1993 年度実績報告書

ヒトヘルペスウイルス群による感染発症機構の分子生物学的解析

研究課題

研究課題/領域番号	03304031
研究機関	岡山大学
研究代表者	新居志郎岡山大学, 医学部, 教授 (40029757)
研究分担者	山西弘一大阪大学, 微生物病研究所, 教授 (10029811) 南嶋洋一宮崎医科大学, 教授 (80041284) 大里外誉郎北海道大学, 医学部・附属癌研究施設, 教授 (30000912) 西山幸広名古屋大学, 医学部・附属病態制御研究施設, 助教授 (60115615) 森良一九州大学, 医学部, 教授 (50038692)
キーワード	ヘルペスウイルス / 単純ヘルペスウイルス / EBウイルス / サイトメガロウイルス / ヒトヘルペスウイルス6 / 病原性 / ヘルペスウイルス感染症 / 分子生物学
研究概要	1.単純ヘルペスウイルス(HSV)の感染発症機構: HSVの変異株の選択には、ウイルス側および細胞側の因子が関与していることを明らかにした(新居)。HSV糖蛋白gCのマウス網膜病原性への関与を検討した(森)。酸化電位水の殺ヘルペスウイルス作用を明らかにした(清水)。US3プロテインキナーゼの標的蛋白は、US9にコードされるテグメント蛋白であることが示唆された(西山)。 2.EBウイルス(EBV)の感染発症機構: EBVが、原発性胃癌の1割と原因的に密接に関連することを明らかにした(大里)。潜伏状態からウイルス複製状態へ移行するスイッチであるBZLF遺伝子産物は、他の遺伝子のプロモーター中に存在するエンハンサーに作用し転写を活性化することを明らかにした(高田)。潜在EBVの存在様式とその遺伝子発現機構を解析した(平井)。 3.サイトメガロウイルス(CMV)の感染発症機構: ヒト子宮組織にCMVを検出した(古川)。易感染性宿主のCMV感染症の発症予知因子として、CMV抗原血症およびDNA血症の意義を明かにした(南嶋)。CMVの初期遺伝子産物の解析を行った(平井)。網膜色素細胞由来培養細胞におけるCMVの潜伏感染系の解析を行った(倉田)。 4.ヒトヘルペスウイルス6および7(HHV-6,HHV-7)の感染発症機構: ホジキンリンパ腫のリンパ節にHHV-6抗原を認めた症例を報告した(倉田)。HHV-6の前初期遺伝子の解析を行った(山西)。健常人唾液から高率にHHV-7が分離されることを示した(森)。SUP-T1細胞がHHV-7の好適な宿主細胞であることを示した(新居)。

研究成果
(41件)

すべてその他

すべて文献書誌 (41件)

[文献書誌] Tateishi,K.: "Detection of herpes simplex virus in saliva from 1,000 oral surgeryoutpatients by the polymerase chain reaction(PCR)and virus isolation." J.Oral Pathol.Med.〈印刷中〉. (1994)
[文献書誌] Shimizu.Y: "Virucidal and bactricidal effect of electrolyzed oxidizing water." J.Dentl.Res.〈印刷中〉. (1994)
[文献書誌] Katsumoto.T: "A Device for in situ Embedding of Monolayer Cultures in Lowicryl K4M." J.Electron Microsc.42. 205-207 (1993)
[文献書誌] Sakaoka,H.: "Quantitative analysis of genomic polymorphism of herpes simplex virus type 1 strains from six countries:studies of molecular evolution and molecular epidemiology of the virus." J.Gen.Virol.〈印刷中〉. (1994)
[文献書誌] Yamashita,Y: "Down-regulation of the surface expression of class I MHC antigens by human cytomegalovirus." Virology. 193. 727-736 (1993)
[文献書誌] Nishiyama,Y.: "The US9,10,11,and 12 genes of herpes simplex virus type 1 are of no importance for its neurovirulence and latency in mice." Virology. 194. 419-423 (1993)
[文献書誌] Tsurumi,T.: "Functional expression and characterization of the Epstein-Barr virus DNA polymerase catalytic subunit." J.Virol.67. 4651-4658 (1993)
[文献書誌] Masuda,A.: "Synthesis and antiviral activity of adeonsine deaminase-resistant oxecetanocin A derivatives,2-halogeno-oxetanocin A." J.Antibiotics. 46. 1034-1037 (1993)
[文献書誌] Daikoku,T.: "Purification and biochemical characterization of the protein kinase encoded by the US3 gene of herpes simplex virus type 2." Virology. 197. 685-694 (1993)
[文献書誌] Tsurumi,T.: "Functional interaction between Epstein Barr Virus DNA polymerase catalytic subunit and its accessary subunit in vitro." J.Virol.67. 7648-7653 (1993)
[文献書誌] Kurachi,R.: "The pathogenicity of a US3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice." Arch.Virol.133. 259-273 (1993)
[文献書誌] Okano,M.: "High irradiation sensitivity of Epstein-Barr virus(EBV)in lymphoblastoid cells from patients with ataxia telangiectasia and EBV genome-positive Burkitt's lymphoma." Int.J.Oncol.2. 1023-1026 (1993)
[文献書誌] Okano,M.: "Epstein-Barr virus hypersensitivity of lymphocytes from patients with ataxia telangiectasia." Int.J.Oncol.2. 1027-1031 (1993)
[文献書誌] Tokunaga,M.: "Epstein-Barr virus in adult T-cell leukemia/lymphoma." Am.J.Pathol.143. 1263-1269 (1993)
[文献書誌] Adler,S.P.: "Antibodies induced by a primary cytomegalovirus infection react with human herpesvirus 6 proteins." J.Infect.Dis.168. 1119-1126 (1993)
[文献書誌] Kumura,E.: "Human herpesvirus 6 and intussusception." Pediatric Infect.Dis.J.12. 788-789 (1993)
[文献書誌] Imai,S.: "Epstein-Barr virus genomic sequences and specific antibodies in cerebrospinal fluid in Children with neurologic complications of acute and reactivated EBV infections." J.Med.Virol.40. 278-284 (1993)
[文献書誌] Tachi,N.: "Detection of Epstein-Barr virus genome in cerebrospinal fluid from a patient with acquired chorea by the polymerase chain reaction." J.Child Neurol.8. 424-425 (1993)
[文献書誌] Sasajima,Y.: "High expression of the Epstein-Barr virus latent protein EBNA2 on pyothorax-associated lymphomas(PALs)." Amer.J.Pathol.143. 1280-1285 (1993)
[文献書誌] Goto,T.: "Detection of virus-related genes in virus-infected cells by in situ hybridization." J.Clin.Electron Microsc.25. 5-6 (1993)
[文献書誌] Shibata,M.: "Detection of human cytomegalovirus DNA in dried newborn blood filter paper." J.Virol.Methods.〈印刷中〉.
[文献書誌] Kikuta,H.: "Fatal Epstein-Barr virus-associated hemophagocytic syndrome." Blood. 〈印刷中〉.
[文献書誌] Nishiyama,K.: "The pathogenicity of a US3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice." Arch.Virol.〈印刷中〉.
[文献書誌] Kondo,K.: "Association of human herpesvirus 6 infection of the central nervous system with recurrence of febrile convulsion." J.Infect.Dis.167. 1197-1200 (1993)
[文献書誌] Liu,Y.: "Induction of bilateral retinal necrosis in mice by unilateral intracameral inoculation of a glycoprotein-C deficient clinical isolate of herpes simplex virus type 1." Arch.Virol.129. 105-118 (1993)
[文献書誌] Toh,Y.: "Molecular characterization of naturally occurring glycoprotein C-negative herpes simplex virus type 1." Arch.Virol.129. 119-130 (1993)
[文献書誌] Toh,Y.: "Nucleotide sequence of the major DNA-binding protein gene of herpes simplex virus type 2 and a comparison with type 1 counterpart." Arch.Virol.129. 183-196 (1993)
[文献書誌] Tanaka,S.: "Molecular analysis of a neurovirulent herpes simplex virus type 2 strain with reduced thymidine kinase activity." Arch.Virol.131. 61-73 (1993)
[文献書誌] Liu,Y.: "Necrotizing chorioretinitis in mice inoculated with herpes simplex virus type 1 with or without glycoprotein C:Anterior chamber-associated immune deviation does not persist." Arch.Virol.132. 225-236 (1993)
[文献書誌] Hidaka,Y.: "Frequent isolation of human herpesvirus 7 from saliva samples." J.Med.Virol.40. 343-346 (1993)
[文献書誌] Shimizu,N.: "Analysis of the BZLF1 promoter of Epstein-Barr virus:Identification of an anti-immunoglobulin response sequence." J.Virol.67. 3240-3245 (1993)
[文献書誌] Chowdhury,M.I.H.,: "cAMP stimulates human immunodeficiency virus(HIV-1)from latently infected cells of monocyte-macrophage lineage:Synergism with TNF-α." Virology. 197. 345-349 (1993)
[文献書誌] Furukawa,T.: "Detection of human cytomegalovirus genome in uterus tissue." Arch.Virol.〈印刷中〉. (1994)
[文献書誌] Okano,M: "High Epstein-Barr virus(EBV)susceptibility of both lymphoblastoid and lymphoma cell lines derived from a Japanese patient with EBV genome-positive Burkitt's lymphoma." Tohoku J.Exp.Med.170. 71-79 (1993)
[文献書誌] Okano,M.: "Serologically activated Epstein-Barr virus infection in Japanese recipients with renal allografts." Immunol.Inf.Dis.3. 263-265 (1993)
[文献書誌] Ayata,M.: "Different antibody response to a neutralizing epitope of human cytomegalovirus glycoprotein B among seropositive individuals." J.Med.Virol.〈印刷中〉. (1994)
[文献書誌] Ibusuki K.: "Pathogenicity of murine cytomegalovirus for newborn mice:analysis with the attenuated mutants." Arch.Virol.131. 127-139 (1993)
[文献書誌] Akashi K.: "Severe infectious mononucleosis-like syndrome and primary human herpesvirus 6 infection in an adult." New Engl.J.Med.329. 168-171 (1993)
[文献書誌] Linhares M.I.S: "Cytomegalovirus,human herpesvirus 6 and human T cell leukemia virus infection in renal transplanted patients in the Northeast of Brazil." Brazilian J.Med.Biol.Res.26. 735-739 (1993)
[文献書誌] Koeda T.: "Progressive encephalopathy associated with cytomegalovirus infection without immune deficiency." J.Child Neurol.8. 373-377 (1993)
[文献書誌] Hamasuna R.: "Protective effect of carrageenan against murine cytomegalovirus infection in mice." Antivir.Chem.Chemother.4. 353-360 (1993)

1993 年度 実績報告書

ヒトヘルペスウイルス群による感染発症機構の分子生物学的解析

研究代表者

新居 志郎 岡山大学, 医学部, 教授 (40029757)

研究成果

[文献書誌] Tateishi,K.: "Detection of herpes simplex virus in saliva from 1,000 oral surgeryoutpatients by the polymerase chain reaction(PCR)and virus isolation." J.Oral Pathol.Med.〈印刷中〉. (1994)

[文献書誌] Shimizu.Y: "Virucidal and bactricidal effect of electrolyzed oxidizing water." J.Dentl.Res.〈印刷中〉. (1994)

[文献書誌] Katsumoto.T: "A Device for in situ Embedding of Monolayer Cultures in Lowicryl K4M." J.Electron Microsc.42. 205-207 (1993)

[文献書誌] Sakaoka,H.: "Quantitative analysis of genomic polymorphism of herpes simplex virus type 1 strains from six countries:studies of molecular evolution and molecular epidemiology of the virus." J.Gen.Virol.〈印刷中〉. (1994)

[文献書誌] Yamashita,Y: "Down-regulation of the surface expression of class I MHC antigens by human cytomegalovirus." Virology. 193. 727-736 (1993)

[文献書誌] Nishiyama,Y.: "The US9,10,11,and 12 genes of herpes simplex virus type 1 are of no importance for its neurovirulence and latency in mice." Virology. 194. 419-423 (1993)

[文献書誌] Tsurumi,T.: "Functional expression and characterization of the Epstein-Barr virus DNA polymerase catalytic subunit." J.Virol.67. 4651-4658 (1993)

[文献書誌] Masuda,A.: "Synthesis and antiviral activity of adeonsine deaminase-resistant oxecetanocin A derivatives,2-halogeno-oxetanocin A." J.Antibiotics. 46. 1034-1037 (1993)

[文献書誌] Daikoku,T.: "Purification and biochemical characterization of the protein kinase encoded by the US3 gene of herpes simplex virus type 2." Virology. 197. 685-694 (1993)

[文献書誌] Tsurumi,T.: "Functional interaction between Epstein Barr Virus DNA polymerase catalytic subunit and its accessary subunit in vitro." J.Virol.67. 7648-7653 (1993)

[文献書誌] Kurachi,R.: "The pathogenicity of a US3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice." Arch.Virol.133. 259-273 (1993)

[文献書誌] Okano,M.: "High irradiation sensitivity of Epstein-Barr virus(EBV)in lymphoblastoid cells from patients with ataxia telangiectasia and EBV genome-positive Burkitt's lymphoma." Int.J.Oncol.2. 1023-1026 (1993)

[文献書誌] Okano,M.: "Epstein-Barr virus hypersensitivity of lymphocytes from patients with ataxia telangiectasia." Int.J.Oncol.2. 1027-1031 (1993)

[文献書誌] Tokunaga,M.: "Epstein-Barr virus in adult T-cell leukemia/lymphoma." Am.J.Pathol.143. 1263-1269 (1993)

[文献書誌] Adler,S.P.: "Antibodies induced by a primary cytomegalovirus infection react with human herpesvirus 6 proteins." J.Infect.Dis.168. 1119-1126 (1993)

[文献書誌] Kumura,E.: "Human herpesvirus 6 and intussusception." Pediatric Infect.Dis.J.12. 788-789 (1993)

[文献書誌] Imai,S.: "Epstein-Barr virus genomic sequences and specific antibodies in cerebrospinal fluid in Children with neurologic complications of acute and reactivated EBV infections." J.Med.Virol.40. 278-284 (1993)

[文献書誌] Tachi,N.: "Detection of Epstein-Barr virus genome in cerebrospinal fluid from a patient with acquired chorea by the polymerase chain reaction." J.Child Neurol.8. 424-425 (1993)

[文献書誌] Sasajima,Y.: "High expression of the Epstein-Barr virus latent protein EBNA2 on pyothorax-associated lymphomas(PALs)." Amer.J.Pathol.143. 1280-1285 (1993)

[文献書誌] Goto,T.: "Detection of virus-related genes in virus-infected cells by in situ hybridization." J.Clin.Electron Microsc.25. 5-6 (1993)

[文献書誌] Shibata,M.: "Detection of human cytomegalovirus DNA in dried newborn blood filter paper." J.Virol.Methods.〈印刷中〉.

[文献書誌] Kikuta,H.: "Fatal Epstein-Barr virus-associated hemophagocytic syndrome." Blood. 〈印刷中〉.

[文献書誌] Nishiyama,K.: "The pathogenicity of a US3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice." Arch.Virol.〈印刷中〉.

[文献書誌] Kondo,K.: "Association of human herpesvirus 6 infection of the central nervous system with recurrence of febrile convulsion." J.Infect.Dis.167. 1197-1200 (1993)

[文献書誌] Liu,Y.: "Induction of bilateral retinal necrosis in mice by unilateral intracameral inoculation of a glycoprotein-C deficient clinical isolate of herpes simplex virus type 1." Arch.Virol.129. 105-118 (1993)

[文献書誌] Toh,Y.: "Molecular characterization of naturally occurring glycoprotein C-negative herpes simplex virus type 1." Arch.Virol.129. 119-130 (1993)

[文献書誌] Toh,Y.: "Nucleotide sequence of the major DNA-binding protein gene of herpes simplex virus type 2 and a comparison with type 1 counterpart." Arch.Virol.129. 183-196 (1993)

[文献書誌] Tanaka,S.: "Molecular analysis of a neurovirulent herpes simplex virus type 2 strain with reduced thymidine kinase activity." Arch.Virol.131. 61-73 (1993)

[文献書誌] Liu,Y.: "Necrotizing chorioretinitis in mice inoculated with herpes simplex virus type 1 with or without glycoprotein C:Anterior chamber-associated immune deviation does not persist." Arch.Virol.132. 225-236 (1993)

[文献書誌] Hidaka,Y.: "Frequent isolation of human herpesvirus 7 from saliva samples." J.Med.Virol.40. 343-346 (1993)

[文献書誌] Shimizu,N.: "Analysis of the BZLF1 promoter of Epstein-Barr virus:Identification of an anti-immunoglobulin response sequence." J.Virol.67. 3240-3245 (1993)

[文献書誌] Chowdhury,M.I.H.,: "cAMP stimulates human immunodeficiency virus(HIV-1)from latently infected cells of monocyte-macrophage lineage:Synergism with TNF-α." Virology. 197. 345-349 (1993)

[文献書誌] Furukawa,T.: "Detection of human cytomegalovirus genome in uterus tissue." Arch.Virol.〈印刷中〉. (1994)

[文献書誌] Okano,M: "High Epstein-Barr virus(EBV)susceptibility of both lymphoblastoid and lymphoma cell lines derived from a Japanese patient with EBV genome-positive Burkitt's lymphoma." Tohoku J.Exp.Med.170. 71-79 (1993)

[文献書誌] Okano,M.: "Serologically activated Epstein-Barr virus infection in Japanese recipients with renal allografts." Immunol.Inf.Dis.3. 263-265 (1993)

[文献書誌] Ayata,M.: "Different antibody response to a neutralizing epitope of human cytomegalovirus glycoprotein B among seropositive individuals." J.Med.Virol.〈印刷中〉. (1994)

[文献書誌] Ibusuki K.: "Pathogenicity of murine cytomegalovirus for newborn mice:analysis with the attenuated mutants." Arch.Virol.131. 127-139 (1993)

[文献書誌] Akashi K.: "Severe infectious mononucleosis-like syndrome and primary human herpesvirus 6 infection in an adult." New Engl.J.Med.329. 168-171 (1993)

[文献書誌] Linhares M.I.S: "Cytomegalovirus,human herpesvirus 6 and human T cell leukemia virus infection in renal transplanted patients in the Northeast of Brazil." Brazilian J.Med.Biol.Res.26. 735-739 (1993)

[文献書誌] Koeda T.: "Progressive encephalopathy associated with cytomegalovirus infection without immune deficiency." J.Child Neurol.8. 373-377 (1993)

[文献書誌] Hamasuna R.: "Protective effect of carrageenan against murine cytomegalovirus infection in mice." Antivir.Chem.Chemother.4. 353-360 (1993)

1993 年度実績報告書

新居志郎岡山大学, 医学部, 教授 (40029757)