究課題/領域番号 |
23KF0253
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分27040:バイオ機能応用およびバイオプロセス工学関連
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| 研究機関 | 大阪大学 |
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研究代表者 |
永井 健治 大阪大学, 産業科学研究所, 教授 (20311350)
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| 研究分担者 |
BOONS RANI 大阪大学, 産業科学研究所, 外国人特別研究員
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| 研究期間 (年度) |
2023-11-15 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
1,900千円 (直接経費: 1,900千円)
2025年度: 300千円 (直接経費: 300千円)
2024年度: 500千円 (直接経費: 500千円)
2023年度: 1,100千円 (直接経費: 1,100千円)
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| キーワード | Bioluminescent plant / Callus cultivation / 3D bio-printing / 3D plant cell culture |
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| 研究開始時の研究の概要 |
We envision to expand the DNA library available to produce gene constructs that i) can be triggered by specific physical or chemical inputs and that ii) produce a range of bioluminescent proteins that generate light at different wavelengths in plant cells.
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| 研究実績の概要 |
During this first stage, I acquired knowledge of genetically modifying plant cells. This led to the continuation of a collaborative research project based on fungal luciferase, and resulted in one new bioluminescent plant strain.
Regarding the 3D printing of plant cells, I chose widely available biopolymers, such as agarose and alginate as the main ink constituents. I produced some ink compositions based on microgels, which are not yet fully optimal and need further optimisation.
I also attempted first 3D printing tests with the bio-inks, containing plant callus cells. The latter has the tendency to form cell aggregates causing the nozzle to clog. This indicates the importance of optimising callus cultivation and collection for 3D printable bio-inks.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As I have foreseen, the first months were used for my adaptation and learning gene editing techniques as well as gathering the necessary equipment or connecting with the right collaborators to use theirs, and adapting the protocols to the available equipment.
I have defined the platform requirements and have been conducting preliminary experiments to tackle the complications associated with 3D printing of plant cells. The results achieved so far have helped me gain a better understanding of the system and possible pitfalls, allowing me to redirect the next steps towards achieving the set goals.
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| 今後の研究の推進方策 |
Firstly, in the scope of the bioluminescent variants, my aim is to prepare a liquid callus culture from the newly obtained bioluminescent plant. Further, in collaboration, I aim to establish other colour variants to expand the available palette of bioluminescent plant callus.
Next, my objective is to establish a robust protocol for obtaining callus cells in sizes that allow use in high density plant cell 3D printing.
Finally, based on the results for the microgel-based agarose gels, I will conduct further tests towards the best composition for these 3D printing inks, including mechanical characterisation. Afterwards, I will consider the 3D design and post-printing conditions for optimal survival and bioluminescence emission.
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