1990 年度実績報告書

増殖因子受容体およびリンパ球活性化調節因子としてのヒトIgEーFcレセプタ-解析

研究課題

研究課題/領域番号	01480188
研究機関	京都大学
研究代表者	淀井淳司京都大学, ウィルス研究所, 教授 (80108993)
キーワード	低親和性FcεRII / CD23 / チロシン燐酸化酵素 / EBV / リュウマチ
研究概要	これまで、我々は低親和性FcεRII/CD23に対するモノクロ-ナル抗体作製及びcDNAクロ-ニングを行ってきたが、今回の申請期間中にはFcεRII/CD23がインタ-ロイキンのみならずPAFやTGFーβなどによっても様々に発現調節される事を示し、さらにこれまで決着のつかなかったヒトT細胞によるFcεRII/CD23産生を証明しその発現調節機構の一端を示すに至り(J.Immunol,in press)病的T細胞に発現するCD23研究に糸口を与えた。また、FcεRII/CD23がEBVによる細胞不死化にともなって強発現する事よりFcεRII/CD23を介したシグナル伝達機構に興味が持たれていたが、我々は、FCεRII/CD23cDNAを遺伝子導入したヒトNK様細胞株YTSERにおいてFcεRII/CD23がチロシン燐酸化酵素fynと会合している事を示し(Proc.Natl.Acad.Sci.in press)、さらに内因性にFcεRII/CD23を発現しているB細胞株においては、YTSERとは異なるチロシン燐酸化酵素が会合している事が明らかになり今後これら分子の解明を行いFcεRII/CD23を介したシグナル伝達機構の解析を進めて行きたい。また、抗CD23モノクロ-ナル抗体を用いたサンドイッチELISA法による血清中FcεRII/CD23測定のキット化にともないリュウマチやSLEなどの自己免疫疾患等で血清中低親和性FcεRII/CD23が増加する事が明かとなり、そのFcεRII/CD23の病態への関与の仕方を研究する目的で25Kd可溶型性FcεRII/CD23の大量生産を試み(ミドリ十字と共同)産生に成功しつつあり今後はこれらを用いてさらに低親和性FcεRII/CD23の生物学的役割について解析を進めたい。

研究成果
(13件)

すべてその他

すべて文献書誌 (13件)

[文献書誌] Katsuji Sugie: "FceRII/CD23ーMediated Activation Pathway Associated with a Protein Tyrosine Kinase." Proc.Natl.Acad.Sci.,U.S.A.
[文献書誌] Takumi Kawabe: "Induction of FcεRII/CD23 on PHA Activated Human Peripheral Blood T Lymphocytes: I.Enhancement by ILー2and ILー4." J.Immunol.
[文献書誌] Hamami Watanabe: "Transforming growth factor β and dexamethasone suppress the expression of Fcε receptor 2 (CD23) on a human eosinophilic cell line Eolー3." Immunol.Letters. 25. 313-318 (1990)
[文献書誌] Tomoaki Matsumoto: "Serum levels of soluble ILー2 receptor,ILー4 and IgEーbinding factors in childhood allergic diseases." Clin.Exp.Immunol.
[文献書誌] Junji Yodoi: "FcεRII/CD23:Regulation and Functional Roles in Cell Activation." Progress in Immunology VII.Proceeinngs of the 7th International Congress of Immunology Berlin 1989.VII. 724-731 (1989)
[文献書誌] Toshihide Shimada: "The Detection of IgA Fc Receptors (Fc αR) with Fluorescent Microspheres" J.Immunol.Method.
[文献書誌] MarieーClaude Denroy: "Interleukin 4 and interferons α and γ regulate Fcε R2/CD23mRNA expression on normal human B cells." Molecular Immunology. 27. 129-134 (1990)
[文献書誌] Takumi Kawabe: "Human FcεRII and IgEーbinding factor;triangular network with ILー2 and ILー4" 29th Forum in Immunology,Institut Pasteur/Elsevier. 141. 82-85 (1990)
[文献書誌] Junji Yodoi: "Function,processing and structure of Fc receptor" 29th Forum in Immunology,Institut Pasteur/Elsevier. 141. 59-108 (1990)
[文献書誌] Takumi Kawabe: "Role of PAF and Cytokines in the Modulation of FceRII/CD23 Expression on Human Eosinophiles" in Prostaglandins and Related Compounds II.Adv.in Prostaglandin,Thromboxane,and Leukotriene Research. 21.
[文献書誌] Vidar WendelーHansen: "Gene encoding CD23 leukocyte antigen (Fcεーreceptor 2) is located on human chromosome 19." Somatic Cell and Molecular Genetics. 16. 283-285 (1990)
[文献書誌] T.Matsumoto: "Decreased serum levels of IgE and IgEーbinding factors in individuals infected with human Tー lymphoblastoid virus type 1 (HTLVーI)" Clin.Exp.Immuol.81. 207-211 (1990)
[文献書誌] T.Matsumoto: "FcεRII on t cells and IgEーbinding factors in patients with atopic asthma." Pediatric allergy and Immunol.1. 21-25 (1990)

1990 年度 実績報告書

増殖因子受容体およびリンパ球活性化調節因子としてのヒトIgEーFcレセプタ-解析

研究代表者

淀井 淳司 京都大学, ウィルス研究所, 教授 (80108993)

研究成果

[文献書誌] Katsuji Sugie: "FceRII/CD23ーMediated Activation Pathway Associated with a Protein Tyrosine Kinase." Proc.Natl.Acad.Sci.,U.S.A.

[文献書誌] Takumi Kawabe: "Induction of FcεRII/CD23 on PHA Activated Human Peripheral Blood T Lymphocytes: I.Enhancement by ILー2and ILー4." J.Immunol.

[文献書誌] Hamami Watanabe: "Transforming growth factor β and dexamethasone suppress the expression of Fcε receptor 2 (CD23) on a human eosinophilic cell line Eolー3." Immunol.Letters. 25. 313-318 (1990)

[文献書誌] Tomoaki Matsumoto: "Serum levels of soluble ILー2 receptor,ILー4 and IgEーbinding factors in childhood allergic diseases." Clin.Exp.Immunol.

[文献書誌] Junji Yodoi: "FcεRII/CD23:Regulation and Functional Roles in Cell Activation." Progress in Immunology VII.Proceeinngs of the 7th International Congress of Immunology Berlin 1989.VII. 724-731 (1989)

[文献書誌] Toshihide Shimada: "The Detection of IgA Fc Receptors (Fc αR) with Fluorescent Microspheres" J.Immunol.Method.

[文献書誌] MarieーClaude Denroy: "Interleukin 4 and interferons α and γ regulate Fcε R2/CD23mRNA expression on normal human B cells." Molecular Immunology. 27. 129-134 (1990)

[文献書誌] Takumi Kawabe: "Human FcεRII and IgEーbinding factor;triangular network with ILー2 and ILー4" 29th Forum in Immunology,Institut Pasteur/Elsevier. 141. 82-85 (1990)

[文献書誌] Junji Yodoi: "Function,processing and structure of Fc receptor" 29th Forum in Immunology,Institut Pasteur/Elsevier. 141. 59-108 (1990)

[文献書誌] Takumi Kawabe: "Role of PAF and Cytokines in the Modulation of FceRII/CD23 Expression on Human Eosinophiles" in Prostaglandins and Related Compounds II.Adv.in Prostaglandin,Thromboxane,and Leukotriene Research. 21.

[文献書誌] Vidar WendelーHansen: "Gene encoding CD23 leukocyte antigen (Fcεーreceptor 2) is located on human chromosome 19." Somatic Cell and Molecular Genetics. 16. 283-285 (1990)

[文献書誌] T.Matsumoto: "Decreased serum levels of IgE and IgEーbinding factors in individuals infected with human Tー lymphoblastoid virus type 1 (HTLVーI)" Clin.Exp.Immuol.81. 207-211 (1990)

[文献書誌] T.Matsumoto: "FcεRII on t cells and IgEーbinding factors in patients with atopic asthma." Pediatric allergy and Immunol.1. 21-25 (1990)

1990 年度実績報告書

淀井淳司京都大学, ウィルス研究所, 教授 (80108993)