1989 年度実績報告書

光合成機能の光損傷とその回避・修復の分子機構

研究課題

研究課題/領域番号	01304003
研究機関	九州大学
研究代表者	岡山茂樹九州大学, 教養部, 教授 (50038444)
研究分担者	佐藤公行岡山大学, 理学部, 教授 (10032822) 藤田善彦基礎生物学研究所, 教授 (40013560) 浅田浩二京都大学, 食糧科学研究所, 教授 (50027182) 塩井祐三宮崎医科大学, 医学部, 助教授 (70094092) 渡辺正東京大学, 生産技術研究所, 助教授 (70092385)
キーワード	光合成 / 光損傷 / 光化学系 / 反応中心 / クロロフィル / D1タンパク質 / チトクロ-ム / アスコルビン酸ペルオキシダ-ゼ
研究概要	1.各種クロロフィル類とその一連の変性物、誘導体等を迅速・高感度。高分解能で分離できる高速液体クロマトグラフィ-の条件を確立した。それによりクロロフィルとその誘導体の変性の速度定数を各種条件下で定量的に測定した。エチレン処理て誘発されるクロロフィルa分解がフェオフォルバイドaを経由して行われることを証明した。光合成細菌におけるクロロフィル生合成の光条件の検討とアミノレブリン酸合成酵素の抽出・鈍化を行った。 2.光化学系I反応中心での光失活部位が好気条件下ではFe-Sセンタ-、嫌気条件下ではA_0とA_2の間であることが明らかにした。光化学系II反応中心で光損傷をうけ易いD1タンパク質の簡易単離法を確立し、光失活で損傷をうけるアミノ酸残基を明らかにしつつあるが、現在失活初期ではペプチド結合の切断は起こらないことを明らかにしている。さらに、光化学系II反応中心の修復に関与するD1タンパク質のプロセシング酵素を抽出・鈍化し、その性質を調べた。 3.光損傷の回避に関与するアスコルビン酸ペルオキシダ-ゼの分子的性質を明らかにし、自殺基質となる化合物を見いだした。さらに他の光損傷回避機構である光条件に対応するエネルギ-変換系の構成の調節の誘発信号がチトクロ-ムb_6-f複合体の電子状態であることを示すことを明らかにした。

研究成果

(27件)

すべてその他

すべて文献書誌 (27件)

[文献書誌] Tadashi Watanabe Hiroyuki Maeda: "Adsorption-Controlled Redox Activity.Surface-enhanced Raman Investigation of Cystine versus Cysteine on Silver Electrodes" Jouenal of Physical Chemistry. 93. 3258-3260 (1989)
[文献書誌] Masashi Kobayashi H.Maeda,T.Watanabe,H.Nakane,K.Satoh: "Chlorophyll a and β-Carotene Content in the D1/D1/Cytochrome b-559 Reaction Center Complex from Spinach" FEBS Letters. 260. 138-140 (1990)
[文献書誌] Hitoshi Mazaki Tadashi Watanabe: "Epimerization of Chlorophyll Derivatives.4.Self-Catalyzed Epimerization of Chlorophyll a/a' in Organic Solvents" Biochim.Biophys.Acta. (1990)
[文献書誌] 渡辺正、小林正美: "光合成初期過程と電気化学" 電気化学. 57. 652-659 (1989)
[文献書誌] 渡辺正、小林正美: "クロロフィル類の精密分析" 油化学. 38. 876-885 (1989)
[文献書誌] Iba,K.and Takamiya,K: "Action spectra for inhibition by light of accumulation of bacteriochlorophyll and carotenoid during aerobic growth of photosynthetic bacteria" Plant Cell Physiology. 30. 471-477 (1989)
[文献書誌] 藤田善彦、岩間由美、大城香、村上明男、萩原直樹: "Regulation of the size of light-haresting antennae in response to light intensity in the green alga Chlorella phyrenoidosa" Plant Cell Physiology. 30. 1029-1037 (1989)
[文献書誌] 藤田善彦、村上明男、大城香: "Regulation of the stoichiometry of thylakoid components in the photosynthetic system of cyanophytes:model experiments showing that control of the synthesis or supply of Chl a can change the stoichiometric relationship between two photosyatems" Plant Cell Physiology. 31. (1990)
[文献書誌] Siba Prasad Adhikary,村上明男、大城香、藤田善彦: "Photoregulation of respiratory activity in the cyanophyte Synechocystis PCC 6714:A possibility of a simultaneous regulation of content of PS I complex and functional size of respiratory terminal oxidase in thylakoids." Plant Cell Physiology. 31. (1990)
[文献書誌] Terashima,Ichiro,Huang,Lin-Ke and Osmond,C.B.: "Effects of leaf chilling on thylakoid functions,measured at room temperature,in Cucumis sativus L. and Oryza sativa L." Plant and Cell Physiology. 30. 841-850 (1989)
[文献書誌] Shen,Jian-Ren,Terashima,Ichiro and Katoh,Sakae: "The cause for dark,chilling-induced inactivation of photosynthetic oxygen-evolving system in cucumber leaves." Plant Physiology. (1990)
[文献書誌] Terashima,Ichiro and Katoh,Sakae: "A treatment of leaves of Cucumis sativus L. at low temperature in the moderate light causes uncoupling of thylakoids." Plant,Cell and Environment.
[文献書誌] Takahashi,Y. and Satoh,K.: "Identification of the photochemically iodinated amino acid residue on D1-protein in photosystem II core complex by peptide mapping analysis." Biochim.Biophys.Acta. 973. 138-146 (1989)
[文献書誌] Inagaki,N.,Fujita,S.and Satoh,K.: "Solubulization and partial purification of a thylakoidal enzyme of spinach involved in the processin of D1 protein." FEBS Lett.246. 218-222 (1989)
[文献書誌] Fujita,S.,Inagaki,N.,Ono,T.,Inoue,Y.ans Satoh,K.: "Cleavage of a synthetic COOH-terminal oligopeptide of D1 precursor protein by a purified processing enzyme." FEBS Lett.255. 1-4 (1989)
[文献書誌] Takahashi,Y.,Nakane,H.,Kojima,H.and Satoh,K.: "Chromatographycal purification and COOH terminal sequences of photosystem II reaction center proteins D1 and D2." Plant & Cell Physiol.31. (1990)
[文献書誌] Satoh,K.,Hansson,O.and Mathis,P.: "Charge recombination between stabilized P-680^+ and reduced cytochrome b-559 in quinone-reconstituted PSII reaction center." Biochim.Biophys.Acta.
[文献書誌] Kobayashi,M.,Maeda,H.,Watanabe,T.Nakane,H.and Satoh,K.: "Chlorophyll a and β-carotene content in the D1/D2/cytochrome b-559 reaction center complex from spinach." FEBS Lett.260. 138-140 (1990)
[文献書誌] Doi,M.,Shioi,Y.,Morita,M.and Takamiya,K.: "Two types of cytochrome cd_1 in the aerobic photosynthetic bacterium,Erytheobacter sp.OCh 114." Eur.J.Biochem.184. 521-527 (1989)
[文献書誌] Boddi,B.and Shioi,Y.: "Spectroscopic forms of divinyprotochlorophyllide in solid films." Biochim.Biophys.Acta. 1015. 116-120 (1990)
[文献書誌] Shimokawa,K.,Hashizume,A.and Shioi,Y.: "Pheophorbide a as a catabolite of ethylene-induced chlorophyll a degradation." Phytochemistry. (1990)
[文献書誌] Shioi,Y.and Doi,M.: "Aerobic and anaerobic photosynthesis and bacterio-chlorophyll formation in Phodobacter sulfidophilus." Proc.8th International Congress on Photosynthesis. (1990)
[文献書誌] Chen,G-X.and Asada,K.: "Ascorbate peroxidase in tea leaves:Occurrence of two isozymes and their differences in enzymatic and molecular properties" Plant and Cell Physiology. 30. 987-998 (1989)
[文献書誌] Chen,G-X.and Asada,K.: "Hydroxyurea and aminophenol are the suicide inhibitors of ascorbate peroxidase" Journal of Biological Chemistry. 265. 2775-2781 (1990)
[文献書誌] Kanematsu,S.and Asada,K.: "CuZn-Superoxide dismutases in rice:Occurrence of an active,monomeric enzyme and two isozymes in leaf and non-photosynthetic tissues." Plant and Cell Physiology. 30. 381-391 (1989)
[文献書誌] Kanematsu,S.and Asada,K.: "CuZn-Superoxide dismutases from the fern Equisetum arvense and the green alga Spirogyra sp.:Occurrence of chloroplast and cytosol types of enzyme" Plant Cell Physiology. 30. 717-727 (1989)
[文献書誌] Inoue,K.,Fujii,Y.,Yokoyama,E.,Sakurai,H.: "Progress in Photosynthesis Research(ed.M.Baltscheffsky)" Martinus Nijihoff, 4 (1990)

1989 年度 実績報告書

光合成機能の光損傷とその回避・修復の分子機構

研究代表者

岡山 茂樹 九州大学, 教養部, 教授 (50038444)

研究成果

[文献書誌] Tadashi Watanabe Hiroyuki Maeda: "Adsorption-Controlled Redox Activity.Surface-enhanced Raman Investigation of Cystine versus Cysteine on Silver Electrodes" Jouenal of Physical Chemistry. 93. 3258-3260 (1989)

[文献書誌] Masashi Kobayashi H.Maeda,T.Watanabe,H.Nakane,K.Satoh: "Chlorophyll a and β-Carotene Content in the D1/D1/Cytochrome b-559 Reaction Center Complex from Spinach" FEBS Letters. 260. 138-140 (1990)

[文献書誌] Hitoshi Mazaki Tadashi Watanabe: "Epimerization of Chlorophyll Derivatives.4.Self-Catalyzed Epimerization of Chlorophyll a/a' in Organic Solvents" Biochim.Biophys.Acta. (1990)

[文献書誌] 渡辺正、小林正美: "光合成初期過程と電気化学" 電気化学. 57. 652-659 (1989)

[文献書誌] 渡辺正、小林正美: "クロロフィル類の精密分析" 油化学. 38. 876-885 (1989)

[文献書誌] Iba,K.and Takamiya,K: "Action spectra for inhibition by light of accumulation of bacteriochlorophyll and carotenoid during aerobic growth of photosynthetic bacteria" Plant Cell Physiology. 30. 471-477 (1989)

[文献書誌] 藤田善彦、岩間由美、大城香、村上明男、萩原直樹: "Regulation of the size of light-haresting antennae in response to light intensity in the green alga Chlorella phyrenoidosa" Plant Cell Physiology. 30. 1029-1037 (1989)

[文献書誌] Terashima,Ichiro,Huang,Lin-Ke and Osmond,C.B.: "Effects of leaf chilling on thylakoid functions,measured at room temperature,in Cucumis sativus L. and Oryza sativa L." Plant and Cell Physiology. 30. 841-850 (1989)

[文献書誌] Shen,Jian-Ren,Terashima,Ichiro and Katoh,Sakae: "The cause for dark,chilling-induced inactivation of photosynthetic oxygen-evolving system in cucumber leaves." Plant Physiology. (1990)

[文献書誌] Terashima,Ichiro and Katoh,Sakae: "A treatment of leaves of Cucumis sativus L. at low temperature in the moderate light causes uncoupling of thylakoids." Plant,Cell and Environment.

[文献書誌] Takahashi,Y. and Satoh,K.: "Identification of the photochemically iodinated amino acid residue on D1-protein in photosystem II core complex by peptide mapping analysis." Biochim.Biophys.Acta. 973. 138-146 (1989)

[文献書誌] Inagaki,N.,Fujita,S.and Satoh,K.: "Solubulization and partial purification of a thylakoidal enzyme of spinach involved in the processin of D1 protein." FEBS Lett.246. 218-222 (1989)

[文献書誌] Fujita,S.,Inagaki,N.,Ono,T.,Inoue,Y.ans Satoh,K.: "Cleavage of a synthetic COOH-terminal oligopeptide of D1 precursor protein by a purified processing enzyme." FEBS Lett.255. 1-4 (1989)

[文献書誌] Takahashi,Y.,Nakane,H.,Kojima,H.and Satoh,K.: "Chromatographycal purification and COOH terminal sequences of photosystem II reaction center proteins D1 and D2." Plant & Cell Physiol.31. (1990)

[文献書誌] Satoh,K.,Hansson,O.and Mathis,P.: "Charge recombination between stabilized P-680^+ and reduced cytochrome b-559 in quinone-reconstituted PSII reaction center." Biochim.Biophys.Acta.

[文献書誌] Kobayashi,M.,Maeda,H.,Watanabe,T.Nakane,H.and Satoh,K.: "Chlorophyll a and β-carotene content in the D1/D2/cytochrome b-559 reaction center complex from spinach." FEBS Lett.260. 138-140 (1990)

[文献書誌] Doi,M.,Shioi,Y.,Morita,M.and Takamiya,K.: "Two types of cytochrome cd_1 in the aerobic photosynthetic bacterium,Erytheobacter sp.OCh 114." Eur.J.Biochem.184. 521-527 (1989)

[文献書誌] Boddi,B.and Shioi,Y.: "Spectroscopic forms of divinyprotochlorophyllide in solid films." Biochim.Biophys.Acta. 1015. 116-120 (1990)

[文献書誌] Shimokawa,K.,Hashizume,A.and Shioi,Y.: "Pheophorbide a as a catabolite of ethylene-induced chlorophyll a degradation." Phytochemistry. (1990)

[文献書誌] Shioi,Y.and Doi,M.: "Aerobic and anaerobic photosynthesis and bacterio-chlorophyll formation in Phodobacter sulfidophilus." Proc.8th International Congress on Photosynthesis. (1990)

[文献書誌] Chen,G-X.and Asada,K.: "Ascorbate peroxidase in tea leaves:Occurrence of two isozymes and their differences in enzymatic and molecular properties" Plant and Cell Physiology. 30. 987-998 (1989)

[文献書誌] Chen,G-X.and Asada,K.: "Hydroxyurea and aminophenol are the suicide inhibitors of ascorbate peroxidase" Journal of Biological Chemistry. 265. 2775-2781 (1990)

[文献書誌] Kanematsu,S.and Asada,K.: "CuZn-Superoxide dismutases in rice:Occurrence of an active,monomeric enzyme and two isozymes in leaf and non-photosynthetic tissues." Plant and Cell Physiology. 30. 381-391 (1989)

[文献書誌] Kanematsu,S.and Asada,K.: "CuZn-Superoxide dismutases from the fern Equisetum arvense and the green alga Spirogyra sp.:Occurrence of chloroplast and cytosol types of enzyme" Plant Cell Physiology. 30. 717-727 (1989)

[文献書誌] Inoue,K.,Fujii,Y.,Yokoyama,E.,Sakurai,H.: "Progress in Photosynthesis Research(ed.M.Baltscheffsky)" Martinus Nijihoff, 4 (1990)

1989 年度実績報告書

岡山茂樹九州大学, 教養部, 教授 (50038444)