1993 年度実績報告書

新人為起源有害物質の継世代的人体影響

研究課題

研究課題/領域番号	05278114
研究機関	大阪大学
研究代表者	野村大成大阪大学, 医学部, 教授 (90089871)
研究分担者	藤井儔子帝京大学, 医学部, 教授 (70075224) 木南凌新潟大学, 医学部, 教授 (40133615) 井上雅雄金沢医科大学, 総合医学研究所, 講師 (60064565) 上口勇次郎旭川医科大学, 医学部, 教授 (60091568) 石井裕大阪大学, 医学部, 助教授 (20028509)
キーワード	新人為起源有害物質 / 継世代的影響 / 精子・卵子障害 / 染色体異常 / 発生異常 / 分子遺伝学的異常 / 機能的障害 / 子孫への伝達
研究概要	人類は、科学の進歩とともに、膨大な数の化学物質を地球上に造り出したことにより、人類の歴史上、これまで出くわしたことのない物質に曝露されることになった。本研究は、地球レベルでの情報収集と、最新の科学技術を用いて新人為起源有害物質の継世代的影響を総合的に研究することにより、人類の未来をうかがう研究成果を蓄積し、継世代人体障害の防護の資料とするのを目的とする。本年度は、各分担者が、Dioxin専用の飼育装置の設置、検出方法の決定、いくつかの新人為起源有害物質での試行を行い、以下に示した成果を得た。 1.ヒト精子授精能障害の検出(石井): 合成洗剤は0.02%以上で授精能を100%阻害、ダイオキシンは1μg/mlで30%阻害。 2.アルキル化剤(MMS,TEM)によるヒト精子染色体異常の検出(上口): 切断型異常は直線的、交換型異常は二次曲線的に増加。 3.ダイオキシンのマウス精子、卵子への蓄積とDNA損傷の検出(井上): ^3H-TCDDは少量ながら精巣から検出、電顕的にセルトリ細胞の小胞体の退化が認められたが不定期DNA合成は微少。 4.ミニサテライトを用いた継世代的DNA変異の検出(木南): Pc-1プローブを用い、サザン法によるDNA変異検出法を確立。 5.除草剤による生化学的機能障害の発現(藤井): 除草剤のうちグルホシネートはラットに対し、強い海馬刺激と大脳辺緑系刺激および内分泌系障害を誘発した。 6.雄マウスへの作用によるF_1の致死と奇形(野村): エチルニトロソウレアを父マウスに投与すると、F_1に流産、奇形、仮死が有意に増加。 7.ヒトF_1疫学調査(野村): ベトナムでの父親ダイオキシン被曝による子供の奇形発生についての情報収集を行った。

研究成果

(20件)

すべてその他

すべて文献書誌 (20件)

[文献書誌] Nomura,T.: "Leukaemia in children whose parents have been exposed to radiation." Br.Med.J.306. 1412 (1993)
[文献書誌] Inohara,H.: "Reduction of leaky lymphocyte clones producing immunoglobulins and thymic lymphocytic leukemia by selective inbreeding of scid(severe combined immunodeficiency)mice." Acta Otolaryngol(Stockh). 501. 107-110 (1993)
[文献書誌] Ohsugi,T.: "Isolation of acinetobacter calcoaceticus from the gastrointestinal tracts of SCID mice." Laboratory Animals. 27. 226-228 (1993)
[文献書誌] Tanaka,T.: "The scid mutation in mice couses defects in the repair system for both double-strand DNA breaks and DNA-cross links." Mutation Research. 288. 277-280 (1993)
[文献書誌] Takatera,H.: "Differential expression of a human sperm-specific isozyme in seminoma cells transplanted into scid-nu^<s+r> mice." Jpn.J.Cancer Res.84. 215-218 (1993)
[文献書誌] Sasai,H.: "Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours:detection of transforming genes in X-ray-related mouse tumours." Br.J.Cancer. 67. 262-267 (1993)
[文献書誌] Kamiguchi,Y.: "Chromosomally abnormal gametes as a cause of developmental and congenital anomalies in humans." Cong.Anom.(印刷中). (1993)
[文献書誌] Inazu,N.: "Effects of age and calcium ion on testis crabonyl reductase in rats." Japan J.Pharmacol.63. 65-71 (1993)
[文献書誌] Suzuki,H.: "Compresatory changes in silver-stainability of nucleolar organizer regions in mice." Jpn.J.Genet.67. 217-232 (1993)
[文献書誌] Honama,M.: "A new VNTR-type RFLP probe(ChdTC-15)on chromosome 12(D12S65)." Human Mol.Genet.1. 555 (1993)
[文献書誌] Hayashi,T.: "A variant family of mouse minor satellite located on the centromeric region of chromosome 2." Genomics. 17. 490-492 (1993)
[文献書誌] Suzuki,S.: "Two mouse hyprevariable minisatellites:chromosomal location and simultaneous mutation." J.Biochem.114. 292-296 (1993)
[文献書誌] Enomoto,T.: "Alterations of the Rb gene and its association with ki-ras activation and p53 inactivation in endometrial adenocarcinoma." Molecular Carcinogenesis. 8. 132-137 (1993)
[文献書誌] Enomoto,T.: "Alterations of the p53 tumor suppressor gene and its association with activation of the c-k-ras-2 protooncogene in premalignant and malignant lesions of the human uterine endometrium" Cancer Research. 53. 1883-1888 (1993)
[文献書誌] Gotoh,H.: "Growth inhibition of transplanted murine breast cancer by nicotinamide in C3H/HeJ mice." Cancer Research,Therapy and Control. 3. 121-126 (1993)
[文献書誌] Todo,T.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced(6-4)phtoproducts." Nature. 361. 371-374 (1993)
[文献書誌] Sasai,H.: "Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours:detection of transforming genes in x-ray-related mouse tumours." Br.J.Cancer. 67. 262-267 (1993)
[文献書誌] Kamiguchi,Y.: "Chromosome analysis of unfertilized human oocytes prepared by a gradual fixation-air drying method." Human Genetics. 90. 533-541 (1993)
[文献書誌] Nomura,T.: "Low Dose Radiation and Living State" Narosa Publ., 17 (1993)
[文献書誌] Mikamo,K.: "Chromosomal Aberrations:Origin and Significance" Springer Verlag(印刷中), (1993)

1993 年度 実績報告書

新人為起源有害物質の継世代的人体影響

研究代表者

野村 大成 大阪大学, 医学部, 教授 (90089871)

研究成果

[文献書誌] Nomura,T.: "Leukaemia in children whose parents have been exposed to radiation." Br.Med.J.306. 1412 (1993)

[文献書誌] Inohara,H.: "Reduction of leaky lymphocyte clones producing immunoglobulins and thymic lymphocytic leukemia by selective inbreeding of scid(severe combined immunodeficiency)mice." Acta Otolaryngol(Stockh). 501. 107-110 (1993)

[文献書誌] Ohsugi,T.: "Isolation of acinetobacter calcoaceticus from the gastrointestinal tracts of SCID mice." Laboratory Animals. 27. 226-228 (1993)

[文献書誌] Tanaka,T.: "The scid mutation in mice couses defects in the repair system for both double-strand DNA breaks and DNA-cross links." Mutation Research. 288. 277-280 (1993)

[文献書誌] Takatera,H.: "Differential expression of a human sperm-specific isozyme in seminoma cells transplanted into scid-nu^<s+r> mice." Jpn.J.Cancer Res.84. 215-218 (1993)

[文献書誌] Sasai,H.: "Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours:detection of transforming genes in X-ray-related mouse tumours." Br.J.Cancer. 67. 262-267 (1993)

[文献書誌] Kamiguchi,Y.: "Chromosomally abnormal gametes as a cause of developmental and congenital anomalies in humans." Cong.Anom.(印刷中). (1993)

[文献書誌] Inazu,N.: "Effects of age and calcium ion on testis crabonyl reductase in rats." Japan J.Pharmacol.63. 65-71 (1993)

[文献書誌] Suzuki,H.: "Compresatory changes in silver-stainability of nucleolar organizer regions in mice." Jpn.J.Genet.67. 217-232 (1993)

[文献書誌] Honama,M.: "A new VNTR-type RFLP probe(ChdTC-15)on chromosome 12(D12S65)." Human Mol.Genet.1. 555 (1993)

[文献書誌] Hayashi,T.: "A variant family of mouse minor satellite located on the centromeric region of chromosome 2." Genomics. 17. 490-492 (1993)

[文献書誌] Suzuki,S.: "Two mouse hyprevariable minisatellites:chromosomal location and simultaneous mutation." J.Biochem.114. 292-296 (1993)

[文献書誌] Enomoto,T.: "Alterations of the Rb gene and its association with ki-ras activation and p53 inactivation in endometrial adenocarcinoma." Molecular Carcinogenesis. 8. 132-137 (1993)

[文献書誌] Enomoto,T.: "Alterations of the p53 tumor suppressor gene and its association with activation of the c-k-ras-2 protooncogene in premalignant and malignant lesions of the human uterine endometrium" Cancer Research. 53. 1883-1888 (1993)

[文献書誌] Gotoh,H.: "Growth inhibition of transplanted murine breast cancer by nicotinamide in C3H/HeJ mice." Cancer Research,Therapy and Control. 3. 121-126 (1993)

[文献書誌] Todo,T.: "A new photoreactivating enzyme that specifically repairs ultraviolet light-induced(6-4)phtoproducts." Nature. 361. 371-374 (1993)

[文献書誌] Sasai,H.: "Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours:detection of transforming genes in x-ray-related mouse tumours." Br.J.Cancer. 67. 262-267 (1993)

[文献書誌] Kamiguchi,Y.: "Chromosome analysis of unfertilized human oocytes prepared by a gradual fixation-air drying method." Human Genetics. 90. 533-541 (1993)

[文献書誌] Nomura,T.: "Low Dose Radiation and Living State" Narosa Publ., 17 (1993)

[文献書誌] Mikamo,K.: "Chromosomal Aberrations:Origin and Significance" Springer Verlag(印刷中), (1993)

1993 年度実績報告書

野村大成大阪大学, 医学部, 教授 (90089871)