1992 年度実績報告書

筋形成の分子機構

研究課題

研究課題/領域番号	03304008
研究機関	千葉大学
研究代表者	大日方昂千葉大学, 理学部, 教授 (40012413)
研究分担者	真崎知生京都大学, 医学部, 教授 (60009991) 鍋島陽一国立精神神経センター, 神経研, 部長 (60108024) 佐藤矩行京都大学, 理学部, 助教授 (30025481) 浅島誠横浜市立大学, 文理学部, 教授 (00090564) 嶋田裕千葉大学, 医学部, 教授 (70009116)
キーワード	筋分化 / 骨格筋細胞 / 中胚葉誘導 / MyoD / 転写制御 / 筋原線継形成 / 筋蛋白質 / 細胞系譜
研究概要	筋形成について、中胚葉誘導、細胞分化誘導、脱分化、遺伝子転写制御、収縮構造の形態形成など種々のレベルでの分子機構を検討した。 1)浅島はツメガエルの細胞培養液からアクチビンA、AB、Bおよびフォリスタチンを単離・精製し、濃度依存的な中胚葉誘導作用の詳細を解明、さらに高濃度アクチビンによる腎臓や心臓の分化誘導もを見いだした。 2)筋細胞系列の決定と分化、脱分化について、佐藤は原索動物ホヤで自律的に筋細胞分化を導く因子を探索し、UV照射により筋分化能を失った卯細胞に筋分化能を付与できる卵断片を得た。遠藤は筋分化、脱分化を可逆的に起こせる筋細胞株(SV40Tを導入したC2細胞)を用い、分化と脱分化時における細胞周期誘導因子群の発現変化を明らかにした。小沢は鶏胚の体節筋芽細胞の肢芽筋形成領域への移動に際し、ガイドとしての側板側の重要性を見いだし、それに関わる因子を検索した。 4)筋蛋白質遺伝子とその転写制御について、鍋島はミオシン軽鎖遺伝子の転写制御を解析、エンハンサーに存在するE-ボックスはMyoD、Myogeninと選別的に反応すること、プロモーター領域でもMyoDとmyogeninが異なる作用を示すこと、さらに転写制御因子群の複合作用の重要性をを明らかにした。佐藤はホヤでclusterをなす5つのアクチン遺伝子の構造を解明、その内で筋特異的に発現されるHrMA4について転写制御域を明らかにした。 5)収縮構造の形態形成に関連して、眞崎は新たに見いだされた115-kDa-αアクチニンの構造の決定と組識および発生段階依存的な発現、細胞内局在を明らかにした。大日方はアクチン集合制御因子コフィリンの筋型アイソフォームのcDNAを分離、構造と発現を解明、また筋細胞への蛍光標識コフィリンの導入およびコフィリンcDNAのトランスフェクション法により筋細胞内でのコフィリンの作用のしかたを明らかにした。嶋田は培養筋細胞にセンス、アンチセンスcDNAの導入によりトロポニンC、Iの発現量を改変、そのときの形態形成を調べた。

研究成果

(19件)

すべてその他

すべて文献書誌 (19件)

[文献書誌] Imamura,Michihiro: "A novel nonmuscle-actinin: Purification and characterization of chicekn lung α-actinin." Journal of Biological Chemistry. 267. 25927-25933 (1992)
[文献書誌] Noguchi,Jiro: "Complete primary structure and tissue exprission of chicken pectoralis M-protein." Journal of Biological Chemistry. 267. 20302-20310 (1992)
[文献書誌] Endo,Takeshi: "SV40 large T inhibits myogenic differentiation partially through inducing c-jun." J.Biochem.112. 321-329 (1992)
[文献書誌] Fukami,K.: "Requirement of phosphatidylinositol 4,5bisphosphate for -actinin function." Nature. 359. 150-152 (1992)
[文献書誌] Endo,Takeshi: "Retinoblastoma gene product Rb accumulates during myogenic differentiation and is deinduced by the expression of SV40 large T antigen." J.Biochem.112. 427-430 (1992)
[文献書誌] Ono,Shoichi: "Colocalization of ADF and cofilin in intranuclea actin rods of cultured muscle cells." J.Muscle Res.Cell Motility. (1993)
[文献書誌] Makabe,K.W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells." Zool.Sci.9. 569-573 (1992)
[文献書誌] Hikosaka,A.: "Introduction and expression of recombinant genes in ascidian embryox." Develop.Growth Differ.34. 627-634 (1992)
[文献書誌] Hayashi,Kensuke: "Vimentin expression pattern is different between the flank region and limb regions of somatopleural mesoderm in the chicken embryo." Development Growth and Differentiation. 50. (1993)
[文献書誌] Komiyama,M.: "Spatial relationship of nebulin relative to other myofibrillar proteins during myogenesis in embryonic chick skeletal muscle cells in vitro." J.Muscle Res.Cell Motil.13. 48-54 (1992)
[文献書誌] Sonoda,M.: "Fine structure of transverse tubules and the sarcoplasmic reticulum at the myotendinous junctuo of stretced muscle fibers of the rat." Microsc.Res.Tech.24. 281-286 (1993)
[文献書誌] Sonoda,M.: "Internediate voltage electron microscopy of transverse tubules at myotendinous junctions." Microsc.Res.Tech.
[文献書誌] Abe,Hiroshi: "Cellular distribution and intranuclear localization of cofilin in cultured myotubes." Exp.Cell Res.(1993)
[文献書誌] Fujisawa-Sehara,A.: "Differential Trans-activation of muscle-specific regulatory elements including the MLC box by chicekn myod,myogenin,and MRF4." J.Biol.Chem.267. 10031-10038 (1992)
[文献書誌] Nishimura,S.: "A carboxyl-terminal truncated version of the activin receptor mediates activin signals in early Xenopus embryos." FEBS Ketters. 312. 169-173 (1992)
[文献書誌] Nakamura,Takanori: "Isolation and characterization of native activin B" J.Biol.Chem.267. 16385-16389 (1992)
[文献書誌] Uesuge,Yasuo: "Comparative study of sexual dimorphism of the innominate bone in rodents and amphibians." Anatomical Record. 234. 432-437 (1992)
[文献書誌] Kusakabe,T.: "Tunicate muscle actin genes:Structure and organization as a gene cluster." J.Mol.Biol.227. 900-955 (1992)
[文献書誌] Kouchi,K.: "Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes:an immunoelectron microscopic study." J.Muscle Res.Cell Motil.

1992 年度 実績報告書

筋形成の分子機構

研究代表者

大日方 昂 千葉大学, 理学部, 教授 (40012413)

研究成果

[文献書誌] Imamura,Michihiro: "A novel nonmuscle-actinin: Purification and characterization of chicekn lung α-actinin." Journal of Biological Chemistry. 267. 25927-25933 (1992)

[文献書誌] Noguchi,Jiro: "Complete primary structure and tissue exprission of chicken pectoralis M-protein." Journal of Biological Chemistry. 267. 20302-20310 (1992)

[文献書誌] Endo,Takeshi: "SV40 large T inhibits myogenic differentiation partially through inducing c-jun." J.Biochem.112. 321-329 (1992)

[文献書誌] Fukami,K.: "Requirement of phosphatidylinositol 4,5bisphosphate for -actinin function." Nature. 359. 150-152 (1992)

[文献書誌] Endo,Takeshi: "Retinoblastoma gene product Rb accumulates during myogenic differentiation and is deinduced by the expression of SV40 large T antigen." J.Biochem.112. 427-430 (1992)

[文献書誌] Ono,Shoichi: "Colocalization of ADF and cofilin in intranuclea actin rods of cultured muscle cells." J.Muscle Res.Cell Motility. (1993)

[文献書誌] Makabe,K.W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells." Zool.Sci.9. 569-573 (1992)

[文献書誌] Hikosaka,A.: "Introduction and expression of recombinant genes in ascidian embryox." Develop.Growth Differ.34. 627-634 (1992)

[文献書誌] Hayashi,Kensuke: "Vimentin expression pattern is different between the flank region and limb regions of somatopleural mesoderm in the chicken embryo." Development Growth and Differentiation. 50. (1993)

[文献書誌] Komiyama,M.: "Spatial relationship of nebulin relative to other myofibrillar proteins during myogenesis in embryonic chick skeletal muscle cells in vitro." J.Muscle Res.Cell Motil.13. 48-54 (1992)

[文献書誌] Sonoda,M.: "Fine structure of transverse tubules and the sarcoplasmic reticulum at the myotendinous junctuo of stretced muscle fibers of the rat." Microsc.Res.Tech.24. 281-286 (1993)

[文献書誌] Sonoda,M.: "Internediate voltage electron microscopy of transverse tubules at myotendinous junctions." Microsc.Res.Tech.

[文献書誌] Abe,Hiroshi: "Cellular distribution and intranuclear localization of cofilin in cultured myotubes." Exp.Cell Res.(1993)

[文献書誌] Fujisawa-Sehara,A.: "Differential Trans-activation of muscle-specific regulatory elements including the MLC box by chicekn myod,myogenin,and MRF4." J.Biol.Chem.267. 10031-10038 (1992)

[文献書誌] Nishimura,S.: "A carboxyl-terminal truncated version of the activin receptor mediates activin signals in early Xenopus embryos." FEBS Ketters. 312. 169-173 (1992)

[文献書誌] Nakamura,Takanori: "Isolation and characterization of native activin B" J.Biol.Chem.267. 16385-16389 (1992)

[文献書誌] Uesuge,Yasuo: "Comparative study of sexual dimorphism of the innominate bone in rodents and amphibians." Anatomical Record. 234. 432-437 (1992)

[文献書誌] Kusakabe,T.: "Tunicate muscle actin genes:Structure and organization as a gene cluster." J.Mol.Biol.227. 900-955 (1992)

[文献書誌] Kouchi,K.: "Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes:an immunoelectron microscopic study." J.Muscle Res.Cell Motil.

1992 年度実績報告書

大日方昂千葉大学, 理学部, 教授 (40012413)