1994 年度実績報告書

ゲノムの複製終結と分配制御

研究課題

研究課題/領域番号	04256103
研究機関	熊本大学
研究代表者	平賀壮太熊本大学, 医学部, 教授 (40027321)
研究分担者	小椋光熊本大学, 医学部, 助教授 (00158825) 西村昭子国立遺伝学研究所, 助教授 (20142002) 加藤潤一東京大学, 医科学研究所, 助手 (10194820) 永井和夫東京工業大学, 生命理工学部, 教授 (00011974) 岡崎恒子名古屋大学, 理学部, 教授 (10022584)
キーワード	MukB蛋白 / トポイソメラーゼ / FtsH蛋白 / ヒト染色体 / セントロメア / アルフォイドDNA / ミニ染色体 / 熱ショック転写因子
研究概要	1.大腸菌染色体分配に関与するmukB遺伝子の上流に存在するofr50の破壊株を解析して、この遺伝子(mukFと命名)もmkB遺伝子と同じく染色体分配に関与することを明らかにした(平賀)。 2.ヒト染色体セントロメア領域に存在するアルフォイドDNA中のCEN-B-BoxとCEN-B蛋白の複合体の高次構造について解析した(岡崎)。 3.トポイソメラーゼIIαとIIβの両方の活性阻害をする抗体を注入すると細胞は中記まで進がそれ以後は進まないことを明らかにした(菊池)。 4.大腸菌のトポイソメラーゼIV(parCとparE)と相互作用をする蛋白質を検索することを目標として、dominant negativeなparC変異を抑制する多重コピー抑制遺伝子を多数分離し、解析した(加藤)。 5.FtsH蛋白は熱ショック転写因子δ32を分解するATP、Zn++依存性の新しいタイプの蛋白分解酵素であることを明らかにした(小椋)。 6.DNA合成阻害時においても細胞分裂の阻害が起こりにくいcfcA、cfcB変異がそれぞれglyS、apaHの変異であることを明らかにし、細胞内のAppppAの量が細胞分裂の頻度を調節していることを明らかにした(西村昭子)。 7.Fプラスミドの分配遺伝子sopBの欠失が宿主細胞の増殖を阻害する現象を解析して、この増殖阻害現象はsopB欠失のミニFプラスミドでは見られないが、このミニFプラスミドに30〜40kb以上の長いDNA断片を挿入すると強く表れることを明らかにした(三木)。

研究成果

(29件)

すべてその他

すべて文献書誌 (29件)

[文献書誌] Adachi N.: "Mutant isolation of mouse DNA topoisomerase lla in yeast." Nucl.Acids.Res.22. 187-188 (1994)
[文献書誌] Kimura K.: "Identification of the nature of modification that causes the shift of DNA topoisomerase IIb to apparent higher molecular weight forms in the M phase." J.Biol.Chem.269. 24523-24526 (1994)
[文献書誌] Ohkata K.: "Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubles and is a potential regulator of M-phase microtubule dynamics." J.Cell Biol.(in press). (1995)
[文献書誌] Ohkubo Y.: "SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing cdk2,cdc2,and their partner cyclins." Exp.Cell Res.(in press). (1994)
[文献書誌] Ohsumi K.: "Meiosis-specific cell cycle regulation in maturing Xenopus oocytes." J.Cell Sci.(in press). (1994)
[文献書誌] Ohmori H.: "The pcsA gene is identical to dinD in Escherichia coli." J.Bacteriol.177. 156-165 (1995)
[文献書誌] Norris V.: "Cell cycle control:Prokaryotic solutions to eukaryotic problem?" J.theor.Biol.168. 227-230 (1994)
[文献書誌] Okada Y.: "Cytoplasmic axial filaments in Escherichia coli cells:possible function in the mechanism of chromosome segregation and cell division." J.Bacteriol.176. 917-922 (1994)
[文献書誌] Asai K.: "CThe identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase." Biopys.Biochem.Res.Commun.202. 340-345 (1994)
[文献書誌] Horiuchi T.: "Recombinational rescue of the stalled DNA replication fork;a model based on analysis of an E.coli strain with a chromosmal region difficult to replicate." J.Bacteriol.177. 783-791 (1995)
[文献書誌] Horiuchi T.: "A new type of E.coli recombinational hotspot which requires for activity both DNA replication termination events and the Chi sequence." Advance in Biophysics. (in press). (1995)
[文献書誌] Horiuchi T.: "The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA." J.Bacteriol.176. 4656-4663 (1994)
[文献書誌] Seki,N.: "Mapping of the human centromere protein B gene (CENPB) to chromosome 20p13 by fluorescence in situ hybridization." Genomics. 24. 187-188 (1994)
[文献書誌] Tatebayashi K.: "Structural analyses of DNA fragments integrated by illegitimate recombination in Schizosaccharomyces pombe." Mol.Gen.Genet.244. 111-119 (1994)
[文献書誌] 加藤潤一: "大腸菌とλファージの実験法" 細胞工学. 13. 753-756 (1994)
[文献書誌] Hoshino K.: "Inhibition of quinolones against topoisomerase IV from Escherichia coli and comparison with the inhibitory potency against DNA gyrase." Antimicrob.Agents.Chemother.(in press). (1994)
[文献書誌] Nagata,K.: "Set,aputative oncogene associated with leukemogenesis os required for adenovirus DNA replication." Proc.Natl.Sci.USA. (in press). (1995)
[文献書誌] Kikuchi Y.: "A new yeast gene,HTR1,required for high tempereture is needed for recovery from mating pheromone-induced G1 arrest." Hum Mol Genet. 245. 107-116 (1994)
[文献書誌] Yamanaka K.: "Multicopy suppressors,mssA and mssB,of an smbA mutation of Escherichia coli." Mol.Gen.Genet.243. 9-16 (1994)
[文献書誌] Yamanaka K.: "Characterization of translucent segments observed in an smbA mutant of Escherichia coli." FEMS Microbiol.Lett.166. 61-66 (1994)
[文献書誌] YSamanaka K.: "Two mutant alleles of mukB,a gene essential for chromosome partition in Escherichia coli." FEMS Microbiol.Lett.123. 27-32 (1994)
[文献書誌] Yamanaka K.: "Cloning,sequencing,and characterization of multicopy suppressors of a mukB mutation in Escherichia coli." Mol.Microbiol.13. 301-312 (1994)
[文献書誌] Ogawa T.: "Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli." J.Bacteriol.176. 1609-1615 (1994)
[文献書誌] Ikeno,M.: "Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range α-satellite DNA arrays of human chromosome 21." Hum Mol Genet. 3. 1245-1257 (1994)
[文献書誌] Miki T.: "Temperature shift-up leads to simultaneous and continuous plamid DNA relaxation and induction of DnaK and GroES proteins in anaerobically growing Escherichia coli cells." FEMS Microbiol.Lett.121. 333-336 (1994)
[文献書誌] Miki T.: "Identification of DNA topoisomerases involved in immediate and transient DNA relazation induced by heat shock in Escherichia coli." Mol.Gen.Genet.244. 451-455 (1994)
[文献書誌] Miki T.: "Loss of flageration in dnaA mutants of Escherichia coli." J.Bacteriol.176. 5544-5546 (1994)
[文献書誌] Miki T.: "Lysozyme requires fluctuation of the active site for the manifestation of activity." Protein Engineering. 7. 743-748 (1994)
[文献書誌] Miki T.: "Formation of a-helix 88-98 is essential in the establishment of higher-order structure from reduced lysozyme." J.Mol.Biol.235. 1312-1317 (1994)

1994 年度 実績報告書

ゲノムの複製終結と分配制御

研究代表者

平賀 壮太 熊本大学, 医学部, 教授 (40027321)

研究成果

[文献書誌] Adachi N.: "Mutant isolation of mouse DNA topoisomerase lla in yeast." Nucl.Acids.Res.22. 187-188 (1994)

[文献書誌] Kimura K.: "Identification of the nature of modification that causes the shift of DNA topoisomerase IIb to apparent higher molecular weight forms in the M phase." J.Biol.Chem.269. 24523-24526 (1994)

[文献書誌] Ohkata K.: "Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubles and is a potential regulator of M-phase microtubule dynamics." J.Cell Biol.(in press). (1995)

[文献書誌] Ohkubo Y.: "SV40 large T antigen reinduces the cell cycle in terminally differentiated myotubes through inducing cdk2,cdc2,and their partner cyclins." Exp.Cell Res.(in press). (1994)

[文献書誌] Ohsumi K.: "Meiosis-specific cell cycle regulation in maturing Xenopus oocytes." J.Cell Sci.(in press). (1994)

[文献書誌] Ohmori H.: "The pcsA gene is identical to dinD in Escherichia coli." J.Bacteriol.177. 156-165 (1995)

[文献書誌] Norris V.: "Cell cycle control:Prokaryotic solutions to eukaryotic problem?" J.theor.Biol.168. 227-230 (1994)

[文献書誌] Okada Y.: "Cytoplasmic axial filaments in Escherichia coli cells:possible function in the mechanism of chromosome segregation and cell division." J.Bacteriol.176. 917-922 (1994)

[文献書誌] Asai K.: "CThe identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase." Biopys.Biochem.Res.Commun.202. 340-345 (1994)

[文献書誌] Horiuchi T.: "Recombinational rescue of the stalled DNA replication fork;a model based on analysis of an E.coli strain with a chromosmal region difficult to replicate." J.Bacteriol.177. 783-791 (1995)

[文献書誌] Horiuchi T.: "A new type of E.coli recombinational hotspot which requires for activity both DNA replication termination events and the Chi sequence." Advance in Biophysics. (in press). (1995)

[文献書誌] Horiuchi T.: "The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA." J.Bacteriol.176. 4656-4663 (1994)

[文献書誌] Seki,N.: "Mapping of the human centromere protein B gene (CENPB) to chromosome 20p13 by fluorescence in situ hybridization." Genomics. 24. 187-188 (1994)

[文献書誌] Tatebayashi K.: "Structural analyses of DNA fragments integrated by illegitimate recombination in Schizosaccharomyces pombe." Mol.Gen.Genet.244. 111-119 (1994)

[文献書誌] 加藤潤一: "大腸菌とλファージの実験法" 細胞工学. 13. 753-756 (1994)

[文献書誌] Hoshino K.: "Inhibition of quinolones against topoisomerase IV from Escherichia coli and comparison with the inhibitory potency against DNA gyrase." Antimicrob.Agents.Chemother.(in press). (1994)

[文献書誌] Nagata,K.: "Set,aputative oncogene associated with leukemogenesis os required for adenovirus DNA replication." Proc.Natl.Sci.USA. (in press). (1995)

[文献書誌] Kikuchi Y.: "A new yeast gene,HTR1,required for high tempereture is needed for recovery from mating pheromone-induced G1 arrest." Hum Mol Genet. 245. 107-116 (1994)

[文献書誌] Yamanaka K.: "Multicopy suppressors,mssA and mssB,of an smbA mutation of Escherichia coli." Mol.Gen.Genet.243. 9-16 (1994)

[文献書誌] Yamanaka K.: "Characterization of translucent segments observed in an smbA mutant of Escherichia coli." FEMS Microbiol.Lett.166. 61-66 (1994)

[文献書誌] YSamanaka K.: "Two mutant alleles of mukB,a gene essential for chromosome partition in Escherichia coli." FEMS Microbiol.Lett.123. 27-32 (1994)

[文献書誌] Yamanaka K.: "Cloning,sequencing,and characterization of multicopy suppressors of a mukB mutation in Escherichia coli." Mol.Microbiol.13. 301-312 (1994)

[文献書誌] Ogawa T.: "Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli." J.Bacteriol.176. 1609-1615 (1994)

[文献書誌] Ikeno,M.: "Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range α-satellite DNA arrays of human chromosome 21." Hum Mol Genet. 3. 1245-1257 (1994)

[文献書誌] Miki T.: "Temperature shift-up leads to simultaneous and continuous plamid DNA relaxation and induction of DnaK and GroES proteins in anaerobically growing Escherichia coli cells." FEMS Microbiol.Lett.121. 333-336 (1994)

[文献書誌] Miki T.: "Identification of DNA topoisomerases involved in immediate and transient DNA relazation induced by heat shock in Escherichia coli." Mol.Gen.Genet.244. 451-455 (1994)

[文献書誌] Miki T.: "Loss of flageration in dnaA mutants of Escherichia coli." J.Bacteriol.176. 5544-5546 (1994)

[文献書誌] Miki T.: "Lysozyme requires fluctuation of the active site for the manifestation of activity." Protein Engineering. 7. 743-748 (1994)

[文献書誌] Miki T.: "Formation of a-helix 88-98 is essential in the establishment of higher-order structure from reduced lysozyme." J.Mol.Biol.235. 1312-1317 (1994)

1994 年度実績報告書

平賀壮太熊本大学, 医学部, 教授 (40027321)