1990 年度実績報告書

真核細胞における鞭毛・繊毛運動の分子機構の解明

研究課題

研究課題/領域番号	01304006
研究機関	東京大学
研究代表者	森沢正昭東京大学, 理学部, 教授 (40013594)
研究分担者	馬場昭次お茶の水女子大学, 理学部, 助教授 (80011691) 林博司名古屋大学, 理学部, 教授 (00022608) 渡辺良雄筑波大学, 生物科学系, 教授 (00015918) 高橋景一東京大学, 理学部, 教授 (40011481) 毛利秀雄東京大学, 教養学部, 教授 (70012268)
キーワード	微小管滑り運動 / 鞭毛・繊毛運動 / 精子運動開始 / 遺伝子クロ-ニング / チュ-ブリン / ダイニン / Ca^<2+>結合タンパク / プロテインキナ-ゼ
研究概要	滑り運動の分子機構に関して高橋は独自に開発した装置で、高Ca^<2+>がウニ精子鞭毛の特定の滑りパタ-ンを高頻度で起すことを示し、上村は10^<ー9>mの精度でウニ精子鞭毛の微小管滑り運動を解析することに成功し、滑りがチュ-ブリンのサイズを反映した振幅で起ることを発見した。神谷はダイニン標品上を微小管を滑らせる実験系を開発し、ダイニンは異なる運動性を起す複数部分からなることを示し、豊島は同様の系で、ダイニンATPaseはキネシン、ミオシンのATPaseに比べ基質特異性が高いこと、加水分解と運動の速度の相関は低いことを示した。毛利はトリプシン分解法でウニの外腕ダイニンβ鎖のATP分解に伴うconformation変化とα鎖の相互作用が滑り運動に重要であることを示し、中村は微小管と微小管結合タンパクの給合を解析し、チュ-ブリンサブユニット間の数個の架橋様式の存在を示した。繊毛運動に関して馬場は、ウニ幼生繊毛に対する神経伝達物質の作用を調べ周期運動のONーOFFと対称性を調節している因子について示し、中岡はゾウリムシで細胞膜の電流変化が繊毛打頻度を調節している事を明らかにした。野口はゾウリムシ繊毛打の逆転がCa^<2+>の膜での流入流出により調節されていることを示した。精子運動開始の分子機構に関し、林はその中核部分がAキナ-ゼ、チロシンキナ-ゼ、15Kタンパクの複合体からなることを示し、又15Kタンパクの抗体を作製した。森沢は海産魚精子の運動開始には細胞内Ca^<2+>、細胞内pHが重要な役割を果していることを明らかにし、奥野は海産無脊椎動物フジツボでCa^<2+>の関与について研究を行った。渡辺はテトラヒメナのカルモジュリンファミリ-に属する3種のCa^<2+>給合タンパクを発見し、更にこれらの遺伝子のクロ-ニングとシ-クエンシングに成功した。又、これらの遺伝子のアンチセンスmRNAを調製し、微量注射法で、これらの結合タンパクの繊毛運動における機能について検討中である。

研究成果
(59件)

すべてその他

すべて文献書誌 (59件)

[文献書誌] Morisawa,M.,Inoda T.& Oda S.: "Reguration of sperm motility by osmotic pressure" Mechanism of Fertilization:Plants to Humans (B.Dale ed.) SpringerーVerlag. H45. 143-154 (1990)
[文献書誌] Morisawa M.& Morisawa S.: "Acquisition and initiation of sperm motility" Controls of Sperm Motility:Biological and Clinical Aspects (C.Gagnom ed) CRC Press. 137-151 (1990)
[文献書誌] Morisawa M.,Oda S.& Inoda T.: "Initiation of sperm motility by osmolality and calcium in teleost and amphibia" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).
[文献書誌] Morisawa S.,Ishida K.& Morisawa M.: "The role of extracellular pH on the acquisition of sperm motility in chum salmon" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).
[文献書誌] K.Ogawa,E.Yokota,S.Wada,M.Okuno & Y.Nakajima: "The outer arm dynein αーheavy chains of sea urchin sperm flagella and emblionic cilia are different." Cell Motility and Cytoskeleton. 16. 58-67 (1990)
[文献書誌] T.Shimizu,M.Okuno,S.P.MarcheseーRagona & K.A.Johnson: "Phosphorothioate analogs of ATP as the substrates of dynein and ciliary or flagellar movement." Europian Journal of Biochemistry. 191. 543-550 (1990)
[文献書誌] Inaba,Kazuo,Ono,Michio & Mohri,Hideo: "Conformational changes of the β chain of the outerーarm dynein from sea urchin sperm flagella coupled with ATP hydrolysis." Journal of Biochemistry. 108. 663-668 (1990)
[文献書誌] Mohri,Hideo,Fujiwara,Akiko,Daumae,Masashi & Mohri,Hideo: "Respiration and motility in starfish spermatozoa at various times in the breeding season." Development,Growth & Differentiation. 32. 375-381 (1990)
[文献書誌] 稲葉一男・毛利秀雄: "鞭毛・繊毛運動の分子機構" 蛋白質核酸酵素. 34. 1505-1512 (1989)
[文献書誌] Inaba,Kazuo,Okuno,Makoto & Mohri,Hideo: "Anthraniloyl ATP,a fluorescent analog of ATP,as a substrate for dynein ATPase and flagellar motility." Archives of Biochemistry and Biophysics. 274. 209-215 (1989)
[文献書誌] Inaba,Kazuo & Mohri,Hideo: "Two states of the conformation of 21S outer arm dynein coupled with ATP hydrolysis." Journal of Biochemistry. 106. 349-354 (1989)
[文献書誌] Inaba,Kazuo & Mohri,Hideo: "Dynamic conformational changes of 21 S dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion." The Journal of Biological Chemistry. 264. 8384-8388 (1989)
[文献書誌] Yamada,A.,N.Ishii,T.Shimmen and K.Takahashi: "MgーATP activity and motility of native thick filaments isolated from the anterior byssus retractor muscle of Mytilus edulis." J.Muscle Res.Cell Motility. 10. 124-134 (1989)
[文献書誌] Abe,O.,T.Yamada,K.Takahashi and H.Sugi: "Initiation of active force development in a molluscan smooth muscle by laser photolysis of caged ATP." Proc.Japan Academy Ser.B. 65. 165-168 (1989)
[文献書誌] Katada,J.,C.Shingyoji and K.Takahashi: "Effects of detergents used to demembranate the seaーurchin spermatozoa on the reactivated flagellar movement,with special reference to the rotatability of the plane of flagellar beat." Cell Struct.Funct.14. 751-758 (1989)
[文献書誌] Yoshimura,K.and K.Takahashi: "Partial extraction of inner dynein arms from an outer armーless mutant of Chlamydomonas reinhadtii." Cell Struct.Funct.14. 741-749 (1989)
[文献書誌] Eshel,D.,C.Shingyoji,K.Yoshimura,B.H.Gibbons,I.R.Gibbons and K.Takahashi: "Transient behavior of sea urchin sperm flagella following an abrupt change in beat frequency." J.exp.Biol.15. 441-451 (1990)
[文献書誌] Yamada,A.,N.Ishii and K.Takahashi: "Direction and speed of actin filaments moving along thick filaments isolated from molluscan smooth muscle." J.Biochem.108. 341-343 (1990)
[文献書誌] Shingyoji,C.,I.R.Gibbons,A.Murakami and K.Takahashi: "Effects of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa." J.exp.Biol.,in press.(1991)
[文献書誌] Shingyoji,C.,J.Katada,K.Takahashi and I.R.Gibbons: "Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea urchin sperm without twisting the axoneme." J.Cell Sci.,in press.(1991)
[文献書誌] Takahashi,K.,C.Shingyoji,J.Katada,D.Eshel and I.R.Gibbons: "Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea urchin spermatozoa." J.Cell Sci.,in press.(1991)
[文献書誌] Ishii,N.,F.Mitsumori and K.Takahashi: "Changes in sarcoplasmic metabolite concentrations and pH associated with the catch contraction and relaxation of the anterior byssus retractor muscle of Mytilus edulis measured by phosphorusー31 nuclear magnetic resonance." J.Muscle Res.Cell Motil.,12,in press.
[文献書誌] Machemer,H.,S.MachemerーRo^^‥hnisch,R Bra^^‥ucker and K.Takahashi: "Gravikinesis in Paramecium:Theory and isolation of a physiological response to the natural gravity vector." J.Comp.Physiol.,in press.
[文献書誌] Ishii,N.,F.Mitsumori,K.Takahashi,A.W.M.Simpson and C.C.Ashley: "Intracellular metabolite and free calcium concentrations during the‘catch'contracation and relaxation in a molluscan smooth muscle." Muscle Energetics(eds R.Paul,G.Elzinga and K.Yamada),Alan R.Liss,NY.463-464 (1989)
[文献書誌] Hayashi H.,Takiguchi K.& HigashiーFujime S.: "Measurement of ATPase activity of immobilized myosin heads" J.Biochem.105. 875-877 (1989)
[文献書誌] Takiguchi K.,Hayashi H Kurimoto E.& HigashiーFujime S.: "In vitro motility of skeletal muscle myosin and its proteolytic fragments" J.Biochem.107. 671-679 (1990)
[文献書誌] 馬場昭次(Ooya et al.): "Swimming behavior of <Paramecium>___ー in the gravity fields:Analysis using gravity as an experimental variable." Proc.6th Space Util.Symp.6. 91-95 (1989)
[文献書誌] 馬場昭次(Ooya et al.): "Analysis of swimming behavior of <Paramecium>___ー under hypergravity conditions." Biol.Sci.Space. 3. 320-321 (1989)
[文献書誌] 馬場昭次: "Threeーdimensional recording and measurement of swimming paths of microーorganisms with two synchronized monochrome cameras." Rev.Sci.Instrum.1-2 (1991)
[文献書誌] 馬場昭次(Mogami et al.): "Five different states of ciliary activity in the epaulette of echinoplutei." J.Exp.Biol.155. 1-11 (1991)
[文献書誌] 馬場昭次: "Discrete nature of flagellar bending detected by digital image analysis." Biomathematics. 1-10 (1991)
[文献書誌] 馬場昭次: "Highーresolution analysis of flagellar bends." Comparative Spermatology. (1991)
[文献書誌] Takemasa,T.,Takagi,T.,Kobayashi,T.,Konishi,K.,& Watanabe,Y.: "The third calmodulin family protein in <Tetrahymena>___ー:Cloning of the gene for <Tetrahymena>___ー calciumーbinding protein of 23kDa (TCBPー23)" Journal of Biological Chemistry. 265. 2514-2517 (1990)
[文献書誌] Watanabe,Y.,Ohba,H.,Hirono,M.,& Yasuda,T.: "Analysis of furrow formation and furrowing during cell division in <Tetrahymena>___ー using cellーdivisionーarrest mutants" Annals of The New York Academy of Sciences. 582. 166-178 (1990)
[文献書誌] Edamatsu,M.,Hirono,M.,Watanabe,Y.: "Purification and characterization of <Tetrahymena>___ー profilin" Biophysica Biochimica Research Communication. 170. 957-962 (1990)
[文献書誌] Watanabe,Y.,Hirono,M.,Takemasa,T.,& Numata,O.: "Protozoan genes:As viewed from <Tetrahymena>___ー genes" Japanese Journal of Protozoology. 23. 1-11 (1990)
[文献書誌] Hirono,M.,Tanaka,R.,& Watanabe,Y.: "<Tetrahymena>___ー actin:Copolymerization with skeletal muscle actin and interactions with muscle actinーbinding proteins" Journal of Biochemistry. 107. 32-36 (1990)
[文献書誌] Numata,O.,Tomiyoshi,T.Kurasawa,Y.,Fu,Zhen xing,Takahashi,M.,Kosaka,T.Chiba,J.,& watanabe,Y.: "Antibodies against <Tetrahymena>___ー 14ーnm filament forming protein recogniz replication bund in <Euplotes>___ー" Experimental Cell Research. 193. (1991)
[文献書誌] Kagami,O.and Kamiya,R.: "Strikingly low ATPase in flagellar axonemes of a Chlamydomonas mutant missing outer dynein arms." European Journal of Biochemistry. 187. 441-446 (1990)
[文献書誌] Kagami,O.,Takada,S.and Kamiya R.: "Microtubule translocation caused by three subspecies of innerーarm dynein from Chlamydomonas flagella." FEBS Letters. 264. 179-182 (1990)
[文献書誌] Kamiya,R.,Kurimoto,E.and Muto,E.: "Two types of flagellar mutants missing different components of innerーarm dynein." Journal of Cell Biology. (1991)
[文献書誌] Nakamura,K.,Masuyama,E.: "Characterization of an Axonemal Protein from Tetrahymena cilia." Bull.Hiroshima Women's Univ.25. 21-28 (1989)
[文献書誌] Nakamura,K.,Masuyama,E.Wada,S.,and Okuno,M.: "Application of Stainsーall Staining to the Analysis of Axonemal tubulins." J.Biochem.Biophys.Methods. 21. 237-245 (1990)
[文献書誌] Nakamura,K.,Furukohri,T.Shikama,K.: "A Computer Display of the Free Energy Changes of ATP Hydrolysis." Biochem.Education. (1991)
[文献書誌] Y.Nakaoka & H.Machemer: "Effects of cyclic nucleotides and intracellular Ca on voltageーactivated ciliary beating in <Paramecium>___ー" Journal of Comparative Physiology. 166. 401-406 (1990)
[文献書誌] T.Inoue & Y.Nakaoka: "Coldーsensitive responses in the <Paramecium>___ー membrane" Cell Structure and Function. 15. 107-112 (1990)
[文献書誌] R.Tokioka,K.Matsuoka,Y.Nakaoka & Y.Kito: "Extraction of retinal from <Paramecium>___ー <bursaria>___ー" Photochemistry and Photobiology. 53. 149-151 (1991)
[文献書誌] Y.Nakaoka,R.Tokioka,T.Shinozawa,J.Fujita & J.Usukura: "Photoreception of <Paramecium>___ー cilia:Localization of photosensitivity and binding with antiーfrog rhodopsin IgG" Journal of Cell Science. (1991)
[文献書誌] Shinji Kamimura and Ritsu Kamiya: "Highーfrequency nanometerーscale vibration in“quiescent"flagellar axonemes." Nature. 340. 476-478 (1989)
[文献書誌] M.Noguchi,Y.Nakamura and K.Okamoto: "Control of the ciliary orientation on the ciliated sheets of <Paramecium>___ーーーDifferential distribution of sensitivity to cyclic nucleotides." Submitted to Cell Motil.Cytoskeleton.
[文献書誌] Toyoshima,Y.Y.,Kron,S.J.& Spudich,J.A.: "The myosin step size:Measurement of the unit displacement per ATP hydrolysed in an in vitro assay." Proc.Natl.Acad.Sci.87. 7130-7134 (1990)
[文献書誌] Schwyter,D.H.,Kron,S.J.,Toyoshima,Y.Y.,Spudich,J.A.& Reisler,E.: "Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin." J.Cell Biol.111. 465-470 (1990)
[文献書誌] 豊島陽子,毛利秀雄: "微小管関連モ-タ-タンパク質" 比較生理生化学. 7. 71-79 (1990)
[文献書誌] Toyoshima,Y.Y.: "Flexibility in actinーmyosin motility system revealed by in vitro motility assay." Adv.Biophys.(1990)
[文献書誌] Toyoshima,Y.Y.: "Bidirectional movement of actin filaments along tracks of heavy meromyosin and native thick filaments." J.Cell Sci.(1991)
[文献書誌] Shimizu,T.,Furusawa,K.,Ohashi S.,Toyoshima,Y.Y.,Okuno,M.,Malik,F.& Vale R.D.: "Nucleotide specificity of the enzymatic and motile activities of dynein,kinesin and heavy meromyosin." J.Cell Biol.(1991)
[文献書誌] Watanabe,Y.,HiranoーOhnishi,J.,& Takemasa,T.: "Calciumーbinding proteins and ciliary movement regulation in <Tetrahymena>___ー,Chaptor 19 in Calcium as an Intracellular Messenger in Eucaryotic Microbes,edited by D.h.O'Day" Ammerican Society for Microbiology Washington,D.C., 346-361 (1990)
[文献書誌] Takemasa,T.,Takagi,T.,& Watanabe,Y.: "Calciumーbinding protein from <Tetrahymena>___ー,In Novel CalciumーBinding Proteins.Fundamentals and Clinical Implications,edited by C.Heizmann" Springer Press, (1991)
[文献書誌] Kron,S.J.,Toyoshima,Y.Y.,Uyeda,T.Q.P.& Spudich,J.A.: "Assays for actin sliding movement over myosin coated surfaces.In“Methods in Enzymology"" Academic Press, (1990)

1990 年度 実績報告書

真核細胞における鞭毛・繊毛運動の分子機構の解明

研究代表者

森沢 正昭 東京大学, 理学部, 教授 (40013594)

研究成果

[文献書誌] Morisawa,M.,Inoda T.& Oda S.: "Reguration of sperm motility by osmotic pressure" Mechanism of Fertilization:Plants to Humans (B.Dale ed.) SpringerーVerlag. H45. 143-154 (1990)

[文献書誌] Morisawa M.& Morisawa S.: "Acquisition and initiation of sperm motility" Controls of Sperm Motility:Biological and Clinical Aspects (C.Gagnom ed) CRC Press. 137-151 (1990)

[文献書誌] Morisawa M.,Oda S.& Inoda T.: "Initiation of sperm motility by osmolality and calcium in teleost and amphibia" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).

[文献書誌] Morisawa S.,Ishida K.& Morisawa M.: "The role of extracellular pH on the acquisition of sperm motility in chum salmon" Proceedings of the VIth International Congress on Spermatology:Comparative Spermatology (Siena Italy).

[文献書誌] K.Ogawa,E.Yokota,S.Wada,M.Okuno & Y.Nakajima: "The outer arm dynein αーheavy chains of sea urchin sperm flagella and emblionic cilia are different." Cell Motility and Cytoskeleton. 16. 58-67 (1990)

[文献書誌] T.Shimizu,M.Okuno,S.P.MarcheseーRagona & K.A.Johnson: "Phosphorothioate analogs of ATP as the substrates of dynein and ciliary or flagellar movement." Europian Journal of Biochemistry. 191. 543-550 (1990)

[文献書誌] Inaba,Kazuo,Ono,Michio & Mohri,Hideo: "Conformational changes of the β chain of the outerーarm dynein from sea urchin sperm flagella coupled with ATP hydrolysis." Journal of Biochemistry. 108. 663-668 (1990)

[文献書誌] Mohri,Hideo,Fujiwara,Akiko,Daumae,Masashi & Mohri,Hideo: "Respiration and motility in starfish spermatozoa at various times in the breeding season." Development,Growth & Differentiation. 32. 375-381 (1990)

[文献書誌] 稲葉 一男・毛利 秀雄: "鞭毛・繊毛運動の分子機構" 蛋白質 核酸 酵素. 34. 1505-1512 (1989)

[文献書誌] Inaba,Kazuo,Okuno,Makoto & Mohri,Hideo: "Anthraniloyl ATP,a fluorescent analog of ATP,as a substrate for dynein ATPase and flagellar motility." Archives of Biochemistry and Biophysics. 274. 209-215 (1989)

[文献書誌] Inaba,Kazuo & Mohri,Hideo: "Two states of the conformation of 21S outer arm dynein coupled with ATP hydrolysis." Journal of Biochemistry. 106. 349-354 (1989)

[文献書誌] Inaba,Kazuo & Mohri,Hideo: "Dynamic conformational changes of 21 S dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion." The Journal of Biological Chemistry. 264. 8384-8388 (1989)

[文献書誌] Yamada,A.,N.Ishii,T.Shimmen and K.Takahashi: "MgーATP activity and motility of native thick filaments isolated from the anterior byssus retractor muscle of Mytilus edulis." J.Muscle Res.Cell Motility. 10. 124-134 (1989)

[文献書誌] Abe,O.,T.Yamada,K.Takahashi and H.Sugi: "Initiation of active force development in a molluscan smooth muscle by laser photolysis of caged ATP." Proc.Japan Academy Ser.B. 65. 165-168 (1989)

[文献書誌] Katada,J.,C.Shingyoji and K.Takahashi: "Effects of detergents used to demembranate the seaーurchin spermatozoa on the reactivated flagellar movement,with special reference to the rotatability of the plane of flagellar beat." Cell Struct.Funct.14. 751-758 (1989)

[文献書誌] Yoshimura,K.and K.Takahashi: "Partial extraction of inner dynein arms from an outer armーless mutant of Chlamydomonas reinhadtii." Cell Struct.Funct.14. 741-749 (1989)

[文献書誌] Eshel,D.,C.Shingyoji,K.Yoshimura,B.H.Gibbons,I.R.Gibbons and K.Takahashi: "Transient behavior of sea urchin sperm flagella following an abrupt change in beat frequency." J.exp.Biol.15. 441-451 (1990)

[文献書誌] Yamada,A.,N.Ishii and K.Takahashi: "Direction and speed of actin filaments moving along thick filaments isolated from molluscan smooth muscle." J.Biochem.108. 341-343 (1990)

[文献書誌] Shingyoji,C.,I.R.Gibbons,A.Murakami and K.Takahashi: "Effects of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa." J.exp.Biol.,in press.(1991)

[文献書誌] Shingyoji,C.,J.Katada,K.Takahashi and I.R.Gibbons: "Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea urchin sperm without twisting the axoneme." J.Cell Sci.,in press.(1991)

[文献書誌] Takahashi,K.,C.Shingyoji,J.Katada,D.Eshel and I.R.Gibbons: "Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea urchin spermatozoa." J.Cell Sci.,in press.(1991)

[文献書誌] Machemer,H.,S.MachemerーRo^^‥hnisch,R Bra^^‥ucker and K.Takahashi: "Gravikinesis in Paramecium:Theory and isolation of a physiological response to the natural gravity vector." J.Comp.Physiol.,in press.

[文献書誌] Ishii,N.,F.Mitsumori,K.Takahashi,A.W.M.Simpson and C.C.Ashley: "Intracellular metabolite and free calcium concentrations during the‘catch'contracation and relaxation in a molluscan smooth muscle." Muscle Energetics(eds R.Paul,G.Elzinga and K.Yamada),Alan R.Liss,NY.463-464 (1989)

[文献書誌] Hayashi H.,Takiguchi K.& HigashiーFujime S.: "Measurement of ATPase activity of immobilized myosin heads" J.Biochem.105. 875-877 (1989)

[文献書誌] Takiguchi K.,Hayashi H Kurimoto E.& HigashiーFujime S.: "In vitro motility of skeletal muscle myosin and its proteolytic fragments" J.Biochem.107. 671-679 (1990)

[文献書誌] 馬場 昭次(Ooya et al.): "Swimming behavior of <Paramecium>___ー in the gravity fields:Analysis using gravity as an experimental variable." Proc.6th Space Util.Symp.6. 91-95 (1989)

[文献書誌] 馬場 昭次(Ooya et al.): "Analysis of swimming behavior of <Paramecium>___ー under hypergravity conditions." Biol.Sci.Space. 3. 320-321 (1989)

[文献書誌] 馬場 昭次: "Threeーdimensional recording and measurement of swimming paths of microーorganisms with two synchronized monochrome cameras." Rev.Sci.Instrum.1-2 (1991)

[文献書誌] 馬場 昭次(Mogami et al.): "Five different states of ciliary activity in the epaulette of echinoplutei." J.Exp.Biol.155. 1-11 (1991)

[文献書誌] 馬場 昭次: "Discrete nature of flagellar bending detected by digital image analysis." Biomathematics. 1-10 (1991)

[文献書誌] 馬場 昭次: "Highーresolution analysis of flagellar bends." Comparative Spermatology. (1991)

[文献書誌] Takemasa,T.,Takagi,T.,Kobayashi,T.,Konishi,K.,& Watanabe,Y.: "The third calmodulin family protein in <Tetrahymena>___ー:Cloning of the gene for <Tetrahymena>___ー calciumーbinding protein of 23kDa (TCBPー23)" Journal of Biological Chemistry. 265. 2514-2517 (1990)

[文献書誌] Watanabe,Y.,Ohba,H.,Hirono,M.,& Yasuda,T.: "Analysis of furrow formation and furrowing during cell division in <Tetrahymena>___ー using cellーdivisionーarrest mutants" Annals of The New York Academy of Sciences. 582. 166-178 (1990)

[文献書誌] Edamatsu,M.,Hirono,M.,Watanabe,Y.: "Purification and characterization of <Tetrahymena>___ー profilin" Biophysica Biochimica Research Communication. 170. 957-962 (1990)

[文献書誌] Watanabe,Y.,Hirono,M.,Takemasa,T.,& Numata,O.: "Protozoan genes:As viewed from <Tetrahymena>___ー genes" Japanese Journal of Protozoology. 23. 1-11 (1990)

[文献書誌] Hirono,M.,Tanaka,R.,& Watanabe,Y.: "<Tetrahymena>___ー actin:Copolymerization with skeletal muscle actin and interactions with muscle actinーbinding proteins" Journal of Biochemistry. 107. 32-36 (1990)

[文献書誌] Numata,O.,Tomiyoshi,T.Kurasawa,Y.,Fu,Zhen xing,Takahashi,M.,Kosaka,T.Chiba,J.,& watanabe,Y.: "Antibodies against <Tetrahymena>___ー 14ーnm filament forming protein recogniz replication bund in <Euplotes>___ー" Experimental Cell Research. 193. (1991)

[文献書誌] Kagami,O.and Kamiya,R.: "Strikingly low ATPase in flagellar axonemes of a Chlamydomonas mutant missing outer dynein arms." European Journal of Biochemistry. 187. 441-446 (1990)

[文献書誌] Kagami,O.,Takada,S.and Kamiya R.: "Microtubule translocation caused by three subspecies of innerーarm dynein from Chlamydomonas flagella." FEBS Letters. 264. 179-182 (1990)

[文献書誌] Kamiya,R.,Kurimoto,E.and Muto,E.: "Two types of flagellar mutants missing different components of innerーarm dynein." Journal of Cell Biology. (1991)

[文献書誌] Nakamura,K.,Masuyama,E.: "Characterization of an Axonemal Protein from Tetrahymena cilia." Bull.Hiroshima Women's Univ.25. 21-28 (1989)

[文献書誌] Nakamura,K.,Masuyama,E.Wada,S.,and Okuno,M.: "Application of Stainsーall Staining to the Analysis of Axonemal tubulins." J.Biochem.Biophys.Methods. 21. 237-245 (1990)

[文献書誌] Nakamura,K.,Furukohri,T.Shikama,K.: "A Computer Display of the Free Energy Changes of ATP Hydrolysis." Biochem.Education. (1991)

[文献書誌] Y.Nakaoka & H.Machemer: "Effects of cyclic nucleotides and intracellular Ca on voltageーactivated ciliary beating in <Paramecium>___ー" Journal of Comparative Physiology. 166. 401-406 (1990)

[文献書誌] T.Inoue & Y.Nakaoka: "Coldーsensitive responses in the <Paramecium>___ー membrane" Cell Structure and Function. 15. 107-112 (1990)

[文献書誌] R.Tokioka,K.Matsuoka,Y.Nakaoka & Y.Kito: "Extraction of retinal from <Paramecium>___ー <bursaria>___ー" Photochemistry and Photobiology. 53. 149-151 (1991)

[文献書誌] Y.Nakaoka,R.Tokioka,T.Shinozawa,J.Fujita & J.Usukura: "Photoreception of <Paramecium>___ー cilia:Localization of photosensitivity and binding with antiーfrog rhodopsin IgG" Journal of Cell Science. (1991)

[文献書誌] Shinji Kamimura and Ritsu Kamiya: "Highーfrequency nanometerーscale vibration in“quiescent"flagellar axonemes." Nature. 340. 476-478 (1989)

[文献書誌] M.Noguchi,Y.Nakamura and K.Okamoto: "Control of the ciliary orientation on the ciliated sheets of <Paramecium>___ーーーDifferential distribution of sensitivity to cyclic nucleotides." Submitted to Cell Motil.Cytoskeleton.

[文献書誌] Toyoshima,Y.Y.,Kron,S.J.& Spudich,J.A.: "The myosin step size:Measurement of the unit displacement per ATP hydrolysed in an in vitro assay." Proc.Natl.Acad.Sci.87. 7130-7134 (1990)

[文献書誌] Schwyter,D.H.,Kron,S.J.,Toyoshima,Y.Y.,Spudich,J.A.& Reisler,E.: "Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin." J.Cell Biol.111. 465-470 (1990)

[文献書誌] 豊島 陽子,毛利 秀雄: "微小管関連モ-タ-タンパク質" 比較生理生化学. 7. 71-79 (1990)

[文献書誌] Toyoshima,Y.Y.: "Flexibility in actinーmyosin motility system revealed by in vitro motility assay." Adv.Biophys.(1990)

[文献書誌] Toyoshima,Y.Y.: "Bidirectional movement of actin filaments along tracks of heavy meromyosin and native thick filaments." J.Cell Sci.(1991)

[文献書誌] Shimizu,T.,Furusawa,K.,Ohashi S.,Toyoshima,Y.Y.,Okuno,M.,Malik,F.& Vale R.D.: "Nucleotide specificity of the enzymatic and motile activities of dynein,kinesin and heavy meromyosin." J.Cell Biol.(1991)

[文献書誌] Takemasa,T.,Takagi,T.,& Watanabe,Y.: "Calciumーbinding protein from <Tetrahymena>___ー,In Novel CalciumーBinding Proteins.Fundamentals and Clinical Implications,edited by C.Heizmann" Springer Press, (1991)

[文献書誌] Kron,S.J.,Toyoshima,Y.Y.,Uyeda,T.Q.P.& Spudich,J.A.: "Assays for actin sliding movement over myosin coated surfaces.In“Methods in Enzymology"" Academic Press, (1990)

1990 年度実績報告書

森沢正昭東京大学, 理学部, 教授 (40013594)

[文献書誌] 稲葉一男・毛利秀雄: "鞭毛・繊毛運動の分子機構" 蛋白質核酸酵素. 34. 1505-1512 (1989)

[文献書誌] 馬場昭次(Ooya et al.): "Swimming behavior of <Paramecium>___ー in the gravity fields:Analysis using gravity as an experimental variable." Proc.6th Space Util.Symp.6. 91-95 (1989)

[文献書誌] 馬場昭次(Ooya et al.): "Analysis of swimming behavior of <Paramecium>___ー under hypergravity conditions." Biol.Sci.Space. 3. 320-321 (1989)

[文献書誌] 馬場昭次: "Threeーdimensional recording and measurement of swimming paths of microーorganisms with two synchronized monochrome cameras." Rev.Sci.Instrum.1-2 (1991)

[文献書誌] 馬場昭次(Mogami et al.): "Five different states of ciliary activity in the epaulette of echinoplutei." J.Exp.Biol.155. 1-11 (1991)

[文献書誌] 馬場昭次: "Discrete nature of flagellar bending detected by digital image analysis." Biomathematics. 1-10 (1991)

[文献書誌] 馬場昭次: "Highーresolution analysis of flagellar bends." Comparative Spermatology. (1991)

[文献書誌] Takemasa,T.,Takagi,T.,Kobayashi,T.,Konishi,K.,& Watanabe,Y.: "The third calmodulin family protein in <Tetrahymena>_ー:Cloning of the gene for <Tetrahymena>_ー calciumーbinding protein of 23kDa (TCBPー23)" Journal of Biological Chemistry. 265. 2514-2517 (1990)

[文献書誌] Numata,O.,Tomiyoshi,T.Kurasawa,Y.,Fu,Zhen xing,Takahashi,M.,Kosaka,T.Chiba,J.,& watanabe,Y.: "Antibodies against <Tetrahymena>_ー 14ーnm filament forming protein recogniz replication bund in <Euplotes>_ー" Experimental Cell Research. 193. (1991)

[文献書誌] R.Tokioka,K.Matsuoka,Y.Nakaoka & Y.Kito: "Extraction of retinal from <Paramecium>_ー <bursaria>_ー" Photochemistry and Photobiology. 53. 149-151 (1991)

[文献書誌] 豊島陽子,毛利秀雄: "微小管関連モ-タ-タンパク質" 比較生理生化学. 7. 71-79 (1990)