| 研究領域 | 高速分子動画法によるタンパク質非平衡状態構造解析と分子制御への応用 |
|---|
| 研究課題/領域番号 |
20H05438
|
|---|
| 研究種目 |
新学術領域研究(研究領域提案型)
|
| 配分区分 | 補助金 |
|---|
| 審査区分 |
複合領域
|
|---|
| 研究機関 | 東京工業大学 |
|---|
研究代表者 |
Maity Basudev 東京工業大学, 生命理工学院, 特任助教 (60815421)
|
|---|
| 研究期間 (年度) |
2020-04-01 – 2022-03-31
|
| 研究課題ステータス |
完了 (2021年度)
|
| 配分額 *注記 |
3,900千円 (直接経費: 3,000千円、間接経費: 900千円)
2021年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2020年度: 2,210千円 (直接経費: 1,700千円、間接経費: 510千円)
|
|---|
| キーワード | CO release reaction / Initial intermediate / TR-SFX method / Lysozyme / Serial crystallography / Lysozyme-Mn(CO)3 / CO photo-release |
|---|
| 研究開始時の研究の概要 |
Recently, using X-ray free electron laser (XFEL) , various dynamic processes associated with proteins have been elucidated. However, all such studies by serial femtosecond crystallography have been limited to only natural proteins and the methodology has not been applied tosynthetic organic molecules or metal complexes. In this work, we plan to establish a method to study process of a chemical reaction by serial crystallography. We will study the CO photo-release reaction from Lys-Mn(CO)3 composite and ring-closing-opening reaction of photochromic diarylethene molecules.
|
| 研究実績の概要 |
In this research, we demonstrated a visualization of photolysis reaction by a synthetic metal complex using TR-SFX method. We fixed a photoactive CO releasing Mn(CO)3 reaction center into porous Lysozyme microcrystals. X-ray crystal structure revealed an octahedral Mn reaction center with 3CO and two water molecules. Three CO ligands oriented in a facial manner. By controlling the time and light dose, we succeeded in determining the CO release process and structure of a bicarbonyl intermediate species in real-time. In addition, we performed quantum chemical calculations which was in good agreement with the experimental results. The demonstration has a great scope to explore the mechanism of difficult organic, inorganic, or synthetic metalloprotein reactions.
|
| 現在までの達成度 (段落) |
令和3年度が最終年度であるため、記入しない。
|
| 今後の研究の推進方策 |
令和3年度が最終年度であるため、記入しない。
|
