| 研究課題/領域番号 |
24K08447
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分34010:無機・錯体化学関連
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| 研究機関 | 九州大学 |
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研究代表者 |
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| 研究期間 (年度) |
2024-04-01 – 2027-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2026年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2025年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | metal-organic polyhedra / coordination cages / protein cross-linking / Enzyme / biocomposite / hybrid materials / 金属－有機多面体 (MOP) / 酵素複合体 / 酵素固定化 |
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| 研究開始時の研究の概要 |
本課題では、簡便かつ極めて温和な条件で運用可能な「水溶性金属－有機多面体（MOP）を用いた酵素固定化法」を様々な酵素に適用できる技術に成熟させて、酵素固定化法として一般化する。更に、配位子修飾によるMOP表面の設計、MOP-酵素間相互作用の解明およびMOP-酵素複合体の最適化により、異なるスケールの観点から研究を展開し、特定の酵素に対するMOPのカスタムメイド設計指針の確立を目指す。
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| 研究実績の概要 |
In FY 2024-2025, the research project has been developed along 3 axes:
1) The applications to mixed-protein systems of previously described method. This led to a much higher control of materials composition, and allows co-immobilization of several enzymes to achieve cascades. This results led to one article (ACS Apll. Mater), and one oral presentation at CSJ nenkai.
2) The development of MOP-based salts, to offer more versatility in the immobilization matrices properties, without relying on proteins to form a continuous network. This axes is well advanced, and has been presented at CSJ nenkai.
3) The development of new water-stable and noble-metal-free MOPs based on thiocarboxylate ligands. Significant progress have been done last year.
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| 現在までの達成度 |
現在までの達成度
1: 当初の計画以上に進展している
理由
For research axis (1), a first article has been published in ACS applied materials Interfaces (IF=8.5). The project is now in a implemenatation stage for technical systems. Good results have also been obtained with the co-immobilization of enzymes (-> cascade reactions), as well as the use of cascade knock-down for sensing, notably of cyanide ions.
- For axis (2), the MOP-salts systems have been established, as-well-as tyhe efficient immobilization of enzymes and performance retention. Now, the project is in the stage of data aquisition for publication.
- For axis (3), new structures have been obtained. Now, systematic modification of the MOP are ongoing, to obtained interesting functional properties and notabvly water-solubility (in addition to stability).
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| 今後の研究の推進方策 |
The three research aspect of the project are going well. With the collaboration of research students, the projects willl be developped further.
- Axis (1) will be driven towards application. In particular, interaction with biocatalysis groups for the implementation of industry relevant cascade reaction are considered. The application of the system to sensing of cyanide is also promising.
- Axis (2) systems demonstrate a high versatility of structure and composition. After a first report (planned this fical year), the system will be developed further to implement additional functional properties.
- Axis (3) systems showed promising properties. Furthermore, the systems showed also very good performance for ammonia capture. The system will be explored systematically before reporting.
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