1993 年度実績報告書

光合成の環境適応の分子機構

研究課題

研究課題/領域番号	04273103
研究機関	岡崎国立共同研究機構
研究代表者	村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)
研究分担者	篠崎一雄理化学研究所, ライフサイエンス筑波研究センター, 主任研究員 (20124216) 藤伊正筑波大学, 生物科学系, 教授 (20011611) 杉山達夫名古屋大学, 農学部, 教授 (50023453) 水野猛名古屋大学, 農学部, 教授 (10174038) 和田敬四郎金沢大学, 理学部, 教授 (70028174)
キーワード	環境適応 / 低温 / 塩ストレス / 乾燥 / 無機イオン欠乏 / ラン藻
研究概要	研究目的:低温、塩、無イオン欠乏党の環境要因の変化への適応現象を分子レベルで解明すること、および適応能力のすぐれた形質転換植物を作成することを全体の目的とする。本年度は、環境適応に関与する遺伝子のクローニングを重要目標とし、さらにクローニングされた遺伝子の解析と、形質転換植物作成のための準備をおこなうことを目標とした。主要研究成果:(1)低温適応において重要な役割を担うDELTA12不飽和化酵素をE.coli中で大量発現させた。生じたDELTA12不飽和化酵素はフェレドキシンを電子受容体とすること、18:1(9)を18:1(9,12)に不飽和化するが18:1(11)は不飽和化しないことを明かにした。さらに部位特異的変異法により、鉄のリガンドを形成すると推定されるヒスチジン残基の内の5残基をアルギニンに変換した。この変換により不飽和化活性は消失した。(2)塩耐性に関与すると推定されるグリシンベタインを、E.coliのbet遺伝子オペロンをシアノバクテリアPCC7942に導入することによって細胞内に相当量を合成させた。またコムギのベタインアルデヒド脱水素酵素をタバコに導入して発現させ、外からベタインアルデヒドを添加することによってベタインを生合成させることができるようになった。(3)リン酸の欠乏を感知するセンサーおよびこれによってリン酸化を受ける転写制御因子のそれぞれの遺伝子を単離した。またこれらの因子によって誘導される2種類のタンパク質を同定したが、これらのタンパク質はリン酸の取り込み機構を形成していると推定された。

研究成果
(27件)

すべてその他

すべて文献書誌 (27件)

[文献書誌] Vigh,L.: "The primary signal in the biological perception of temperature:Pd‐catalyzed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803." Proc.Natl.Acad.Sci.U.S.A.90. 9090-9094 (1993)
[文献書誌] Wada,H.: "The contribution of membrane‐lipids to the ability of the photosynthetic machinery to tolerate temperature stress." Proc.Natl.Acad.Sci.U.S.A.in press. (1993)
[文献書誌] Mamedov,Mahir.: "Effects of glycinebetatine and unsaturation of membrane lipids on heat stability of photosynethetic electron‐transport and phosphorylation reactions in Synechocystis PCC6803." Biochem.Biophs.Actra. 1142. 1-5 (1993)
[文献書誌] Nishiyama,Y.: "Photosynthetic adaptation to high temperature associated with thylakoid membranes of Synechococcus PCC7002." Plant Cell Physiol.34. 337-343 (1993)
[文献書誌] Los,D.: "The temperature‐dependent expression of the desaturase gene desA in Synechocystis PCC803." FEBS Letters. 318. 57-60 (1993)
[文献書誌] Wada,H.: "The desA gene of the cyanobacterium Synechocystis sp.strain PCC6803 is the structural gene for DELTA12 desaturase." J.Bacteriol.175. 6056-6058 (1993)
[文献書誌] Sakamoto,T.: "Identification of conserved domains in the DELTA 12 desaturase of cyanobacteria." Plant Mol.Biol.in press. (1993)
[文献書誌] Morisaki,N.: "Effect of side‐chain structure on inhibition of yeast fatty‐acid synthase by cerulenin analogues." Eur.J.Biochem.211. 111-115 (1993)
[文献書誌] Ishitani,M.: "Regulation of glycinebetaine accumulation in the halotolerant cyanobacterium Aphanothece halophytica." Aust.J.Plant Physiol.20. 693-703 (1993)
[文献書誌] Ishitani,M.: "Betainealdehyde dehydrogenase in the gramineae:Levels in leaves of both betaine‐accumulating and nonaccumulating cereal plants ^1." Plant Cell Physiol. 34. 493-495 (1993)
[文献書誌] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of winter and spring bareley plants." Plant,Cell and Environment. 17. 89-95 (1993)
[文献書誌] Aiba,H.: "Sensor and regulator proteins from cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signaltransduction protein families:implication in the adaptive response to phosphate limitation." Mol.Microbiol.8. 81-91 (1993)
[文献書誌] Nagaya,M.: "Cloning of a sensory‐kinase‐encoding gene that belongs to the two‐component regulatory family from the cyanobacterium Synechococcus sp.PCC7942." Gene. 131. 119-124 (1993)
[文献書誌] Kanamura,K.: "The cyanobacterium,Synechococcus sp.PCC7942,possesses two distinct genes encoding cation‐transporting P‐type ATPases." FEBS Letters. 330. 99-104 (1993)
[文献書誌] Nagaya,M.: "A cyanobacterial gene encoding a protein with extensive homology to mammalian phosphoribosylpyrophosphate synthetase." Biosci.Biotech.Biochem.57. 1958-1959 (1993)
[文献書誌] Yamaguchi‐Shinozaki,K.: "Characterization of the expression of a desiccation‐responsive rD29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants." Mol.Gen.Genet.236. 331-340 (1993)
[文献書誌] Yamaguchi‐Shinozaki,K.: "Arabidopsis DNA encoding two desiccation‐responsive rd29 genes^<1.>" Plant Physiol.101. 1119‐1120 (1993)
[文献書誌] Kiyosue,T.: "Characterization of two cDNAs(ERD11 and ERD13)for dehydration‐inducible genes that encode putative glutathione S‐transferases in Arabidopsis thaliana L." FEBS Letters. 335. 189-192 (1993)
[文献書誌] Yamaguchi‐Shinozaki,K.: "The plant hormone abscisic acid mediates the drought‐induced expression but not the seed‐specific expression of rd22,a gene responsive to dehydration stress in Arabidopsis thaliana." Mol.Gen.Genet.238. 17-25 (1993)
[文献書誌] Urao,T.: "An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence." Plant Cell. 5. 1529-1539 (1993)
[文献書誌] Kiyosue,T.: "Characterization of cDNA for a dehydration‐inducible gene that encodes a clp A,B‐like protein in Arabidopsis thaliana L." Biochem.Biophys.Res.Commun.196. 1214-1220 (1993)
[文献書誌] Koizumi,M.: "Structure and expression of two genes that encode distinct drought‐inducible cysteine protcinascs in Arabidopsis thaliana." Gene. 129. 175-182 (1993)
[文献書誌] Mi,H.: "Electron donation form cyclic and respiratory flows to photosynthetic intersystem chain is mediated by pyridine nucleotid dehydrogenase in the cyanobacterium Synechocy,stis PCC6803." Plant Cell Physiol.33. 1233-1237 (1993)
[文献書誌] Ogawa,T.: "Isolation.and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization" Photosyn.Res.in press. (1993)
[文献書誌] Ogawa,T.: "Cloning of a gene(ccmA)encoding a carboxysomal constituent of Synechocystis PCC6803." J.Bacteriol.in press. (1993)
[文献書誌] Mi,H.: "NAD(P)H dehydrogenase‐dependent cyclic electron flow around photosystem I in the cyanobacterium Synechocystis PCC6803:A study of dark‐starved cells and spheroplasts." Plant Cell Physiol.in press. (1993)
[文献書誌] Wada,M.: "Molecular cloning of P‐type ATPase from marine alga Heterosigma akashiwo." Plant Mol.Biol.in press. (1993)

1993 年度 実績報告書

光合成の環境適応の分子機構

研究代表者

村田 紀夫 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)

研究成果

[文献書誌] Vigh,L.: "The primary signal in the biological perception of temperature:Pd‐catalyzed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803." Proc.Natl.Acad.Sci.U.S.A.90. 9090-9094 (1993)

[文献書誌] Wada,H.: "The contribution of membrane‐lipids to the ability of the photosynthetic machinery to tolerate temperature stress." Proc.Natl.Acad.Sci.U.S.A.in press. (1993)

[文献書誌] Mamedov,Mahir.: "Effects of glycinebetatine and unsaturation of membrane lipids on heat stability of photosynethetic electron‐transport and phosphorylation reactions in Synechocystis PCC6803." Biochem.Biophs.Actra. 1142. 1-5 (1993)

[文献書誌] Nishiyama,Y.: "Photosynthetic adaptation to high temperature associated with thylakoid membranes of Synechococcus PCC7002." Plant Cell Physiol.34. 337-343 (1993)

[文献書誌] Los,D.: "The temperature‐dependent expression of the desaturase gene desA in Synechocystis PCC803." FEBS Letters. 318. 57-60 (1993)

[文献書誌] Wada,H.: "The desA gene of the cyanobacterium Synechocystis sp.strain PCC6803 is the structural gene for DELTA12 desaturase." J.Bacteriol.175. 6056-6058 (1993)

[文献書誌] Sakamoto,T.: "Identification of conserved domains in the DELTA 12 desaturase of cyanobacteria." Plant Mol.Biol.in press. (1993)

[文献書誌] Morisaki,N.: "Effect of side‐chain structure on inhibition of yeast fatty‐acid synthase by cerulenin analogues." Eur.J.Biochem.211. 111-115 (1993)

[文献書誌] Ishitani,M.: "Regulation of glycinebetaine accumulation in the halotolerant cyanobacterium Aphanothece halophytica." Aust.J.Plant Physiol.20. 693-703 (1993)

[文献書誌] Ishitani,M.: "Betainealdehyde dehydrogenase in the gramineae:Levels in leaves of both betaine‐accumulating and nonaccumulating cereal plants ^1." Plant Cell Physiol. 34. 493-495 (1993)

[文献書誌] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of winter and spring bareley plants." Plant,Cell and Environment. 17. 89-95 (1993)

[文献書誌] Aiba,H.: "Sensor and regulator proteins from cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signaltransduction protein families:implication in the adaptive response to phosphate limitation." Mol.Microbiol.8. 81-91 (1993)

[文献書誌] Nagaya,M.: "Cloning of a sensory‐kinase‐encoding gene that belongs to the two‐component regulatory family from the cyanobacterium Synechococcus sp.PCC7942." Gene. 131. 119-124 (1993)

[文献書誌] Kanamura,K.: "The cyanobacterium,Synechococcus sp.PCC7942,possesses two distinct genes encoding cation‐transporting P‐type ATPases." FEBS Letters. 330. 99-104 (1993)

[文献書誌] Nagaya,M.: "A cyanobacterial gene encoding a protein with extensive homology to mammalian phosphoribosylpyrophosphate synthetase." Biosci.Biotech.Biochem.57. 1958-1959 (1993)

[文献書誌] Yamaguchi‐Shinozaki,K.: "Characterization of the expression of a desiccation‐responsive rD29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants." Mol.Gen.Genet.236. 331-340 (1993)

[文献書誌] Yamaguchi‐Shinozaki,K.: "Arabidopsis DNA encoding two desiccation‐responsive rd29 genes^<1.>" Plant Physiol.101. 1119‐1120 (1993)

[文献書誌] Kiyosue,T.: "Characterization of two cDNAs(ERD11 and ERD13)for dehydration‐inducible genes that encode putative glutathione S‐transferases in Arabidopsis thaliana L." FEBS Letters. 335. 189-192 (1993)

[文献書誌] Yamaguchi‐Shinozaki,K.: "The plant hormone abscisic acid mediates the drought‐induced expression but not the seed‐specific expression of rd22,a gene responsive to dehydration stress in Arabidopsis thaliana." Mol.Gen.Genet.238. 17-25 (1993)

[文献書誌] Urao,T.: "An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence." Plant Cell. 5. 1529-1539 (1993)

[文献書誌] Kiyosue,T.: "Characterization of cDNA for a dehydration‐inducible gene that encodes a clp A,B‐like protein in Arabidopsis thaliana L." Biochem.Biophys.Res.Commun.196. 1214-1220 (1993)

[文献書誌] Koizumi,M.: "Structure and expression of two genes that encode distinct drought‐inducible cysteine protcinascs in Arabidopsis thaliana." Gene. 129. 175-182 (1993)

[文献書誌] Mi,H.: "Electron donation form cyclic and respiratory flows to photosynthetic intersystem chain is mediated by pyridine nucleotid dehydrogenase in the cyanobacterium Synechocy,stis PCC6803." Plant Cell Physiol.33. 1233-1237 (1993)

[文献書誌] Ogawa,T.: "Isolation.and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization" Photosyn.Res.in press. (1993)

[文献書誌] Ogawa,T.: "Cloning of a gene(ccmA)encoding a carboxysomal constituent of Synechocystis PCC6803." J.Bacteriol.in press. (1993)

[文献書誌] Mi,H.: "NAD(P)H dehydrogenase‐dependent cyclic electron flow around photosystem I in the cyanobacterium Synechocystis PCC6803:A study of dark‐starved cells and spheroplasts." Plant Cell Physiol.in press. (1993)

[文献書誌] Wada,M.: "Molecular cloning of P‐type ATPase from marine alga Heterosigma akashiwo." Plant Mol.Biol.in press. (1993)

1993 年度実績報告書

村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)