| 研究課題/領域番号 |
23KF0286
|
|---|
| 研究種目 |
特別研究員奨励費
|
| 配分区分 | 基金 |
|---|
| 応募区分 | 外国 |
|---|
| 審査区分 |
小区分46030:神経機能学関連
|
|---|
| 研究機関 | 沖縄科学技術大学院大学 |
|---|
研究代表者 |
銅谷 賢治 沖縄科学技術大学院大学, 神経計算ユニット, 教授 (80188846)
|
|---|
| 研究分担者 |
GAMANUT ANDREI-RAZVAN 沖縄科学技術大学院大学, 神経計算ユニット, 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2023-11-15 – 2026-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
2,000千円 (直接経費: 2,000千円)
2025年度: 700千円 (直接経費: 700千円)
2024年度: 1,100千円 (直接経費: 1,100千円)
2023年度: 200千円 (直接経費: 200千円)
|
|---|
| キーワード | Claustrum / Retrosplenial cortex / Default Mode Network / Serotonin / Calcium imaging / Opto-genetics / Spiking neural networks |
|---|
| 研究開始時の研究の概要 |
The project will be subdivided in two parts, each with a specific aim: Aim 1 (experimental): reveal the response of claustrum neurons to serotonin, and the accompanying changes in behaviour; Aim 2 (computational): model how different types of serotonin receptors fine-tune the claustrum, and the effect of the tuning on the cortical dynamics.
|
| 研究実績の概要 |
Experiments. Injections of retrograde viruses were successfully made in retrosplenial cortex, and the result was infection of neurons in claustrum. These injections tested the quantity of virus that induces the optimal expression of fluorescent proteins in the claustrum, which will be used for imaging of calcium activity. Also, tests were made to precisely implant the prism-GRIN probes from Inscopix next to the claustrum, and the success was confirmed with micro-CT scans of the brain.
Computational model. The interaction between claustrum and a simplified cortical region was simulated. The model consists of ~ 12,000 point neurons. The adaptive exponential integrate and fire neuronal model fitted the dynamics of the claustrum neurons, while the cortical neurons were of Hodgkin-Huxley type.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
There were no major events to interfere with the initial plan of research. The experiments can be continued as planned, with no major revisions. It is expected that the control of serotonin release in the claustrum can be achieved following the current experimental design. The computational model contains sufficient details to explore the dynamics of the interaction between claustrum and cortex and the influence of serotonin can be introduced in the model as planned.
|
| 今後の研究の推進方策 |
Experimental. As originally planned, mice will be monitored during tasks, periods of awake rest and periods of sleep. Simultaneously, first the Calcium activity of the retrosplenial-projecting claustrum neurons will be imaged and their collective dynamics will be associated to each behavioural state. Then, the release of serotonin in the claustrum will be controlled with opto-genetics, and the impact on neuronal dynamics and on behaviour will be assessed.
Computational. First, simulations of the claustrum-cortex model will be done, each with a unique set of parameters, to explore the possible states of the system. Then, as planned, the effects of serotonin on the claustrum will be simulated by introducing equivalent currents of major serotonergic receptors in simulated claustrum neurons.
|
