1993 年度実績報告書

植物細胞形態構築の制御機構

研究課題

研究課題/領域番号	05276103
研究機関	大阪大学
研究代表者	柴岡弘郎大阪大学, 理学部, 教授 (60087054)
研究分担者	福田裕穂東北大学, 理学部, 助教授 (10165293) 馳沢盛一郎東京大学, 大学院・理学系研究科, 講師 (40172902) 園部誠司大阪大学, 理学部, 助手 (30197024) 保尊隆享大阪市立大学, 理学部, 助教授 (70135771) 林隆久京都大学, 木貭科学研究所, 助教授 (70231529)
キーワード	植物ホルモン / 細胞形態形成 / 細胞伸長 / 細胞分化 / 細胞壁 / 微小管 / エンドグルカナーゼ / cDNA
研究概要	発達した細胞壁に囲まれた細胞により構築されている植物の形は細胞一個一個の形に大きく依存している。植物ホルモンは細胞伸長そのもの、および細胞伸長の方向を制御することにより細胞一個一個の形を制御し、ひいては植物の形に大きな影響を与えている。本研究は植物ホルモンの細胞伸長制御機構、および伸長方向制御機構の解明に重点を置き行われたものである。植物ホルモンによる細胞伸長方向の制御には細胞質表層微小管が関与している。柴岡は、アズキ上胚軸におけるジベレリンによる表層微小管の方向変更の過程に、ジベレリンによる遺伝子発現の過程が含まれていることを示した。馳沢は、タバコ培養細胞を用い表層微小管の配列制御に重要な役割を演じていると考えられる微小管重合中心蛋白質の有力な候補を見いだし、そのcDNAクローンを得ることに成功した。園部は、表層微小管の配列制御機構を理解するには表層微小管に付随している蛋白質の性質を知ることが不可欠であるとの認識に立ち、まずタバコ培養細胞の表層微小管の単離を行い、これに付随する4種類の蛋白質を見いだした。オーキシンによる細胞伸長は細胞壁中のキシログルカンの分解によりもたらされると考えられる。林は、ポプラの培養細胞を用い、キシログルカンの分解を触媒するエンド-1,4-β-グルカナーゼのcDNAの単離に成功した。保尊もキシログルカンの分解を引き起こす細胞壁酵素をアズキ上胚軸より単離した。保尊はまた、キシログルカンの分解を調節する因子が細胞壁中に存在することを見いだした。植物の形を内部構造も含めて考えるなら、細胞分化の過程の解明をおろそかにすることは出来ない。ヒャクニチソウ葉肉細胞をオーキシンとサイトカイニン存在下で培養すると管状要素に分化するが、福田は、分化の過程でサイトカイニンにより制御を受ける可能性のあるcDNAクローンを得た。

研究成果
(26件)

すべてその他

すべて文献書誌 (26件)

[文献書誌] Sakiyama-Sogo,M.: "Gibberellil A_3 and abscisic acid cause the reorientation of cortical microtubules in epicotyl cells of the decapitated dwarf pea." Plant Cell Physiol.34. 431-437 (1993)
[文献書誌] Kaneta,T.: "Actinomycin D inhibits the GA_3-induced elongation of azuki bean epicotyls and the reorientation of cortical microtubules." Plant Cell Physiol.34. 1125-1132 (1993)
[文献書誌] Shibaoka,H.: "The use of BY-2 cells for studies of the plant cytoskeleton." J.Plant Res.Special Issue. 3. 3-15 (1993)
[文献書誌] Shibaoka,H.: "Regulation by gibberellins of the orientation of cortical microtubules in plant cells." Aust.J.Plant Physiol.20. 461-470 (1993)
[文献書誌] Karahara,I.: "The casparian strip in pea epicotyls:effects of light on its development." Planta. 192. 269-275 (1993)
[文献書誌] Shibaoka,H.: "The plant cytoskeleton." Curr.Opin.Cell Biol.6. 10-15 (1994)
[文献書誌] Hayashi,T.: "Macromolecular complexes of xyloglucan and cellulose obtained by annealing." Plant Cell Physiol.(in press).
[文献書誌] Hoson,T.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl seqments." Physiol.Plant.87. 142-147 (1993)
[文献書誌] Hoson,T.: "Regulation of polysaccharide breakdown during auxin-induced cell wall loosening." J.Plant Res.106. 369-381 (1993)
[文献書誌] Hoson,T.: "Abscisic acid content in air-and water-grown rice coleoptiles." J.Plant Physiol.142. 593-596 (1993)
[文献書誌] Jiang,C.J.: "Identification and preliminary characterization of a 65 kDa higher-plant microtubule-associated protein." J.Cell Sci.105. 891-901 (1993)
[文献書誌] Sonobe,S.: "Association of microtubules with the plasma membrane of tobacco BY-2 cells in vitro." Plant Cell Physiol.(in press).
[文献書誌] Hamada,H.: "Stabilization of cortical microtubules in maize mesocotyl cells by gibberellin A_3." Plant Cell Physiol.35(in press).
[文献書誌] Nakamura,S.: "Production of endo-1,4-β-glucanase activity:A simple method for suspension culture poplar cells." Biosci.Biotech.Biochem.57. 1933-1934 (1993)
[文献書誌] Fry,S.C.: "An unambiguous nomenclature for xyloglucan-derived oligosaccharides." Physiol.Plant.89. 1-3 (1993)
[文献書誌] Nakamura,S.: "Occurrence of endo-1,4-β-glucanase activities in suspension-cultured poplar cells during growth." Mokuzai Gakkaishi. 39. 1056-1061 (1993)
[文献書誌] Nakamura,S.: "Purification and properties of extracellular endo-1,4-β-glucanase from suspension-cultured poplar cells." Plant Cell Physiol.34. 1009-1013 (1993)
[文献書誌] Baba,K.: "Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems." Plant Cell Physiol.(in press).
[文献書誌] Nagata,T.: "Events at the border between the M and G1 phases in highly synchronized culture of tobacco BY-2 cells." J.Plant Res.Special Issue. 3. 29-35 (1993)
[文献書誌] Hasezawa,S.: "Microtubule organizing centers in plant cells:localization of a 49 kDa protein that is immunologically cross-reactive to a 51 kDa protein from sea urchin centrosomes in synchronized tobacco BY-2 cells" Protoplasma. 176. 64-74 (1993)
[文献書誌] Nagata,T.: "The origin and organization of cortical microtubules during the transition between M and G2 phases of the cell cycle as observed in highly synchronized cells of tobacco BY-2." Planta. (in press).
[文献書誌] 馳沢盛一郎: "細胞周期における細胞骨格のダイナミクス" 遺伝. 47. 12-17 (1993)
[文献書誌] Fukuda,H.: "Molecular mechanism of xylem differentiation." J.Plant Res.Special Issue. 3. 97-107 (1993)
[文献書誌] Sato,Y.: "Interrelationship between lignin deposition and the activities of peroxidase isozymes in differentiating tracheary elements of Zinnia:Analysis with L-a-aminooxy-b-phenylpropionic acid and 2-aminoindan-2-phosphonic acid." Planta. 189. 584-589 (1993)
[文献書誌] Demura,T.: "Molecular cloning and characterization of cDNAs associated with tracheary element differentiation in cultured Zinnia cells." Plant Physiol.103. 815-821 (1993)
[文献書誌] Fukuda,H.: "Redifferentiation of single mesophyll cells into tracheary elements." Int.J.Plant Sci.(in press). (1994)

1993 年度 実績報告書

植物細胞形態構築の制御機構

研究代表者

柴岡 弘郎 大阪大学, 理学部, 教授 (60087054)

研究成果

[文献書誌] Sakiyama-Sogo,M.: "Gibberellil A_3 and abscisic acid cause the reorientation of cortical microtubules in epicotyl cells of the decapitated dwarf pea." Plant Cell Physiol.34. 431-437 (1993)

[文献書誌] Kaneta,T.: "Actinomycin D inhibits the GA_3-induced elongation of azuki bean epicotyls and the reorientation of cortical microtubules." Plant Cell Physiol.34. 1125-1132 (1993)

[文献書誌] Shibaoka,H.: "The use of BY-2 cells for studies of the plant cytoskeleton." J.Plant Res.Special Issue. 3. 3-15 (1993)

[文献書誌] Shibaoka,H.: "Regulation by gibberellins of the orientation of cortical microtubules in plant cells." Aust.J.Plant Physiol.20. 461-470 (1993)

[文献書誌] Karahara,I.: "The casparian strip in pea epicotyls:effects of light on its development." Planta. 192. 269-275 (1993)

[文献書誌] Shibaoka,H.: "The plant cytoskeleton." Curr.Opin.Cell Biol.6. 10-15 (1994)

[文献書誌] Hayashi,T.: "Macromolecular complexes of xyloglucan and cellulose obtained by annealing." Plant Cell Physiol.(in press).

[文献書誌] Hoson,T.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl seqments." Physiol.Plant.87. 142-147 (1993)

[文献書誌] Hoson,T.: "Regulation of polysaccharide breakdown during auxin-induced cell wall loosening." J.Plant Res.106. 369-381 (1993)

[文献書誌] Hoson,T.: "Abscisic acid content in air-and water-grown rice coleoptiles." J.Plant Physiol.142. 593-596 (1993)

[文献書誌] Jiang,C.J.: "Identification and preliminary characterization of a 65 kDa higher-plant microtubule-associated protein." J.Cell Sci.105. 891-901 (1993)

[文献書誌] Sonobe,S.: "Association of microtubules with the plasma membrane of tobacco BY-2 cells in vitro." Plant Cell Physiol.(in press).

[文献書誌] Hamada,H.: "Stabilization of cortical microtubules in maize mesocotyl cells by gibberellin A_3." Plant Cell Physiol.35(in press).

[文献書誌] Nakamura,S.: "Production of endo-1,4-β-glucanase activity:A simple method for suspension culture poplar cells." Biosci.Biotech.Biochem.57. 1933-1934 (1993)

[文献書誌] Fry,S.C.: "An unambiguous nomenclature for xyloglucan-derived oligosaccharides." Physiol.Plant.89. 1-3 (1993)

[文献書誌] Nakamura,S.: "Occurrence of endo-1,4-β-glucanase activities in suspension-cultured poplar cells during growth." Mokuzai Gakkaishi. 39. 1056-1061 (1993)

[文献書誌] Nakamura,S.: "Purification and properties of extracellular endo-1,4-β-glucanase from suspension-cultured poplar cells." Plant Cell Physiol.34. 1009-1013 (1993)

[文献書誌] Baba,K.: "Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems." Plant Cell Physiol.(in press).

[文献書誌] Nagata,T.: "Events at the border between the M and G1 phases in highly synchronized culture of tobacco BY-2 cells." J.Plant Res.Special Issue. 3. 29-35 (1993)

[文献書誌] Hasezawa,S.: "Microtubule organizing centers in plant cells:localization of a 49 kDa protein that is immunologically cross-reactive to a 51 kDa protein from sea urchin centrosomes in synchronized tobacco BY-2 cells" Protoplasma. 176. 64-74 (1993)

[文献書誌] Nagata,T.: "The origin and organization of cortical microtubules during the transition between M and G2 phases of the cell cycle as observed in highly synchronized cells of tobacco BY-2." Planta. (in press).

[文献書誌] 馳沢盛一郎: "細胞周期における細胞骨格のダイナミクス" 遺伝. 47. 12-17 (1993)

[文献書誌] Fukuda,H.: "Molecular mechanism of xylem differentiation." J.Plant Res.Special Issue. 3. 97-107 (1993)

[文献書誌] Sato,Y.: "Interrelationship between lignin deposition and the activities of peroxidase isozymes in differentiating tracheary elements of Zinnia:Analysis with L-a-aminooxy-b-phenylpropionic acid and 2-aminoindan-2-phosphonic acid." Planta. 189. 584-589 (1993)

[文献書誌] Demura,T.: "Molecular cloning and characterization of cDNAs associated with tracheary element differentiation in cultured Zinnia cells." Plant Physiol.103. 815-821 (1993)

[文献書誌] Fukuda,H.: "Redifferentiation of single mesophyll cells into tracheary elements." Int.J.Plant Sci.(in press). (1994)

1993 年度実績報告書

柴岡弘郎大阪大学, 理学部, 教授 (60087054)