1994 年度実績報告書

小胞体経路の蛋白質選別輸送の分子機構

研究課題

研究課題/領域番号	04259106
研究機関	九州大学
研究代表者	大村恒雄九州大学, 名誉教授 (80029933)
研究分担者	三原勝芳九州大学, 大学院・医学系研究科, 助教授 (40029963) 姫野勝九州大学, 薬学部, 教授 (50037602) 中野明彦東京大学, 大学院・理学系研究科, 助教授 (90142140) 勝沼信彦徳島文理大学, 健康科学研究所, 教授 (50035375) 池原征夫福岡大学, 医学部, 教授 (70037612)
キーワード	蛋白質選別輸送 / 小胞体 / ゴルジ装置 / 蛋白質膜透過 / 小胞輸送 / リソソーム
研究概要	真核細胞において粗面小胞体の膜結合リボソームで合成される種々の蛋白質が小胞体からゴルジ装置を経て細胞表層やリソソームに移行する輸送経路を研究対象とし、1)新生ペプチドの小胞体認識機構、2)ペプチドの小胞体膜透過機構、小胞体膜への組み込み機構、3)小胞体からゴルジ装体を経て細胞表層に至る蛋白質の小胞輸送機構、4)小胞輸送においてのゴルジ体の機能、5)小胞輸送過程においての蛋白質の修飾機構、6)リソソームへの蛋白質の選別輸送機構、7)エンドサイトーシス、エキソサイトーシスの分子機構、について分担して研究した。主要な研究実績は次の通りである。1)酵母シグナル認識粒子(SRP)のSRP受容体との相互作用とGTPase活性の制御機構を変異株を用い解析した(天谷)。2)N末にシグナルアンカー配列(SA)をもつP450の膜への組み込み機構を検討し、SAに続くプロリンに富む配列の重要性を確かめた(三原)。C末に膜挿入配列をもつアルデヒド脱水素酵素の小胞体への組み込みシグナルを解析した(山本)。3)小胞体-ゴルジ体間輸送に関与するSarlpの機能を解析しGTPの加水分解が輸送小胞のゴルジ体への移行に必須であることを確かめた(中野)。Rab3Aによる小胞輸送制御を解析し、Rab3Aの標的蛋白質Rabphilin-3Aの結合蛋白質としてGTPcyclohydrolase 1を同定した(佐々木)。4)ゴルジ膜の細胞質側に局在する分子量372kDa、170kDa,99kDaの3種類の蛋白質を同定し一次構造を決定した(池原)5)外来抗原のプロセシングをCathepsin Bが行っていることを証明した(勝沼)。6)リソソームの主要膜成分LGP96がエンドソーム-リソソーム間の融合に関与する証拠を得た(姫野)。自食過程へのユビキチン系の関与を免疫電顕法で確かめた(横田)。7)内皮細胞層でのAngiotensinのトランスサイトーシスへの液胞型H^+ ATPaseの関与を確認した(高野)。

研究成果
(23件)

すべてその他

すべて文献書誌 (23件)

[文献書誌] K.Oda: "Conversion of secretory proteins into membrane proteins by fusing with a glycosyl-phosphatidylinositol anchor signal of alkaline phosphatase." Biochem.J.301. 577-583 (1994)
[文献書誌] M.Sohda: "Molecular cloning and sequence analysis of a human 372-kDa protein localized in the Golgi complex." Biochem.Biophys.Res.Commun.205. 1399-1408 (1994)
[文献書誌] N.Katunuma: "Immunological significances of invariant chain from the aspect of its structural homology with cystatin family." FEBS-letter. 349. 265-269 (1994)
[文献書誌] A.Mizoguchi: "Localization of Rabphilin-3A on the synaptic vesicle." Biochem.Biophys.Res.Commun.202. 1235-1243 (1994)
[文献書誌] K.Imazumi: "Identification of a Rabphilin-3A interacting protein as GTP cyclohydorase I in PC12 cells." Biochem.Biophys.Res.Commun.205. 1409-1416 (1994)
[文献書誌] M.Kato: "Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II." Biochem.Biophys.Res.Commun.205. 1776-1784 (1994)
[文献書誌] A.Miyazaki: "Comparison of kinetic properties between MSS4 and Rab3A FRF GDP/GTP exchange proteins." FEBS Lett.350. 333-336 (1994)
[文献書誌] Y.Fujita: "GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein." FEBS Lett.353. 67-70 (1994)
[文献書誌] C.Mineo: "Angiotensin II binding activity in cultured porcine arterial endotehlial cells." Biochemical Pharmacology. 48. 1993-1995 (1994)
[文献書誌] T.Oka: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functionalintermediate of the ER-to-Golgi transport in yeast." J.Cell Biol.124. 425-434 (1994)
[文献書誌] K.Fujimura: "The Saccharomyces cerevisiae MSI4 gene encodes the yeast counterpart of componentA of Rab geranylgeranyltransferase." J.Biol.Chem.269. 9205-9212 (1994)
[文献書誌] A.Nakano: "Mutationalanalysis of the Sarl protein,a small GTPase which is essential for vesicular transport from the endoplasmic reticulum." J.Biochem.116. 243-247 (1994)
[文献書誌] S.Nishikawa: "Inhibition of endoplasmic reticulum(ER)-to-Golgi transport induces relocalization of binding protein(BiP) within the ER to form the BiP bodies." Mol.Biol.Cell.5. 1129-1143 (1994)
[文献書誌] K.Goto: "Localization of cathepsin B,D,and L in the rat osteoclast by immuno-light and -electron microscopy." Histochemistry. 101. 33-40 (1994)
[文献書誌] T.Kiyoshima: "Immunocytochemical localization of cathepsin L in the synovial lining calls of the rat temporomandibular joint." Archs oral Biol.39. 1049-1056 (1994)
[文献書誌] K.Murakami: "The transmembrane region of microsomal cytochrome p450 identified as the endoplasmic reticulum retention signal." J.Biochem.116. 164-175 (1994)
[文献書誌] N.Hachiya: "MSF,a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria." EMBO J.13. 5146-5154 (1994)
[文献書誌] R.Alam: "cDNA cloning and characterization of mitochondrial import stimulation factor(MSF) purified from rat liver cytosol." J.Biochem.116. 416-425 (1994)
[文献書誌] R.Masaki: "Microsomal aldehyde dehydrogenase is localized to the endoplasmic reticulum via its carboxyl-terminal 35 amino acid." J.Cell Biol.126. 1407-1420 (1994)
[文献書誌] K.Kambe: "Preferential localization of heat shock protein 47 in dilated endoplasmic reticulum of chicken chondrocytes." J.Histochem.Cytochem.42. 833-841 (1994)
[文献書誌] T.Michikawa: "Transmembrane topology and sites of N-glycosylation of inositol 1,4,5-trisphosphate receptor." J.Biol.Chem.269. 9184-9189 (1994)
[文献書誌] R-M.Hong: "Association of N-ethyleimide-sensitive factor with synaptic vesicles." FEBS Letters. 350. 253-257 (1994)
[文献書誌] S.Yokota: "Formation of autophagosomes during degradation of excess peroxisomes induced by di-(2-ethylhexyl)phthalate tretment.III.Fusion of early autophagosomes with lysosomal compartments." Eur.J.Cell Biol.66. 15-24 (1995)

1994 年度 実績報告書

小胞体経路の蛋白質選別輸送の分子機構

研究代表者

大村 恒雄 九州大学, 名誉教授 (80029933)

研究成果

[文献書誌] K.Oda: "Conversion of secretory proteins into membrane proteins by fusing with a glycosyl-phosphatidylinositol anchor signal of alkaline phosphatase." Biochem.J.301. 577-583 (1994)

[文献書誌] M.Sohda: "Molecular cloning and sequence analysis of a human 372-kDa protein localized in the Golgi complex." Biochem.Biophys.Res.Commun.205. 1399-1408 (1994)

[文献書誌] N.Katunuma: "Immunological significances of invariant chain from the aspect of its structural homology with cystatin family." FEBS-letter. 349. 265-269 (1994)

[文献書誌] A.Mizoguchi: "Localization of Rabphilin-3A on the synaptic vesicle." Biochem.Biophys.Res.Commun.202. 1235-1243 (1994)

[文献書誌] K.Imazumi: "Identification of a Rabphilin-3A interacting protein as GTP cyclohydorase I in PC12 cells." Biochem.Biophys.Res.Commun.205. 1409-1416 (1994)

[文献書誌] M.Kato: "Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinase II." Biochem.Biophys.Res.Commun.205. 1776-1784 (1994)

[文献書誌] A.Miyazaki: "Comparison of kinetic properties between MSS4 and Rab3A FRF GDP/GTP exchange proteins." FEBS Lett.350. 333-336 (1994)

[文献書誌] Y.Fujita: "GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein." FEBS Lett.353. 67-70 (1994)

[文献書誌] C.Mineo: "Angiotensin II binding activity in cultured porcine arterial endotehlial cells." Biochemical Pharmacology. 48. 1993-1995 (1994)

[文献書誌] T.Oka: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functionalintermediate of the ER-to-Golgi transport in yeast." J.Cell Biol.124. 425-434 (1994)

[文献書誌] K.Fujimura: "The Saccharomyces cerevisiae MSI4 gene encodes the yeast counterpart of componentA of Rab geranylgeranyltransferase." J.Biol.Chem.269. 9205-9212 (1994)

[文献書誌] A.Nakano: "Mutationalanalysis of the Sarl protein,a small GTPase which is essential for vesicular transport from the endoplasmic reticulum." J.Biochem.116. 243-247 (1994)

[文献書誌] S.Nishikawa: "Inhibition of endoplasmic reticulum(ER)-to-Golgi transport induces relocalization of binding protein(BiP) within the ER to form the BiP bodies." Mol.Biol.Cell.5. 1129-1143 (1994)

[文献書誌] K.Goto: "Localization of cathepsin B,D,and L in the rat osteoclast by immuno-light and -electron microscopy." Histochemistry. 101. 33-40 (1994)

[文献書誌] T.Kiyoshima: "Immunocytochemical localization of cathepsin L in the synovial lining calls of the rat temporomandibular joint." Archs oral Biol.39. 1049-1056 (1994)

[文献書誌] K.Murakami: "The transmembrane region of microsomal cytochrome p450 identified as the endoplasmic reticulum retention signal." J.Biochem.116. 164-175 (1994)

[文献書誌] N.Hachiya: "MSF,a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria." EMBO J.13. 5146-5154 (1994)

[文献書誌] R.Alam: "cDNA cloning and characterization of mitochondrial import stimulation factor(MSF) purified from rat liver cytosol." J.Biochem.116. 416-425 (1994)

[文献書誌] R.Masaki: "Microsomal aldehyde dehydrogenase is localized to the endoplasmic reticulum via its carboxyl-terminal 35 amino acid." J.Cell Biol.126. 1407-1420 (1994)

[文献書誌] K.Kambe: "Preferential localization of heat shock protein 47 in dilated endoplasmic reticulum of chicken chondrocytes." J.Histochem.Cytochem.42. 833-841 (1994)

[文献書誌] T.Michikawa: "Transmembrane topology and sites of N-glycosylation of inositol 1,4,5-trisphosphate receptor." J.Biol.Chem.269. 9184-9189 (1994)

[文献書誌] R-M.Hong: "Association of N-ethyleimide-sensitive factor with synaptic vesicles." FEBS Letters. 350. 253-257 (1994)

[文献書誌] S.Yokota: "Formation of autophagosomes during degradation of excess peroxisomes induced by di-(2-ethylhexyl)phthalate tretment.III.Fusion of early autophagosomes with lysosomal compartments." Eur.J.Cell Biol.66. 15-24 (1995)

1994 年度実績報告書

大村恒雄九州大学, 名誉教授 (80029933)