| 研究課題/領域番号 |
23KJ2167
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分90120:生体材料学関連
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| 研究機関 | 国立研究開発法人物質・材料研究機構 |
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研究代表者 |
王 洪欣 国立研究開発法人物質・材料研究機構, 高分子・バイオ材料研究センター, 特別研究員(RPD)
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| 研究期間 (年度) |
2023-04-25 – 2026-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
3,900千円 (直接経費: 3,000千円、間接経費: 900千円)
2025年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2024年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2023年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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| キーワード | intracellular force / stress / AFM indentation |
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| 研究開始時の研究の概要 |
90% of cancer deaths is due to metastasis. It is important to evaluate metastatic potential at an earlier stage. I plan to develop a new method based on prestress measurement on cytoskeleton. Unlike conventional cancer type-dependent diagnosis, my method can be generally applied to all cancer types.
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| 研究実績の概要 |
We invented a unique way to‘touch’a cell with nanoscale‘hand’, so that the intracellular force (prestress) distribution, rather than commonly discussed modulus, over a complete cell could be mapped by atomic force microscopy (AFM). Such task was achieved by using an informatics-assisted AFM indentation technique to search for both the models used to fit indentation force-displacement curves and probe geometry descriptors. It enabled lateral prestress to be mapped with nanometric details.
Furthermore, we visualized dynamic mechanical responses of the cells adapting to environmental stimuli in situ. Our results highlight the alteration in the intracellular forces to attenuate environmental stimuli, such as rescue from mechanical stimulus-initiated cell death and initiation of cell migration.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
This result was not only published in STAM and selected as Editor's choice, but also received an excellent poster award at an academic conference.
In addition, AFM measurements were used in research to develop cell scaffolds based on ionic liquid interface, and the results were published in Adv. Mater.
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| 今後の研究の推進方策 |
Following the demonstrated examples (Wang, etc. STAM, 2023) and power of this new characterization technique, future development should be further improving force sensitivity toward single molecule force measurement. It would enable direct understanding of mechanical roles for each molecular component in the cell response circuitry, when combining the technique with state-of-the art molecular imaging technologies.
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