1993 年度実績報告書

標的遺伝子組換えによる生体機能の研究

研究課題

研究課題/領域番号	05275101
研究機関	九州大学
研究代表者	勝木元也九州大学, 生体防御医学研究所, 教授 (20051732)
研究分担者	関口睦夫九州大学, 生体防御医学研究所, 所長 (00037342) 中辻憲夫国立遺伝学研究所, 教授 (80237312) 近藤寿人大阪大学, 細胞生体工学センター, 教授 (70127083) 相沢慎一理化学研究所ライフサイエンス筑波研究センター, 副主任研究員 (60073011) 山村研一熊本大学, 医学部, 教授 (90115197)
キーワード	標的遺伝子組換え / ジーンターゲッティング / グルタミン酸受容体 / セロトニン受容体 / p53 / GFAP / myogenin / tau
研究概要	本研究は最近著しく進歩した標的遺伝子組換え法を用いて,目的とする遺伝子を自在に構造変化させ,それに伴なう生体の形質転換を通して,現代のバイオサイエンスの大きな課題の一つである個体レベルでの遺伝子機能の研究を目的とすす。総括班である本班は,2つの計画研究班と11人の公募研究班員の研究を評価し,成果が所期の目標に向かっているかを検討し,班員相互の協力体制を励まし,方法や資材において共有できるよう指導する役割を荷っている。これらの目的を達成するために,ニュースレターの作製と配布,幹事会の開催,班員の研究現場への相互訪問,班会議および重点領域研究「形態形成」との共同ワークショップの開催を行なった。また,本研究は,そのユニークな方法論に大きな価値があることから,日本において将来ともに充実したシステムを数ケ所に設定する必要性が論じられた。そこで,総括班から重点配分として,九大,熊大,理化研に配分され,それぞれ多くの標的組換えマウス(ジーンターゲッティングマウス)が作成された。本研究によって得られた遺伝子標的マウスを列挙すれば,グルタミン酸受容体(NMDAタイプ5種,メタボトロピックタイプ6種) セロトニン受容体,p53,pou領域,tau,GFAP,免疫グロブリン遺伝子,APC,myogeninなどすでに20種を越えている。これらのマウスを作成する力が,日本中にこの一年間で大きく広がったと考えられる。

研究成果

(24件)

すべてその他

すべて文献書誌 (24件)

[文献書誌] Akiyama,M. Katsuki,M.,et al.: "Lymphocyte infiltration of the skin in transgenic mice carrying the human interleukin-2 gene." Arch Dermatol Res. 285. 379-384 (1993)
[文献書誌] Takaori,K. Katsuki,M.,et al.: "Biochemical characteristics of human renin expressed in transgenic mice." Clinical Science. 84. 21-29 (1993)
[文献書誌] Hayashizaki,Y. Katsuki,M.,et al.: "Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using RLGS method." Nature Genetics. 6. 33-40 (1993)
[文献書誌] Miyado,K. Katsuki,M.,et al.: "Isolation of a yeast cDNA clone that encodes a novel transmembrane protein having a c-terminal highly basic region." Biochem Biophys Res Commun. in press (1994)
[文献書誌] Furuta,Y. Aizawa,S.,et al.: "MDS-like experimental myelodysplasia:multilineage abnormal hematopoiesis in transgenic mice harboring the SV40 large T antigen under an immunoglobulin enhancer." Exp Hematol. 806-815 (1993)
[文献書誌] Nada,S. Aizawa,S.,et al.: "Constitutive activation of Src family kinases in mouse embryos that lack Csk.interleukin-2 gene." Cell. 73. 1125-1135 (1993)
[文献書誌] Furuta,Y Aizawa,S.,et al.: "Degeneration of skeletal and cardiac muscles in c-myb transgenic mice.interleukin-2 gene." Transgenic Res. 2. 199-207 (1993)
[文献書誌] Yagi,T. Aizawa,S.,et al.: "A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene." Anal Biochiem. 214. 77-86 (1993)
[文献書誌] Yagi,T. Aizawa,S.,et al.: "A role for Fin tyrosine kinase in the suckling behavior of neoonatal mice." Nature. 366. 742-745 (1993)
[文献書誌] Wakamatsu,Y. Kondoh,H.,et al.: "Transition of localization of the n-myc protein from nucleus to cytoplasm in differentiating neurons." Neuron. 10. 1-9 (1993)
[文献書誌] Sawai,S. Kondoh,H.,et al.: "Defects of embryonic organogenesis resulting from targeted desruption of the N-myc gene in the mouse." Development. 117. 1445-1455 (1993)
[文献書誌] Kamachi,Y. Kondoh,H.,et al.: "Overlapping positive and negative elements determine lens specificity of the delta1-crystallin enhancer." Mol Cell Biol. 13. 5206-5213 (1993)
[文献書誌] Tomotsune,D. Kondoh,H.,et al.: "A mouse homologue of the Drosophila tumor-suppressor gene 1(2)gl controlled by Hox-C8 in vivo." Nature. 365. 69-72 (1993)
[文献書誌] Funahashi,J. Kondoh,H.,et al.: "delta Crystallin enhancer binding protein dEF1 is a zinc finger-homeodomain protein implicated in post gastrulation embryogenesis." Development. 119. 433-446 (1993)
[文献書誌] Nagata.I. Nakatsuji,N.,et al.: "Perpendicular contact guidance of CNS neuroblasts on artificial microstructures." Development. 117. 401-408 (1993)
[文献書誌] Hashimoto,S. Nakatsuji,N.,et al.: "Promotion of cell adhesion on fibronectin during adenovirus infection of KB cells." Exp Cell Res. 205. 270-275 (1993)
[文献書誌] Kawase,E. Nakatsuji,N.,et al.: "Tumor necrosis factor (TNF)-alpha stimulates proliferation of mouse primordial germ cells in culture" Dev Biol. (in press). (1994)
[文献書誌] Kawase,E. Nakatsuji,N.,et al.: "Strain difference in establishment of mouse embryonic stem (ES) cell lines." Int J Devel Biol. (in press). (1994)
[文献書誌] Niwa,H. Yamamura,K.,et al: "An efficient gene-trap method using poli A trap vectors and characterization of gene-trap events." J Biochem. 113. 343-349 (1993)
[文献書誌] Nakata,K. Yamamura,K.,et al: "Osteoarthritis associated with mild chondrodysplasia in transgenic mice expres-sing alpha(IX) collagen chains with a central deletion." Proc Natl Acad Sci USA. 90. 2870-2874 (1993)
[文献書誌] Yamamura,K.,et al: "Transgenic mouse model for human genetic diseases." Mol Reprod Dev. 36. 248-250 (1993)
[文献書誌] Kato,K. Yamamura,K.,et al.: "Use of the hemagglutinating virus of Japan(HVJ)-liposome method for analysis of infiltrating lymphocytes induced by hepatitis B virus gene expression in liver tissue." Biochem Biophys Acta. 1182. 283-290 (1993)
[文献書誌] 山村研一: "発生工学のすすめ実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)
[文献書誌] 中辻憲夫: "発生工学のすすめ実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)

1993 年度 実績報告書

標的遺伝子組換えによる生体機能の研究

研究代表者

勝木 元也 九州大学, 生体防御医学研究所, 教授 (20051732)

研究成果

[文献書誌] Akiyama,M. Katsuki,M.,et al.: "Lymphocyte infiltration of the skin in transgenic mice carrying the human interleukin-2 gene." Arch Dermatol Res. 285. 379-384 (1993)

[文献書誌] Takaori,K. Katsuki,M.,et al.: "Biochemical characteristics of human renin expressed in transgenic mice." Clinical Science. 84. 21-29 (1993)

[文献書誌] Hayashizaki,Y. Katsuki,M.,et al.: "Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using RLGS method." Nature Genetics. 6. 33-40 (1993)

[文献書誌] Miyado,K. Katsuki,M.,et al.: "Isolation of a yeast cDNA clone that encodes a novel transmembrane protein having a c-terminal highly basic region." Biochem Biophys Res Commun. in press (1994)

[文献書誌] Furuta,Y. Aizawa,S.,et al.: "MDS-like experimental myelodysplasia:multilineage abnormal hematopoiesis in transgenic mice harboring the SV40 large T antigen under an immunoglobulin enhancer." Exp Hematol. 806-815 (1993)

[文献書誌] Nada,S. Aizawa,S.,et al.: "Constitutive activation of Src family kinases in mouse embryos that lack Csk.interleukin-2 gene." Cell. 73. 1125-1135 (1993)

[文献書誌] Furuta,Y Aizawa,S.,et al.: "Degeneration of skeletal and cardiac muscles in c-myb transgenic mice.interleukin-2 gene." Transgenic Res. 2. 199-207 (1993)

[文献書誌] Yagi,T. Aizawa,S.,et al.: "A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene." Anal Biochiem. 214. 77-86 (1993)

[文献書誌] Yagi,T. Aizawa,S.,et al.: "A role for Fin tyrosine kinase in the suckling behavior of neoonatal mice." Nature. 366. 742-745 (1993)

[文献書誌] Wakamatsu,Y. Kondoh,H.,et al.: "Transition of localization of the n-myc protein from nucleus to cytoplasm in differentiating neurons." Neuron. 10. 1-9 (1993)

[文献書誌] Sawai,S. Kondoh,H.,et al.: "Defects of embryonic organogenesis resulting from targeted desruption of the N-myc gene in the mouse." Development. 117. 1445-1455 (1993)

[文献書誌] Kamachi,Y. Kondoh,H.,et al.: "Overlapping positive and negative elements determine lens specificity of the delta1-crystallin enhancer." Mol Cell Biol. 13. 5206-5213 (1993)

[文献書誌] Tomotsune,D. Kondoh,H.,et al.: "A mouse homologue of the Drosophila tumor-suppressor gene 1(2)gl controlled by Hox-C8 in vivo." Nature. 365. 69-72 (1993)

[文献書誌] Funahashi,J. Kondoh,H.,et al.: "delta Crystallin enhancer binding protein dEF1 is a zinc finger-homeodomain protein implicated in post gastrulation embryogenesis." Development. 119. 433-446 (1993)

[文献書誌] Nagata.I. Nakatsuji,N.,et al.: "Perpendicular contact guidance of CNS neuroblasts on artificial microstructures." Development. 117. 401-408 (1993)

[文献書誌] Hashimoto,S. Nakatsuji,N.,et al.: "Promotion of cell adhesion on fibronectin during adenovirus infection of KB cells." Exp Cell Res. 205. 270-275 (1993)

[文献書誌] Kawase,E. Nakatsuji,N.,et al.: "Tumor necrosis factor (TNF)-alpha stimulates proliferation of mouse primordial germ cells in culture" Dev Biol. (in press). (1994)

[文献書誌] Kawase,E. Nakatsuji,N.,et al.: "Strain difference in establishment of mouse embryonic stem (ES) cell lines." Int J Devel Biol. (in press). (1994)

[文献書誌] Niwa,H. Yamamura,K.,et al: "An efficient gene-trap method using poli A trap vectors and characterization of gene-trap events." J Biochem. 113. 343-349 (1993)

[文献書誌] Nakata,K. Yamamura,K.,et al: "Osteoarthritis associated with mild chondrodysplasia in transgenic mice expres-sing alpha(IX) collagen chains with a central deletion." Proc Natl Acad Sci USA. 90. 2870-2874 (1993)

[文献書誌] Yamamura,K.,et al: "Transgenic mouse model for human genetic diseases." Mol Reprod Dev. 36. 248-250 (1993)

[文献書誌] Kato,K. Yamamura,K.,et al.: "Use of the hemagglutinating virus of Japan(HVJ)-liposome method for analysis of infiltrating lymphocytes induced by hepatitis B virus gene expression in liver tissue." Biochem Biophys Acta. 1182. 283-290 (1993)

[文献書誌] 山村研一: "発生工学のすすめ 実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)

[文献書誌] 中辻憲夫: "発生工学のすすめ 実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)

1993 年度実績報告書

勝木元也九州大学, 生体防御医学研究所, 教授 (20051732)

[文献書誌] 山村研一: "発生工学のすすめ実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)

[文献書誌] 中辻憲夫: "発生工学のすすめ実験医学バイオサイエンスシリーズ 11" 羊土社, 116 (1993)