| 研究領域 | グリアデコーディング：脳-身体連関を規定するグリア情報の読み出しと理解 |
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| 研究課題/領域番号 |
23H04148
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| 研究種目 |
学術変革領域研究(A)
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| 配分区分 | 補助金 |
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| 審査区分 |
学術変革領域研究区分(Ⅲ)
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| 研究機関 | 筑波大学 |
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研究代表者 |
ラザルス ミハエル 筑波大学, 国際統合睡眠医科学研究機構, 教授 (80469650)
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| 研究期間 (年度) |
2023-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
13,000千円 (直接経費: 10,000千円、間接経費: 3,000千円)
2024年度: 6,500千円 (直接経費: 5,000千円、間接経費: 1,500千円)
2023年度: 6,500千円 (直接経費: 5,000千円、間接経費: 1,500千円)
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| キーワード | Gliosomnia / Immunity / Glia / sleep / glia |
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| 研究開始時の研究の概要 |
Immune cell differentiation and proliferation are known to be synchronized with sleep. However, the molecular and cellular mechanisms by which sleep regulates immune system function and the contribution of glia to the regulation of sleep (gliosomnia) are an enigma. Gene expression in nucleus accumbens glia and leukocytes from wild-type mice, sleep-deprived mice, and mutant mice with high sleep need will be analyzed using single-cell gene expression analysis and identify molecules that interfere with the regulation of the immune system.
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| 研究実績の概要 |
We successfully demonstrated sleep induction in the NAc of freely behaving mice by increasing the activity of extracellular adenosine derived from astrocytes and neurons with a photoactivatable allosteric modulator of adenosine A2A receptors. We also showed that cytokine production in microglia during wakefulness is important for maintaining wakefulness, demonstrating the importance of glia-mediated neuroimmune interactions in the sleep-wake cycle.
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| 現在までの達成度 |
現在までの達成度
2: おおむね順調に進展している
理由
The paper, "Optochemical control of slow-wave sleep in the nucleus accumbens of male mice by a photoactivatable allosteric modulator of adenosine A2A receptors," has been published in the high-impact journal Nature Communications.
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| 今後の研究の推進方策 |
We continue to perform biochemical and genetic experiments to analyze the function of inflammation-related genes in the NAc. These analyses may help to unravel microglia-neuron interactions in sleep/wake regulation, provide new insights into the link between sleep homeostasis and immune resilience, and identify potential therapeutic targets for the treatment of sleep disorders.
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