1989 年度実績報告書

酵素機能の化学的展開に関する研究

研究課題

研究課題/領域番号	01303007
研究機関	大阪大学
研究代表者	大城芳樹大阪大学, 工学部, 教授 (70028984)
研究分担者	木村栄一広島大学, 医学部, 教授 (30034010) 生越久靖京都大学, 工学部, 教授 (90026188) 大野淳吉京都大学, 化学研究所, 教授 (70027077) 井上祥平東京大学, 工学部, 教授 (20010762) 青山安宏長岡技術科学大学, 工学部, 教授 (00038093)
キーワード	酵素モデル / 補酵素モデル / 分子認識 / 細胞認識 / 金属錯体
研究概要	平成元年度までの研究経過を以下に示す。 1.酸化換言酵素のモデルとして主に補酵素PQQやフラビンモデルを取り上げ、それらの機能解明およびそれらを用いた高効率的触媒反応を見いだした(大城、新海、村橋)。2.Glycerol Dehydrogenaseの反応系において基質の反応性と立体化学の間の興味深い関係を見いだした(大野)。3.亜鉛酵素モデルとしてイミダゾ-ル基を有する機能性界面活性剤を合成し、エステル加水分解に対して大きな触媒効果を見いだした(太垣)。4.新規「かご型」シクロファンを合成し、その興味深い分子取り込み能を見いだした(村上)。5.リン酸基の関与する酵素反応に対するモデルとしてリン酸基の多点分子認識捕捉について検討し興味深い結果を得た(青山)。6.細胞認識性リポソ-ムとしてガラクトサミン末端を有するプルラン誘導体で被覆したリポソ-ムを合成した(砂本)。7.酵素および酵素モデルの合成二分子膜による選択的取り込みに関する興味深い結果を得た(国武)。8.新規なポルフィリン配位子の合成に成功した(井上、生越)。9.ルテニウムポルフィリン錯体を用いた高効率酸素触媒転移反応を見いだした(広部)。10.大環状ポリアミンの2量体を合成し、その選択的捕捉能を見いだした(木村)。11.金属酵素モデルのペプチド錯体の合成および構造と反応性について興味深い結果を得た(中村)。12.モリブデンおよびタングステンに配位した窒素分子の反応性を明らかにした(干鯛)。オキシヘモシアニンやチロシナ-ゼモデルとしてのμ-パ-オキソニ核銅錯体の合成に成功した(諸岡)。

研究成果
(26件)

すべてその他

すべて文献書誌 (26件)

[文献書誌] Yoshiki Ohshiro: "Preparation and Characterization of Iminoquinone and Aminophenol Derivatives of Coenayme PQQ" Tetrahedron Lett.30. 6875-6878 (1989)
[文献書誌] Yoshiki Ohshiro: "Importance of the Pyrrole Proton of Coezyme PQQ for the Efficient Amine-Oxidation" J.Am.Chem.Soc.,in contribution.
[文献書誌] Yasuhiro Aoyama: "Molecular Recognition of Nucleotides:Concurrent Hydrogen Bonding and Electrostatic Interactions in-the Two-point Fixation of Flavin Mononucleotide" Chem.Lett.in contribution.
[文献書誌] Yasuhiro Aoyama: "Multiple Hydrogen Bonding Fixation of Phosphates with Protonated Phyidylporphyrin;Discrimination of Monoalkyl and Dialkyl Phosphates" Angew.Chem.Int.ED.Engl.in contribution.
[文献書誌] Shohei Inoue: "Syntheses and Reactions of Aluminum Complexes of Capped Porphyrin" Tetrahedron,in press.
[文献書誌] Atsuyoshi Ohno: "Stereochemical Control in Microbial Reduction.12.(s)-4-Nitro-2-butanol as a Source to Synthesize natural Products." Bull.Chem.Soc.,Jpn,. 63. 91-96 (1990)
[文献書誌] Atsuyoshi Ohno: "A Novel Method to Synthesize(L)-β-Hydroxy Esters by the Reduction with Bakers' Yeast" Tetrahedron Lett.in press.
[文献書誌] Atsuyoshi Ohno: "NAD(P)^+-NAD(P)H Models.69.Mechanism of Stereospecific(Net)Hydride Transfer Controlled by Electronic Effect" Bull.Chem.Soc.,Jpn.in press.
[文献書誌] Toyoki Kunitake: "二分子膜" 有機合成化学協会誌. 47. 535-546 (1989)
[文献書誌] Toyoki Kunitake: "Biomimetic Functions of Sythetic Bilayer Menbranes" “Frontiers of Macromolecular Science",Proc.IUPAC 32nd Lnt.Symp.Kyoto,Japan.543-548 (1989)
[文献書誌] Toyoki Kunitake: "Direct Observation of Helices and Vesicles of Ammonium Bilayers by High-Resolution, Scanning Electron Microscopy" Chem.Lett.25-28 (1990)
[文献書誌] Seiji Shinkai: "Coenzym Models 47.Synthesis and Reactivity Studies of Novel Flavinophanes and 5-Deazaflavinophanes:Correlation between Flavin Reactivity and Ring Strain" J.Am.Chem.Soc.111. 4928-4935 (1989)
[文献書誌] Seiji Shinkai: "Coenzym Model 48.Novel Diastereo-Differentiating Hydrogen Transfer and“Rope-Skipping"Rasemization in Chiral Flavinophenes and 5-Deazaflavinophenes" J.Am.Chem.Soc.111. 4935-4940 (1989)
[文献書誌] Seiji Shinkai: "Specific Inhibition of Flavin Catalyses by a“Molecular Hinge"" Tetrahedron Lett.30. 5895-5898 (1989)
[文献書誌] Seiji Shinkai: "Coenzym Model 51.Structure and Reactivity Studies of 5-Methyl-5-deazaflavinophenes:On the“Axial Preference"in a Flavin Redox System" Bioorg.Chem.17. 344-358 (1989)
[文献書誌] Junzo Sunamoto: "Development of Cell-Specific Liposome and Its Application in Biotechnology" Nippon Kagaku-Kaishi. 161-173 (1989)
[文献書誌] Junzo Sunamoto: "Cell Targetability of Liposome Bearing Molecular Recognition" J.Jap.,Soc.D.D.S.4. 7-11 (1989)
[文献書誌] Junzo Sunamoto: "Cell Specificity of Polysaccharide Derivatives on Liposoma Surface" Chem.Lett.in press.
[文献書誌] Akira Nakamura: "Water Sensitivity of a (Fe_4S_4(SR)_4)^1-Cluster and Its Stabilization" Chem.Lett.399-402 (1989)
[文献書誌] Masanobu Hidai: "Catalytic Conversion of Molecular Nitrogen into Silylamines Using Molybdenum and Tungsten Dinitrogen Complexes" J.Am.Chem.Soc.111. 1939-1940 (1989)
[文献書誌] Masanobu Hidai: "Syntheses and Some Reactions of Trimethylsilylated Dinitrogen Complexes of Tungsten and Molybdenum" Bull.Chem.Soc.,Jpn.62. 2953-2959 (1989)
[文献書誌] Masaaki Hirobe: "Highly Efficient Epoxidation of Olefins With Phridine N-Oxides Catalyzed by Ruthenium Porphyrins" Tetrahendron Lett.30. 6545-6548 (1989)
[文献書誌] Yukito Murakami: "pH-Dependent Molecular Recognition Performed by a Polycationic Cubic Azaparacyclophone" Chem.Lett.1199-1202 (1989)
[文献書誌] Shunichi Murahashi: "Flavin-Catalyzed Oxidation of Amines and Sulfur Compounds with Hydrogen Peroxide" J.Am.Chem.Soc.111. 5002-5003 (1989)
[文献書誌] Yoshihiko Morooka: "μ-η^2:η^2=Peroxo Binuclesr Copper Complex,(Cu(HB93,5-i-Pr_2pz)_3)_2(O_2)" J.Am.Chem.Soc.111. 8975-8976 (1989)
[文献書誌] Yoshihiko Morooka: "Formation and Characterization of Mononuclear Acylperoxo Copper(II)Complex" Inorg.Chem.in press.

1989 年度 実績報告書

酵素機能の化学的展開に関する研究

研究代表者

大城 芳樹 大阪大学, 工学部, 教授 (70028984)

研究成果

[文献書誌] Yoshiki Ohshiro: "Preparation and Characterization of Iminoquinone and Aminophenol Derivatives of Coenayme PQQ" Tetrahedron Lett.30. 6875-6878 (1989)

[文献書誌] Yoshiki Ohshiro: "Importance of the Pyrrole Proton of Coezyme PQQ for the Efficient Amine-Oxidation" J.Am.Chem.Soc.,in contribution.

[文献書誌] Yasuhiro Aoyama: "Molecular Recognition of Nucleotides:Concurrent Hydrogen Bonding and Electrostatic Interactions in-the Two-point Fixation of Flavin Mononucleotide" Chem.Lett.in contribution.

[文献書誌] Yasuhiro Aoyama: "Multiple Hydrogen Bonding Fixation of Phosphates with Protonated Phyidylporphyrin;Discrimination of Monoalkyl and Dialkyl Phosphates" Angew.Chem.Int.ED.Engl.in contribution.

[文献書誌] Shohei Inoue: "Syntheses and Reactions of Aluminum Complexes of Capped Porphyrin" Tetrahedron,in press.

[文献書誌] Atsuyoshi Ohno: "Stereochemical Control in Microbial Reduction.12.(s)-4-Nitro-2-butanol as a Source to Synthesize natural Products." Bull.Chem.Soc.,Jpn,. 63. 91-96 (1990)

[文献書誌] Atsuyoshi Ohno: "A Novel Method to Synthesize(L)-β-Hydroxy Esters by the Reduction with Bakers' Yeast" Tetrahedron Lett.in press.

[文献書誌] Atsuyoshi Ohno: "NAD(P)^+-NAD(P)H Models.69.Mechanism of Stereospecific(Net)Hydride Transfer Controlled by Electronic Effect" Bull.Chem.Soc.,Jpn.in press.

[文献書誌] Toyoki Kunitake: "二分子膜" 有機合成化学協会誌. 47. 535-546 (1989)

[文献書誌] Toyoki Kunitake: "Biomimetic Functions of Sythetic Bilayer Menbranes" “Frontiers of Macromolecular Science",Proc.IUPAC 32nd Lnt.Symp.Kyoto,Japan.543-548 (1989)

[文献書誌] Toyoki Kunitake: "Direct Observation of Helices and Vesicles of Ammonium Bilayers by High-Resolution, Scanning Electron Microscopy" Chem.Lett.25-28 (1990)

[文献書誌] Seiji Shinkai: "Coenzym Models 47.Synthesis and Reactivity Studies of Novel Flavinophanes and 5-Deazaflavinophanes:Correlation between Flavin Reactivity and Ring Strain" J.Am.Chem.Soc.111. 4928-4935 (1989)

[文献書誌] Seiji Shinkai: "Coenzym Model 48.Novel Diastereo-Differentiating Hydrogen Transfer and“Rope-Skipping"Rasemization in Chiral Flavinophenes and 5-Deazaflavinophenes" J.Am.Chem.Soc.111. 4935-4940 (1989)

[文献書誌] Seiji Shinkai: "Specific Inhibition of Flavin Catalyses by a“Molecular Hinge"" Tetrahedron Lett.30. 5895-5898 (1989)

[文献書誌] Seiji Shinkai: "Coenzym Model 51.Structure and Reactivity Studies of 5-Methyl-5-deazaflavinophenes:On the“Axial Preference"in a Flavin Redox System" Bioorg.Chem.17. 344-358 (1989)

[文献書誌] Junzo Sunamoto: "Development of Cell-Specific Liposome and Its Application in Biotechnology" Nippon Kagaku-Kaishi. 161-173 (1989)

[文献書誌] Junzo Sunamoto: "Cell Targetability of Liposome Bearing Molecular Recognition" J.Jap.,Soc.D.D.S.4. 7-11 (1989)

[文献書誌] Junzo Sunamoto: "Cell Specificity of Polysaccharide Derivatives on Liposoma Surface" Chem.Lett.in press.

[文献書誌] Akira Nakamura: "Water Sensitivity of a (Fe_4S_4(SR)_4)^1-Cluster and Its Stabilization" Chem.Lett.399-402 (1989)

[文献書誌] Masanobu Hidai: "Catalytic Conversion of Molecular Nitrogen into Silylamines Using Molybdenum and Tungsten Dinitrogen Complexes" J.Am.Chem.Soc.111. 1939-1940 (1989)

[文献書誌] Masanobu Hidai: "Syntheses and Some Reactions of Trimethylsilylated Dinitrogen Complexes of Tungsten and Molybdenum" Bull.Chem.Soc.,Jpn.62. 2953-2959 (1989)

[文献書誌] Masaaki Hirobe: "Highly Efficient Epoxidation of Olefins With Phridine N-Oxides Catalyzed by Ruthenium Porphyrins" Tetrahendron Lett.30. 6545-6548 (1989)

[文献書誌] Yukito Murakami: "pH-Dependent Molecular Recognition Performed by a Polycationic Cubic Azaparacyclophone" Chem.Lett.1199-1202 (1989)

[文献書誌] Shunichi Murahashi: "Flavin-Catalyzed Oxidation of Amines and Sulfur Compounds with Hydrogen Peroxide" J.Am.Chem.Soc.111. 5002-5003 (1989)

[文献書誌] Yoshihiko Morooka: "μ-η^2:η^2=Peroxo Binuclesr Copper Complex,(Cu(HB93,5-i-Pr_2pz)_3)_2(O_2)" J.Am.Chem.Soc.111. 8975-8976 (1989)

[文献書誌] Yoshihiko Morooka: "Formation and Characterization of Mononuclear Acylperoxo Copper(II)Complex" Inorg.Chem.in press.

1989 年度実績報告書

大城芳樹大阪大学, 工学部, 教授 (70028984)