1994 年度実績報告書

植物細胞形態構築の制御機構

研究課題

研究課題/領域番号	05276103
研究機関	大阪大学
研究代表者	柴岡弘郎大阪大学, 理学部, 教授 (60087054)
研究分担者	福田裕穂東北大学, 理学部, 教授 (10165293) 西谷和彦鹿児島大学, 教養部, 助教授 (60164555) 保尊隆亨大阪市立大学, 理学部, 助教授 (70135771) 馳沢盛一郎東京大学, 大学院・理学系研究科, 助教授 (40172902) 園部誠司姫路工業大学, 理学部, 助教授 (30197024)
キーワード	植物ホルモン / 細胞形態形成 / 細胞伸長 / 細胞壁 / 微小管 / 細胞分化 / 遺伝子発現 / 細胞質分裂
研究概要	本研究は植物細胞の伸長制御、伸長方向制御、細胞質分裂制御などの機構の解明を目的として行われた。細胞伸長方向の制御には細胞質表層微小管が関与している。柴岡は、アズキ上胚軸において、ブラシノライドが横方向の微小管を持つ細胞の割合を増加させること、蛋白質リン酸化阻害剤が表層微小管の横方向から縦方向への変換を促進するることを明らかにした。また、植物の細胞質分裂装置である隔膜形成体から隔膜形成体形成に関与していると思われるモーター蛋白質の単離に成功した。園部は、表層微小管の配列制御機構を理解する上で表層微小管付髄蛋白質の性質を知ることが不可欠と考え、タバコ培養細胞から表層微小管付髄蛋白質4種類を単離し、その中の80および65kDa蛋白質の一部アミノ酸配列を明らかにした。馳沢は、表層微小管の配列制御に関与していると考えられる蛋白質のcDNAの全塩基配列を決定し、EF1α様蛋白質であること、精製した蛋白質が微小管重合能を有することを示した。オーキシンによる細胞伸長は細胞壁中のキシログルカンの分解によるものと考えられる。林は、ポプラ培養細胞を用い、エンド-1,4-β-グルカナーゼのmRNAレベルがオーキシンにより上昇することを見いだした。保尊は、アズキ上胚軸からキシログルカンを分子量数万の大きさに切断する新規エンドグルカナーゼを単離した。西谷は、シロイヌナズナより得たエンド・キシログルカン転移酵素をコードするゲノムDNAのプロモーター領域にジベレリンに反応する配列が存在することを明らかにした。福田はオーキシンとサイトカイニン存在下で培養する管状要素に分化する単離ヒャクニチソウ葉肉細胞を用い、分化初期に発現するcDNAクローン17個を単離した。

研究成果
(36件)

すべてその他

すべて文献書誌 (36件)

[文献書誌] KARAHARA,T.: "The casparian strip in pea epicotyls:effects of light on its development" Planta. 192. 269-275 (1994)
[文献書誌] HAMADA,H.: "Stabilization of cortical microtubules in maize mesocotyl cells by gibberellin A_3." Plant and Cell Physiology. 35. 189-196 (1994)
[文献書誌] ASADA,T.: "Isolation of polypeptides with microtubule-translocating activity from phragmoplasts of tobacco BY-2 cells" Journal of Cell Science. 107. 2249-2257 (1994)
[文献書誌] SHIBAOKA,H.: "The plant cytoskeleton" Current Opinion in Cell Biology. 6. 10-15 (1994)
[文献書誌] SHIBAOKA,H.: "Plant hormone-induced changes in the orientation of cortical microtubules" Annu.Rev.Plant.Physiol.Plant Mol.Biol.45. 527-544 (1994)
[文献書誌] 柴岡弘郎: "植物の形の制御と微小管" 蛋白質・核酸・酵素. 40. 33-42 (1995)
[文献書誌] MAYUMI,K.: "A possible double role for brassinolide in the reorientation of cortical microtubules in the epidermal cells of Azuki bean epicotyls" Plant and Cell Physiology. 36. 173-181 (1995)
[文献書誌] SONOBE,S.: "Association of microtubules with the plasma membrane of tobacco BY-2 cells in vitro" Plant and Cell Physiology. 35. 451-460 (1994)
[文献書誌] NAGATA,T.: "The origin and organization of cortical microtubules during the transition from M and G_1 phases of the cell cycle as observed in highly synchronized cells of tobacco BY-2" Planta. 193. 567-572 (1994)
[文献書誌] NAGATA,T.: "Genes involved in the dedifferentiation of plant cells" Intern.J.Develop.Biology. 38. 109-138 (1994)
[文献書誌] HASEZAWA,S.: "Oblique cell plate formation in tobacco BY-2 cells originates in double preprophase bands" Journal of Plant Research. 107. 355-359 (1994)
[文献書誌] TAKAHASHI,Y.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear organization of its gene products" Planta. (in press).
[文献書誌] HAYASHI,T.: "Characterization of the adsorption of xyloglucan to cellulose" Plant and Cell Physiology. 35. 1199-1205 (1994)
[文献書誌] OMIYA,Y.: "Endo-1,4-β-glucomase in suspension-cultured poplar cells" Wood Research. 81. 5-7 (1994)
[文献書誌] HAYASHI,T.: "Effects of the degree of polymerization on the binding of xyloglucans to cellulose" Plant and Cell Physiology. 35. 893-899 (1994)
[文献書誌] OHSUMI,C.: "The oligosaccharide units of the xyloglucans in the cell walls of bulbs of onion,garlic and their hybrid" Plant and Cell Physiology. 35. 963-967 (1994)
[文献書誌] HAYASHI,T.: "Compositional analysis of the oligosaccharide units of xyloglucan from suspension cultured poplar cells" Biosci.Biotech.Biochem.58. 1707-1708 (1994)
[文献書誌] HAYASHI,T.: "Effects of amino acid medium on cell aggregation in suspension-cultured rice cells." Biosci.Biotech.Biochem.58. 256-260 (1994)
[文献書誌] NISHITANI,K.: "Endo-xyloglucan transferase,a new class of transferase involved in cell wall construction" J.Plant Research. (in press).
[文献書誌] 西谷和彦: "細胞壁構築に関与する新しい酵素-エンドキシログルカン転移酵素" 植物細胞工学. 6. 130-136 (1994)
[文献書誌] FUKUDA,H.: "Redifferentiation of single mesophyll cells into tracheary elements" Intern.J.Plant Science. 155. 262-271 (1994)
[文献書誌] SUGIYAMA,M: "Characteristics of the inhibitory effects of aphidicolin on transdifferm tiation into tracheary elements of isolated mesophyll cells of Zinnia elegans" Plant and Cell Physiology. 35. 519-522 (1994)
[文献書誌] DEMURA,T.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant Cell. 6. 967-981 (1994)
[文献書誌] ITO,M.: "Meristem-specific gene expression directed by the promotor of the S-phase-specific gene,cyco7" Plant Molecular Biology. 24. 863-878 (1994)
[文献書誌] HAYASHI,T.: "Endo-1,4-β-glucanase in the cell wall of stems of auxintreated pea seedlings" Plant and Cell Physiology. 35. 419-424 (1994)
[文献書誌] BABA,K.: "Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems" Plant and Cell Physiology. 35. 439-444 (1994)
[文献書誌] HAYASHI,T.: "Macromolecular complexes of xyloglucan and cellulose obtained by annealing" Plant and Cell Physiology. 35. 219-223 (1994)
[文献書誌] OHSUMI,C.: "Carbohydrate analysis of an interspecific hybrid between onion and garlic" Biosci.Biotech.Biochem.58. 959-960 (1994)
[文献書誌] KATO,Y.: "Involvement of phenolic esters in cell aggregation of suspension-cultured rice cells" Plant Physiology. 104. 147-152 (1994)
[文献書誌] HOSON,T.: "Concanavalin A inhibits auxin-inducod elongation and hreakdown of(1→3),(1→4)-β-D-glucan in segments of rice coleoptiles" Plant and Cell Physiology. (in press).
[文献書誌] KOBAYASHI,H.: "Involvement of calmodulin and calmodulin-binding proteins in the differentiation of tracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)
[文献書誌] KAWAHARA,R.: "Analysis by in situ hybridization of the expression of elongation factor 1a in the carrot cells during somatic embryogenesis" J.Plant Research. 107. 361-364 (1994)
[文献書誌] FUKUDA,H.: "Mechanisms of the proliferation and differentiation of plant cell culture systems" Intern.J.Developmental Biology. 38. 287-299 (1994)
[文献書誌] SATO,Y.: "Separation and charactorization of the isozymes of wall-bound peroxidase from cultured Zinnia cells during tracheary element differentiation" Planta. (in press).
[文献書誌] KAWAHARA,R.: "Isolation and characterization of homeobox-containing genes of carrot" Plant Molecular Biology. (in press).
[文献書誌] JOY,R.W.IV: "Cloning and characterization of polyphenol oxidase cDNA of Phytolacca americana" Plant Physiology. (in press).

1994 年度 実績報告書

植物細胞形態構築の制御機構

研究代表者

柴岡 弘郎 大阪大学, 理学部, 教授 (60087054)

研究成果

[文献書誌] KARAHARA,T.: "The casparian strip in pea epicotyls:effects of light on its development" Planta. 192. 269-275 (1994)

[文献書誌] HAMADA,H.: "Stabilization of cortical microtubules in maize mesocotyl cells by gibberellin A_3." Plant and Cell Physiology. 35. 189-196 (1994)

[文献書誌] ASADA,T.: "Isolation of polypeptides with microtubule-translocating activity from phragmoplasts of tobacco BY-2 cells" Journal of Cell Science. 107. 2249-2257 (1994)

[文献書誌] SHIBAOKA,H.: "The plant cytoskeleton" Current Opinion in Cell Biology. 6. 10-15 (1994)

[文献書誌] SHIBAOKA,H.: "Plant hormone-induced changes in the orientation of cortical microtubules" Annu.Rev.Plant.Physiol.Plant Mol.Biol.45. 527-544 (1994)

[文献書誌] 柴岡弘郎: "植物の形の制御と微小管" 蛋白質・核酸・酵素. 40. 33-42 (1995)

[文献書誌] MAYUMI,K.: "A possible double role for brassinolide in the reorientation of cortical microtubules in the epidermal cells of Azuki bean epicotyls" Plant and Cell Physiology. 36. 173-181 (1995)

[文献書誌] SONOBE,S.: "Association of microtubules with the plasma membrane of tobacco BY-2 cells in vitro" Plant and Cell Physiology. 35. 451-460 (1994)

[文献書誌] NAGATA,T.: "The origin and organization of cortical microtubules during the transition from M and G_1 phases of the cell cycle as observed in highly synchronized cells of tobacco BY-2" Planta. 193. 567-572 (1994)

[文献書誌] NAGATA,T.: "Genes involved in the dedifferentiation of plant cells" Intern.J.Develop.Biology. 38. 109-138 (1994)

[文献書誌] HASEZAWA,S.: "Oblique cell plate formation in tobacco BY-2 cells originates in double preprophase bands" Journal of Plant Research. 107. 355-359 (1994)

[文献書誌] TAKAHASHI,Y.: "Expression of the auxin-regulated parA gene in transgenic tobacco and nuclear organization of its gene products" Planta. (in press).

[文献書誌] HAYASHI,T.: "Characterization of the adsorption of xyloglucan to cellulose" Plant and Cell Physiology. 35. 1199-1205 (1994)

[文献書誌] OMIYA,Y.: "Endo-1,4-β-glucomase in suspension-cultured poplar cells" Wood Research. 81. 5-7 (1994)

[文献書誌] HAYASHI,T.: "Effects of the degree of polymerization on the binding of xyloglucans to cellulose" Plant and Cell Physiology. 35. 893-899 (1994)

[文献書誌] OHSUMI,C.: "The oligosaccharide units of the xyloglucans in the cell walls of bulbs of onion,garlic and their hybrid" Plant and Cell Physiology. 35. 963-967 (1994)

[文献書誌] HAYASHI,T.: "Compositional analysis of the oligosaccharide units of xyloglucan from suspension cultured poplar cells" Biosci.Biotech.Biochem.58. 1707-1708 (1994)

[文献書誌] HAYASHI,T.: "Effects of amino acid medium on cell aggregation in suspension-cultured rice cells." Biosci.Biotech.Biochem.58. 256-260 (1994)

[文献書誌] NISHITANI,K.: "Endo-xyloglucan transferase,a new class of transferase involved in cell wall construction" J.Plant Research. (in press).

[文献書誌] 西谷和彦: "細胞壁構築に関与する新しい酵素-エンドキシログルカン転移酵素" 植物細胞工学. 6. 130-136 (1994)

[文献書誌] FUKUDA,H.: "Redifferentiation of single mesophyll cells into tracheary elements" Intern.J.Plant Science. 155. 262-271 (1994)

[文献書誌] SUGIYAMA,M: "Characteristics of the inhibitory effects of aphidicolin on transdifferm tiation into tracheary elements of isolated mesophyll cells of Zinnia elegans" Plant and Cell Physiology. 35. 519-522 (1994)

[文献書誌] DEMURA,T.: "Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development" Plant Cell. 6. 967-981 (1994)

[文献書誌] ITO,M.: "Meristem-specific gene expression directed by the promotor of the S-phase-specific gene,cyco7" Plant Molecular Biology. 24. 863-878 (1994)

[文献書誌] HAYASHI,T.: "Endo-1,4-β-glucanase in the cell wall of stems of auxintreated pea seedlings" Plant and Cell Physiology. 35. 419-424 (1994)

[文献書誌] BABA,K.: "Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems" Plant and Cell Physiology. 35. 439-444 (1994)

[文献書誌] HAYASHI,T.: "Macromolecular complexes of xyloglucan and cellulose obtained by annealing" Plant and Cell Physiology. 35. 219-223 (1994)

[文献書誌] OHSUMI,C.: "Carbohydrate analysis of an interspecific hybrid between onion and garlic" Biosci.Biotech.Biochem.58. 959-960 (1994)

[文献書誌] KATO,Y.: "Involvement of phenolic esters in cell aggregation of suspension-cultured rice cells" Plant Physiology. 104. 147-152 (1994)

[文献書誌] HOSON,T.: "Concanavalin A inhibits auxin-inducod elongation and hreakdown of(1→3),(1→4)-β-D-glucan in segments of rice coleoptiles" Plant and Cell Physiology. (in press).

[文献書誌] KOBAYASHI,H.: "Involvement of calmodulin and calmodulin-binding proteins in the differentiation of tracheary elements in Zinnia cells" Planta. 194. 388-394 (1994)

[文献書誌] KAWAHARA,R.: "Analysis by in situ hybridization of the expression of elongation factor 1a in the carrot cells during somatic embryogenesis" J.Plant Research. 107. 361-364 (1994)

[文献書誌] FUKUDA,H.: "Mechanisms of the proliferation and differentiation of plant cell culture systems" Intern.J.Developmental Biology. 38. 287-299 (1994)

[文献書誌] SATO,Y.: "Separation and charactorization of the isozymes of wall-bound peroxidase from cultured Zinnia cells during tracheary element differentiation" Planta. (in press).

[文献書誌] KAWAHARA,R.: "Isolation and characterization of homeobox-containing genes of carrot" Plant Molecular Biology. (in press).

[文献書誌] JOY,R.W.IV: "Cloning and characterization of polyphenol oxidase cDNA of Phytolacca americana" Plant Physiology. (in press).

1994 年度実績報告書

柴岡弘郎大阪大学, 理学部, 教授 (60087054)