1993 年度実績報告書

粘菌細胞の分化と形態形成の制御機構

研究課題

研究課題/領域番号	05304057
研究機関	岡崎国立共同研究機構
研究代表者	竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)
研究分担者	岡本浩二京都大学, 理学部, 助教授 (10029944) 田仲可昌筑波大学, 生物科学系, 教授 (80091908) 柳澤嘉一郎筑波大学, 生物科学系, 教授 (60015899) 前田靖男東北大学, 理学部, 教授 (50025417) 落合廣北海道大学, 理学部, 教授 (10002122)
キーワード	細胞分化 / 形態形成 / 細胞接着 / 遺伝子発現 / 細胞周期 / 細胞性粘菌
研究概要	細胞性粘菌の発生系を対象として以下の研究を行った。形態形成の制御機構細胞接着に関わる分子gp64とCsAの糖脂質および糖鎖の構造を明らかにし、細胞接着における役割を論じた(落合・吉田元信)。ブラストシジンS耐性株を用いる挿入突然変異体の分離法を確立し(田仲)、それを用いて有性生殖における細胞融合能の低い形質転換体を得た(柳澤)。細胞膜-細胞骨格標品を用いて、細胞運動関連蛋白質の動的構築変化を調べ、細胞内カルシュウムの関与を明らかにした(裕村恵彦)。cAMP刺激に対して細胞内カルシュウムが増加すること、予定柄細胞が予定胞子細胞よりも大きな反応を示すことを明らかにした(竹内)。細胞内cGMP量が細胞の高浸浸透処理によって増加することを見出した(小山正和)。細胞分化の制御機構飢餓処理時点での細胞の細胞周期上の位置が特異点(増殖・分化の分岐点)の直前にある細胞は集合中心として機能したのちに予定胞子細胞に分化し、直後の細胞は遅れて集合し、予定柄細胞に分化することを明らかにした(前田)。細胞の集合時に細胞密度に依存して発現する新たな遺伝子(dutA)を見出した。このRNAは翻訳されないが、広く他の生物にも存在する新しい種類のものである(岡本)。低細胞密度の細胞に分化を誘導する因子を培養上清中に見出し、それが70-80kDの蛋白質であることを示した。この因子は分化過程で細胞分裂をも誘導する(大畠明子)。予定胞子細胞に特異的に発現する遺伝子Dp87は集合前期の細胞にその位置と無関係に発現し、発現した細胞は移動体の予定胞子細胞域に選別される(田坂昌生)。

研究成果

(22件)

すべてその他

すべて文献書誌 (22件)

[文献書誌] Ozaki T.: "Developmental regulation of transcription of a novel prespore‐specific gene(Dp87)in Dictyostelium discoideum" Development. 117. 1299-1308 (1993)
[文献書誌] Morio T.: "Cooperation of positively and negatively acting promoter elements determines prespore‐specific transcription of Dp87 gene in Dictyosetlium" Mechanisms of Development. in press. (1994)
[文献書誌] Nakao H.: "Dictyostelium prespore‐specific gene(Dp87)encodes a sorus matrix protein" Journal of Cell science. in press. (1994)
[文献書誌] Saran S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transfomed with jelly‐fish apoaequorin cDNA" FEBS Letters. 337. 43-47 (1994)
[文献書誌] Traynor D.: "A berrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. in press. (1994)
[文献書誌] Saito T.: "A purification method and N‐glycosylation sites of a 36‐cysteine‐containing,putative cell/cell adhesion glycoprotein gp64 of the cellular slime mold,Polysphondylium pallidum" Eur.J.Biochem. 211. 147-155 (1993)
[文献書誌] Morita M.: "DNA packaging ATPase of bacteriophage T3" Virology. 193. 748-752 (1993)
[文献書誌] Morita M.: "Analysis of functional domains of the packaging proteins of bacteriophage T3 by site‐directed mutagenesis" J.Mol.Biol.in press. (1994)
[文献書誌] Morita S.: "A cDNA encoding a rice catalase isozyme" Plant Physiology. in press. (1994)
[文献書誌] Agarwal A.: "Analysis of a novel cyclic AMP inducible prespore gene in Dictyostelium discoideum:Evidence for different patterns of cAMP regulation" Differentiation. in press. (1994)
[文献書誌] Kubohara Y.: "Analysis of the maturation process of prestalk cells in Dictyostelium discoideum" Exp.Cell Res.207. 107-114 (1993)
[文献書誌] Yamada Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Developmental Biology. 161. 296-301 (1994)
[文献書誌] Yoshida H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.Acids Res.22. 41-46 (1994)
[文献書誌] Yoshida M.: "Carbohydrate structures of the cell adhesion molecule, contact site A,from Dictyostelium discoideum" FEBS Letters. 318. 305-309 (1993)
[文献書誌] Yumura S.: "A mechanism for the intracellular localization of myosin II filaments in the Dictyostelium amoeba" Journal of Cell Science. 105. 233-242 (1993)
[文献書誌] Yumura S.: "Reorganization of actin and myosin II in Dictyostelium amoeba during stimulation by cAMP" Cell Struct.Funct.in press. (1994)
[文献書誌] Saito T.: "Evidence for a glycolipid anchor of gp64,a putative cell‐cell adhesion protein of Polysphondylium pallidum" Eur.J.Biochem.218. 623-628 (1993)
[文献書誌] Maeda Y.: "Pattern formation in a cell‐cycle dependent manner during the development of Dictyostelium discoideum" Develop.Growth & Differ.35. 609-616 (1993)
[文献書誌] Fang H.: "Molecular cloning and chacterization of two genes encoding gp138,a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum" Developmental Biology. 156. 201-208 (1993)
[文献書誌] Fang H.: "Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum" Journal of Cell Science. 106. 785-788 (1993)
[文献書誌] Kubohara Y.: "Differanisole A,an inducer of the differentiation of friend leukemic cells,induces stalk cell differentiation in Dictyostelium discoideum" FEBS Letters. 322. 73-75 (1993)
[文献書誌] Kubohara Y.: "DIF‐resistant expression of a prespore‐specific gene in Dictyostelium in vitro development" Exp.Cell Res.204. 382-384 (1993)

1993 年度 実績報告書

粘菌細胞の分化と形態形成の制御機構

研究代表者

竹内 郁夫 岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)

研究成果

[文献書誌] Ozaki T.: "Developmental regulation of transcription of a novel prespore‐specific gene(Dp87)in Dictyostelium discoideum" Development. 117. 1299-1308 (1993)

[文献書誌] Morio T.: "Cooperation of positively and negatively acting promoter elements determines prespore‐specific transcription of Dp87 gene in Dictyosetlium" Mechanisms of Development. in press. (1994)

[文献書誌] Nakao H.: "Dictyostelium prespore‐specific gene(Dp87)encodes a sorus matrix protein" Journal of Cell science. in press. (1994)

[文献書誌] Saran S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transfomed with jelly‐fish apoaequorin cDNA" FEBS Letters. 337. 43-47 (1994)

[文献書誌] Traynor D.: "A berrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. in press. (1994)

[文献書誌] Saito T.: "A purification method and N‐glycosylation sites of a 36‐cysteine‐containing,putative cell/cell adhesion glycoprotein gp64 of the cellular slime mold,Polysphondylium pallidum" Eur.J.Biochem. 211. 147-155 (1993)

[文献書誌] Morita M.: "DNA packaging ATPase of bacteriophage T3" Virology. 193. 748-752 (1993)

[文献書誌] Morita M.: "Analysis of functional domains of the packaging proteins of bacteriophage T3 by site‐directed mutagenesis" J.Mol.Biol.in press. (1994)

[文献書誌] Morita S.: "A cDNA encoding a rice catalase isozyme" Plant Physiology. in press. (1994)

[文献書誌] Agarwal A.: "Analysis of a novel cyclic AMP inducible prespore gene in Dictyostelium discoideum:Evidence for different patterns of cAMP regulation" Differentiation. in press. (1994)

[文献書誌] Kubohara Y.: "Analysis of the maturation process of prestalk cells in Dictyostelium discoideum" Exp.Cell Res.207. 107-114 (1993)

[文献書誌] Yamada Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Developmental Biology. 161. 296-301 (1994)

[文献書誌] Yoshida H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.Acids Res.22. 41-46 (1994)

[文献書誌] Yoshida M.: "Carbohydrate structures of the cell adhesion molecule, contact site A,from Dictyostelium discoideum" FEBS Letters. 318. 305-309 (1993)

[文献書誌] Yumura S.: "A mechanism for the intracellular localization of myosin II filaments in the Dictyostelium amoeba" Journal of Cell Science. 105. 233-242 (1993)

[文献書誌] Yumura S.: "Reorganization of actin and myosin II in Dictyostelium amoeba during stimulation by cAMP" Cell Struct.Funct.in press. (1994)

[文献書誌] Saito T.: "Evidence for a glycolipid anchor of gp64,a putative cell‐cell adhesion protein of Polysphondylium pallidum" Eur.J.Biochem.218. 623-628 (1993)

[文献書誌] Maeda Y.: "Pattern formation in a cell‐cycle dependent manner during the development of Dictyostelium discoideum" Develop.Growth & Differ.35. 609-616 (1993)

[文献書誌] Fang H.: "Molecular cloning and chacterization of two genes encoding gp138,a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum" Developmental Biology. 156. 201-208 (1993)

[文献書誌] Fang H.: "Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum" Journal of Cell Science. 106. 785-788 (1993)

[文献書誌] Kubohara Y.: "Differanisole A,an inducer of the differentiation of friend leukemic cells,induces stalk cell differentiation in Dictyostelium discoideum" FEBS Letters. 322. 73-75 (1993)

[文献書誌] Kubohara Y.: "DIF‐resistant expression of a prespore‐specific gene in Dictyostelium in vitro development" Exp.Cell Res.204. 382-384 (1993)

1993 年度実績報告書

竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)