| 研究課題/領域番号 |
24K15694
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分90110:生体医工学関連
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| 研究機関 | 山形大学 |
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研究代表者 |
カジィ グルサンアラシャティ 山形大学, 大学院理工学研究科, 助教 (40713962)
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| 研究分担者 |
右田 聖 山形大学, 大学院理工学研究科, 准教授 (00512302)
有馬 ボシールアハンマド 山形大学, 大学院理工学研究科, 准教授 (30596549)
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| 研究期間 (年度) |
2024-04-01 – 2027-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2026年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2025年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2024年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
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| キーワード | Drug delivery system / Zinc / 亜鉛 / 副交感神経支配 |
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| 研究開始時の研究の概要 |
副交感神経節（PSG）が損傷すると、顎下腺の正常な機能が低下し、口内乾燥症などの健康問題を引き起こす。亜鉛（Zn2+）は、正常な脳の発達と機能に不可欠である。我々は亜鉛が顎下腺の成長を促進することを明らかにした。しかしながら、顎下腺における副交感神経の活動について、亜鉛がどのように影響するかについては、ほとんど調べられてこなかった。亜鉛摂取に使用される薬物送達システムについてはいくつか報告があるが、亜鉛の局所放出制御に関する報告はなく、副交感神経に対するその効果と作用機序はまだ不明である。本研究は、神経再生と亜鉛との関係を明らかにするために、感光性ナノゲルによる亜鉛供給の時空間制御を目指す。
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| 研究実績の概要 |
In this study, we aim to develop a novel DDS for the targeted administration of zinc. A further objective is to elucidate the specific role of zinc in modulating parasympathetic innervation. During the first year of the project, we examined the effects of zinc on salivary gland development. Using an in vitro culture system, we found that zinc supplementation significantly enhanced glandular tissue growth and notably increased the elongation and distribution of neural cells. To validate these findings, we employed a mechanically stiffness-controlled culture environment designed to inhibit salivary gland tissue growth. Remarkably, the addition of zinc promoted tissue development. To explore the underlying mechanisms, we utilized various signaling pathway inhibitors. Our results indicate that zinc's influence on both salivary gland tissue growth and parasympathetic neural activity is mediated through the AKT signaling pathway. These findings provide novel insights into the critical role of zinc in the early developmental stages of salivary gland tissues and fulfilling one of the primary objectives of this study. Regarding the DDS, we have successfully synthesized the compound cyclo[RGD(DMNPB)fK], and future work will focus on evaluating its performance and further use in developmental processes with greater precision. In parallel, we worked on electrochemical sensor modifications, nano and microparticles synthesis. Using the obtained results, we have presented five papers at domestic and international conferences and published two academic papers.
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| 現在までの達成度 |
現在までの達成度
2: おおむね順調に進展している
理由
We have successfully presented significant research findings at the international conference, achieving one of the goals of this study: to elucidate the specific role and underlying mechanisms of zinc in the submandibular gland and the parasympathetic neural innervation. These results, derived from our details experiment, contribute valuable new insights to the field of life sciences. Overall, the research is proceeding smoothly and in line with the intended goals.
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| 今後の研究の推進方策 |
In the future, we plan to examine the results obtained so far, particularly the functional ability of cyclo [RGD(DMNPB)fK] on salivary gland tissue growth, using cyclo[RGD(DMNPB)fK] and the RGD sequence alone. We will continue to optimize the material use as a method of local DDS. We also plan to use a similar method to investigate the effect of zinc on neuron cells. We also plan to conduct more detailed quantitative evaluations. As for in vivo, we aim to construct a gel sheet to use for localized zinc supplement, and will further proceed with qualitative and quantitative evaluations
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