1989 年度実績報告書

Glycobiologyの基礎的研究

研究課題

研究課題/領域番号	01304057
研究機関	東京大学
研究代表者	木幡陽東京大学, 医科学研究所, 教授 (30030852)
研究分担者	大沢利昭東京大学, 薬学部, 教授 (40012603) 小川智也理化学研究所, 主任研究員 (30087572) 遠藤正彦弘前大学, 医学部, 教授 (20004616) 井上圭三東京大学, 薬学学部, 教授 (30072937) 稲垣冬彦東京都臨床医学総合研究所, 室長 (70011757)
キーワード	糖タンパク質 / 糖脂質 / プロテオグリカン / エンドグリコシダ-ゼ / レクチン / モノクロ-ン抗体 / 糖転移酵素 / エリスロポエチン
研究概要	1)複合糖質糖鎖の微量構造分析法の開発稲垣は様々な糖脂質に対するNMRのデ-タを蓄積し、又新しくralayed HOHAHAという技術を開発した。遠藤はプロテオグリカンの糖鎖の根元附近を切断する3種のエンドグリコシダ-ゼを、又堀田はムチン糖類のタンパク質との結合部位を水解する酸素を発見して部分精製した。山科は糖鎖と反応するモノクロ-ン抗体を作成し、又山下は各種レクチンを不溶化したカラムの糖結合性を詳細に調べて、糖類の構造解析に有効に使われることを示した。これらの酵素や糖鎖結合タンパク質は本研究の遂行上きわめて有力な手段となろう。 2)鎖鎖の合成法の確立小川はこれまでほとんど手のつけられていなかったbisecting GlcNAcと根元にフコ-スを持つ複合型Asn結合糖鎖群の化学合成法を確立した。鈴木はマウスのGM4,GM1ならびにGM2などの糖脂質の発現に関与する遺伝子群を同定し、それぞれGs1-4、Gs1-7,Ggm-2と名付けた。又,牧田はブタのA型並びにH型抗原糖脂質の生合成機構を明らかにした。これらの遺伝子の産物は今後糖類の酵素合成に有用となろう。 3)複合糖質糖鎖の機能研究糖タンパク質領域ではマウス線維芽細胞の増殖と悪性化に関与する糖鎖の解析(井上)、リソソ-ム膜糖タンパク質の特徴(加藤)、細胞接着因子ビトロネクチンの糖鎖の解析(瀬野)などが進展した。細胞表面認識に関しては、表面レセプタ-としてのガラクト-ス転移酵素の局在(村松)、エリスロポエチンの生理活性発現における糖鎖構造の重要性(木幡)などが示された。又糖脂質領域では神経の発生に伴う糖鎖パタ-ンの変動(平林)、神経突起形成へのGQ1bの作用機構の解明(永井)などが著明な成果を挙げた。

研究成果
(19件)

すべてその他

すべて文献書誌 (19件)

[文献書誌] Takeuchi,M.,Inoue,N.,Strickland,T.W.,Kubota,M.,Kobata,A.: "Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells." Proc.Natl.Acad.Sci.,U.S.86. 7819-7822 (1989)
[文献書誌] Iwamori,M.,Kawaguchi,T.,Nagai,Y.: "Differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase." J.Biochem.105. 723-727 (1989)
[文献書誌] Kawano,M.,Honke,K.,Tachi,M.,Gasa,S.,Makita,A.: "An assay method for ganglioside synthase using anion-exchanger chromatography." Anal.Biochem.182. 9-15 (1989)
[文献書誌] Ogawa,H.K.,Yatohgo,T.,Izumi,M.,Hayashi,M.,Seno,N.: "Diversities in amimal vitronectins.Differences in molecular weight,immunoreactivity and carbohydrate chains." Biochim.Biophys.Acta.
[文献書誌] Hirabayashi,Y.,Hirota,M.,Suzuki,Y.,Matsumoto,M.,Obata,K.,Ando,S.: "Developmentally expressed O-acetyl ganglioside GT3 in fetal rat cerebral cortex." Neuroscience Lett.106. 193-198 (1989)
[文献書誌] Inagaki,F.,Shimada,I.,Kohda,D.,Suzuki,A.,Box,A.: "Relayed HOHAHA,a useful method for extracting subspectra of individual components of sugar chains." J.Magnetic Resonance. 81. 186-190 (1989)
[文献書誌] Yamashita,Y.,Imai,Y.,Osawa,T.: "α-2,3-Linked sialic acids are more abundant in CD45 antigens and leukosialins of abnormal T cells of 1pr mice than those of normal T cels." J.Biochem.106. 961-965 (1989)
[文献書誌] Nakamura,K.,Hashimoto,Y.,Moriwaki,K.,Yamakawa,T.,Suzuki,A.: "Genetic regulation of GM4(NeuAc)expression in mouse erthrocytes." J.Biochem.107. 3-7 (1990)
[文献書誌] Furuno,K.,Ishikawa,T.,Akasaki,K.,Yano,S.,Kato,K.: "Morphological localization of a major lysosomal membrane glycoprotein in the endocytic membrane system." J.Biochem.106. 708-716 (1989)
[文献書誌] Shimada,E.,Matsumura,S.: "Determination of stokes radii of hyaluronate oligosaccharides by Sephadex chromatography." J.Chromatogr.472. 145-154 (1989)
[文献書誌] Yamagata,M.,Suzuki,S.,Akiyama,S.K.,Yamada,K.M.,Kimata,K.: "Regulation of cell-substrate adhesion by proteoglycans immobilized on extracellular substrates." J.Biol.Chem. 264. 8012-8018 (1989)
[文献書誌] Yamamoto,S.,Hase,S.,Yamauchi,H.,Tanimoto,T.Ikenaka,T.: "Studies on the sugar chains of interferon-γ from human peripheral-blood lymphocytes." J.Biochem.105. 1034-1039 (1989)
[文献書誌] Yamazaki,F.,Nukada,T.,Ito,Y.,Sato,S.,Ogawa,T.: "Stereoselective synthesis of a core glycoheptaose of bisected biantennary complex type glycan of glycoproteins." Tetrahedron Lett.30. 4417-4420 (1989)
[文献書誌] Yamashita,K.,Kobata,A.,Suzuki,T.,Umetsu,K.: "Allomyrina dichotoma lectins." Methods Enzymol.179. 331-341 (1989)
[文献書誌] Watanabe,S.,Umeda,M.,Nomura,T.,Kobayashi,T.,Inoue,K.: "Mitogenic response of near-diploid mouse cell line m5S/1M induced by epidermal growth factor." J.Cellu.Physiol.(1990)
[文献書誌] Goso,Y.,and Hotta,K.: "Type of oligosaccharide sulphation,depending on mucus glycoprotein source,corpus or antral,in rat stomach." Biochem.J.264. 805-812 (1989)
[文献書誌] Takagi,K.,Kon,A.,Kawasaki,H.,Nakamura,T.,Endo,M.: "Preparation and application of a fluorogenic substrate for endoーβ-xylosidase." J.Biochem.Biophys.Methods. 19. 207-214 (1989)
[文献書誌] Muramatsu,H.,Muramatsu,T.: "Analysis of glycoprotein-bound carbohydrates from pluripotent embryonal carcinoma cells by poke weed agglutinin-agarose." J.Biochem. (1990)
[文献書誌] Kitagawa,H,.Nakada,H.,Kurosaka,A.,Hiraiwa,N.,Yamashina,I.: "Three novel ologosaccharides with the sialyl-Le^a structure in human milk." Biochemistry. 28. 8891-8897 (1989)

1989 年度 実績報告書

Glycobiologyの基礎的研究

研究代表者

木幡 陽 東京大学, 医科学研究所, 教授 (30030852)

研究成果

[文献書誌] Takeuchi,M.,Inoue,N.,Strickland,T.W.,Kubota,M.,Kobata,A.: "Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells." Proc.Natl.Acad.Sci.,U.S.86. 7819-7822 (1989)

[文献書誌] Iwamori,M.,Kawaguchi,T.,Nagai,Y.: "Differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase." J.Biochem.105. 723-727 (1989)

[文献書誌] Kawano,M.,Honke,K.,Tachi,M.,Gasa,S.,Makita,A.: "An assay method for ganglioside synthase using anion-exchanger chromatography." Anal.Biochem.182. 9-15 (1989)

[文献書誌] Ogawa,H.K.,Yatohgo,T.,Izumi,M.,Hayashi,M.,Seno,N.: "Diversities in amimal vitronectins.Differences in molecular weight,immunoreactivity and carbohydrate chains." Biochim.Biophys.Acta.

[文献書誌] Hirabayashi,Y.,Hirota,M.,Suzuki,Y.,Matsumoto,M.,Obata,K.,Ando,S.: "Developmentally expressed O-acetyl ganglioside GT3 in fetal rat cerebral cortex." Neuroscience Lett.106. 193-198 (1989)

[文献書誌] Inagaki,F.,Shimada,I.,Kohda,D.,Suzuki,A.,Box,A.: "Relayed HOHAHA,a useful method for extracting subspectra of individual components of sugar chains." J.Magnetic Resonance. 81. 186-190 (1989)

[文献書誌] Yamashita,Y.,Imai,Y.,Osawa,T.: "α-2,3-Linked sialic acids are more abundant in CD45 antigens and leukosialins of abnormal T cells of 1pr mice than those of normal T cels." J.Biochem.106. 961-965 (1989)

[文献書誌] Nakamura,K.,Hashimoto,Y.,Moriwaki,K.,Yamakawa,T.,Suzuki,A.: "Genetic regulation of GM4(NeuAc)expression in mouse erthrocytes." J.Biochem.107. 3-7 (1990)

[文献書誌] Furuno,K.,Ishikawa,T.,Akasaki,K.,Yano,S.,Kato,K.: "Morphological localization of a major lysosomal membrane glycoprotein in the endocytic membrane system." J.Biochem.106. 708-716 (1989)

[文献書誌] Shimada,E.,Matsumura,S.: "Determination of stokes radii of hyaluronate oligosaccharides by Sephadex chromatography." J.Chromatogr.472. 145-154 (1989)

[文献書誌] Yamagata,M.,Suzuki,S.,Akiyama,S.K.,Yamada,K.M.,Kimata,K.: "Regulation of cell-substrate adhesion by proteoglycans immobilized on extracellular substrates." J.Biol.Chem. 264. 8012-8018 (1989)

[文献書誌] Yamamoto,S.,Hase,S.,Yamauchi,H.,Tanimoto,T.Ikenaka,T.: "Studies on the sugar chains of interferon-γ from human peripheral-blood lymphocytes." J.Biochem.105. 1034-1039 (1989)

[文献書誌] Yamazaki,F.,Nukada,T.,Ito,Y.,Sato,S.,Ogawa,T.: "Stereoselective synthesis of a core glycoheptaose of bisected biantennary complex type glycan of glycoproteins." Tetrahedron Lett.30. 4417-4420 (1989)

[文献書誌] Yamashita,K.,Kobata,A.,Suzuki,T.,Umetsu,K.: "Allomyrina dichotoma lectins." Methods Enzymol.179. 331-341 (1989)

[文献書誌] Watanabe,S.,Umeda,M.,Nomura,T.,Kobayashi,T.,Inoue,K.: "Mitogenic response of near-diploid mouse cell line m5S/1M induced by epidermal growth factor." J.Cellu.Physiol.(1990)

[文献書誌] Goso,Y.,and Hotta,K.: "Type of oligosaccharide sulphation,depending on mucus glycoprotein source,corpus or antral,in rat stomach." Biochem.J.264. 805-812 (1989)

[文献書誌] Takagi,K.,Kon,A.,Kawasaki,H.,Nakamura,T.,Endo,M.: "Preparation and application of a fluorogenic substrate for endoーβ-xylosidase." J.Biochem.Biophys.Methods. 19. 207-214 (1989)

[文献書誌] Muramatsu,H.,Muramatsu,T.: "Analysis of glycoprotein-bound carbohydrates from pluripotent embryonal carcinoma cells by poke weed agglutinin-agarose." J.Biochem. (1990)

[文献書誌] Kitagawa,H,.Nakada,H.,Kurosaka,A.,Hiraiwa,N.,Yamashina,I.: "Three novel ologosaccharides with the sialyl-Le^a structure in human milk." Biochemistry. 28. 8891-8897 (1989)

1989 年度実績報告書

木幡陽東京大学, 医科学研究所, 教授 (30030852)