| 研究領域 | 脳構築における発生時計と場の連携 |
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| 研究課題/領域番号 |
19H04793
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| 研究種目 |
新学術領域研究(研究領域提案型)
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| 配分区分 | 補助金 |
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| 審査区分 |
生物系
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Moore Adrian 国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (30442932)
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| 研究期間 (年度) |
2019-04-01 – 2021-03-31
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| 研究課題ステータス |
完了 (2020年度)
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| 配分額 *注記 |
9,100千円 (直接経費: 7,000千円、間接経費: 2,100千円)
2020年度: 4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2019年度: 4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
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| キーワード | Neuron growth / chromatin regulators / transcription programs / Dendrite / Transcription Factor / Microtubule / Chromatin / Neuron Differentiaiton / neuron / dendrite / differentiation / temporal program / myosin / microtubules / actin / diffrentiation / time |
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| 研究開始時の研究の概要 |
To determine how developmental clock processes, regulate dendrite arbor patterning, recently we used in vivo live imaging of Drosophila c sensory neurons to follow the complete temporal sequence of arbor patterning events. Key to this is the timing of a switch in growth rates: early establishment of the main arbor field requires rapid outgrowth; this then transitions into a slow growing maturation phase. We predict that the developmental clock which times this transition consists of both a cell intrinsic counting component and anexternal regulatory component.
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| 研究実績の概要 |
The nervous system expands massively in size from its formation during embryogenesis until maturity; insufficient brain growth is a significant cause of intellectual disability. As neurons don’t divide, this expansion is supported primarily through enormous cellular growth that scales proportionally with the growth of the organism. Here, we utilized Drosophila larval body wall sensory neurons as a model system to investigate this scaling program. We carried out genome-wide mapping of transcription start sites at single base pair-resolution during scaling. Through motif- and Chip seq- activity mapping, we identified the prevalent chromatin architectural proteins that drive these promoter cohorts to power cellular scaling. In parallel, through systematic genetic screening of the loci controlled by these promoter cohorts, coupled with multi-dimensional morphometric analysis, we identified key cell biological effectors of this program. We found that these effectors of scaling also introduce variance to dendritic parameters of individual neurons, allowing for diversification of arbor morphology;a trait argued to potentially contribute to the emergence of higher order nervous system activity. Some neuron types are bigger than others; for example, the large class IV da neurons grow quicker and to a larger mature arbor size than other body wall sensory neurons.
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| 現在までの達成度 (段落) |
令和2年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和2年度が最終年度であるため、記入しない。
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